ID   W22040; SV 1; linear; mRNA; EST; HUM; 614 BP.
XX
AC   W22040;
XX
DT   09-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   60E3 Human retina cDNA Tsp509I-cleaved sublibrary Homo sapiens cDNA not
DE   directional, mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-614
RA   Macke J., Smallwood P., Nathans J.;
RT   "Adult Human Retina cDNA";
RL   Unpublished.
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DR   MD5; 1207a89c5656b433f1530312a18a468e.
DR   UNILIB; 542; 432.
XX
CC   On Apr 14, 1993 this sequence version replaced gi:785648.
CC   Contact: Dr. Jeremy Nathans
CC   Dr. Jeremy Nathans, Dept. of Molecular Biology and Genetics
CC   Johns Hopkins School of Medicine
CC   725 North Wolfe Street, Baltimore, MD 21205
CC   Tel: 410 955 4678
CC   Fax: 410 614 0827
CC   Email: jeremy_nathans@qmail.bs.jhu.edu
CC   Clones from this library are NOT available.
CC   PCR PRimers
CC   FORWARD: CTTTTGAGCAAGTTCAGCCTGGTTAAGT
CC   BACKWARD: GAGGTGGCTTATGAGTATTTCTTCCAGGGTAA
CC   Seq primer: GGGTAAAAAGCAAAAGAATT.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..614
FT                   /organism="Homo sapiens"
FT                   /lab_host="E. coli strain K802"
FT                   /mol_type="mRNA"
FT                   /sex="mixed (males and females)"
FT                   /dev_stage="adult"
FT                   /clone_lib="Human retina cDNA Tsp509I-cleaved sublibrary"
FT                   /tissue_type="retina"
FT                   /note="Organ: eye; Vector: lambda gt10; Site_1: EcoRI;
FT                   Site_2: EcoRI; The library used for sequencing was a
FT                   sublibrary derived from a human retina cDNA library.
FT                   Inserts from retina cDNA library DNA were isolated, cleaved
FT                   with Tsp509I, size selected, and cloned into lamda gt10. 
FT                   Individual plaques were arrayed and used as templates for
FT                   PCR amplification and these PCR products were used for
FT                   sequencing."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:542"
XX
SQ   Sequence 614 BP; 125 A; 136 C; 188 G; 148 T; 17 other;
     aagtccaagg tgaattggct ggaaaatgtc cctgaagnct ggatngacac ctgagcannc        60
     ttccnagtcc tgggaaatgt ggctgctcca tatttggaca gagcatggag agccaccact       120
     gtgtcctggg tggaggagaa accgccctgg gcattctgct gcttcgtgat ccacttcacg       180
     atgttggttg cagaggtcag gtcctccgag gttggggctg gctgggccgt gagataagcg       240
     aggagcacat aggatgtcat ctccacctca gcagagggag cctggggttc gtaaaaatgc       300
     cccactgggg ccttgggttt cttgagggcg ctcccatggn aaagagttgt ctttcttcac       360
     agcttcctca ttaagtgact tgagtacttc cttcctcttg tcctggttac ctgccagggc       420
     aaaaagcata ggccagcang tgctttgggt ttattcatng gttgccatgg tccccttctt       480
     ggtgttgtct tccccagggc ttgattccca ggnaaaaaca ggggaattgg ggacaacagg       540
     ggtgantgga ttgtganang gatcntccag aagggngatg ggggattttg ggggggaggg       600
     gcaaacactn cnnc                                                         614
//