ID   W18632; SV 1; linear; mRNA; EST; MUS; 184 BP.
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AC   W18632;
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DT   05-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 3)
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DE   mb78e01.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:335544 5',
DE   mRNA sequence.
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KW   EST.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-184
RA   Marra M., Hillier L., Allen M., Bowles M., Dietrich N., Dubuque T.,
RA   Geisel S., Kucaba T., Lacy M., Le M., Martin J., Morris M.,
RA   Schellenberg K., Steptoe M., Tan F., Underwood K., Moore B., Theising B.,
RA   Wylie T., Lennon G., Soares B., Wilson R., Waterston R.;
RT   "The WashU-HHMI Mouse EST Project";
RL   Unpublished.
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DR   MD5; c29d6d7122c220b3885fc125cbbcf2df.
DR   UNILIB; 637; 431.
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CC   On May 9, 1995 this sequence version replaced gi:802618.
CC   Contact: Marra M/Mouse EST Project
CC   WashU-HHMI Mouse EST Project
CC   Washington University School of MedicineP
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: mouseest@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   MGI:216944
CC   Seq primer: mob.REGA+ET
CC   High quality sequence stop: 173.
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FH   Key             Location/Qualifiers
FH
FT   source          1..184
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /dev_stage="19.5 dpc total fetus"
FT                   /clone_lib="Soares mouse p3NMF19.5"
FT                   /clone="IMAGE:335544"
FT                   /note="Vector: pT7T3D (Pharmacia) with a modified
FT                   polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
FT                   was primed with a Not I - oligo(dT) primer [5'
FT                   TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). Library went through one round of
FT                   normalization to a Cot = 5. Library constructed by Bento
FT                   Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr.
FT                   Minoru Ko (Wayne State University)."
FT                   /db_xref="taxon:10090"
FT                   /db_xref="UNILIB:637"
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SQ   Sequence 184 BP; 56 A; 33 C; 39 G; 56 T; 0 other;
     caataaaaat gtacaggtgg gacagtgttt tagtgacagg cttcattttc tttaattgaa        60
     ctgttactca ttgtgttaga agtaactgta cacacataaa atgggcatcc aggaggtata       120
     gatagcagca gtcctttata gaatggtctg tacatcagga agtaatacgc atccccctgc       180
     cttt                                                                    184
//