ID   W19003; SV 1; linear; mRNA; EST; MUS; 269 BP.
XX
AC   W19003;
XX
DT   05-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 3)
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DE   mc11e02.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:348218 5'
DE   similar to gb:U01063 Mus musculus pLK serine/threonine kinase mRNA,
DE   complete (MOUSE);, mRNA sequence.
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KW   EST.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-269
RA   Marra M., Hillier L., Allen M., Bowles M., Dietrich N., Dubuque T.,
RA   Geisel S., Kucaba T., Lacy M., Le M., Martin J., Morris M.,
RA   Schellenberg K., Steptoe M., Tan F., Underwood K., Moore B., Theising B.,
RA   Wylie T., Lennon G., Soares B., Wilson R., Waterston R.;
RT   "The WashU-HHMI Mouse EST Project";
RL   Unpublished.
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DR   MD5; be31347f23cbcd0a81487b21b9ea78c6.
DR   UNILIB; 637; 431.
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CC   On Nov 29, 1993 this sequence version replaced gi:430008.
CC   Contact: Marra M/Mouse EST Project
CC   WashU-HHMI Mouse EST Project
CC   Washington University School of MedicineP
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: mouseest@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   MGI:220018
CC   Seq primer: mob.REGA+ET
CC   High quality sequence stop: 241.
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FH   Key             Location/Qualifiers
FH
FT   source          1..269
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /dev_stage="19.5 dpc total fetus"
FT                   /clone_lib="Soares mouse p3NMF19.5"
FT                   /clone="IMAGE:348218"
FT                   /note="Vector: pT7T3D (Pharmacia) with a modified
FT                   polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
FT                   was primed with a Not I - oligo(dT) primer [5'
FT                   TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). Library went through one round of
FT                   normalization to a Cot = 5. Library constructed by Bento
FT                   Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr.
FT                   Minoru Ko (Wayne State University)."
FT                   /db_xref="taxon:10090"
FT                   /db_xref="UNILIB:637"
XX
SQ   Sequence 269 BP; 60 A; 71 C; 85 G; 53 T; 0 other;
     ctcaataaag gtgtggagaa ccccctgcct gaccgtcccc gggagaaaga ggaaccggtg        60
     gtccgggaga caaatgaggc cattgagtgc caccttagtg acttgctaca gcagctgacc       120
     agtgtcaacg cctccaagcc ctcggagcgc gggctggtgc ggcaagagga ggctgaggat       180
     cctgcctgca tccccatctt ctgggtcagc aagtgggtgg actattcgga caagtatggc       240
     cttgggtatc agctgtgtta caacagtgt                                         269
//