ID   W18361; SV 1; linear; mRNA; EST; MUS; 332 BP.
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AC   W18361;
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DT   04-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 3)
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DE   mb69h12.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:334727 5',
DE   mRNA sequence.
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KW   EST.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-332
RA   Marra M., Hillier L., Allen M., Bowles M., Dietrich N., Dubuque T.,
RA   Geisel S., Kucaba T., Lacy M., Le M., Martin J., Morris M.,
RA   Schellenberg K., Steptoe M., Tan F., Underwood K., Moore B., Theising B.,
RA   Wylie T., Lennon G., Soares B., Wilson R., Waterston R.;
RT   "The WashU-HHMI Mouse EST Project";
RL   Unpublished.
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DR   MD5; 07659a6cec26f084affdeb800b6a8421.
DR   UNILIB; 637; 431.
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CC   On May 8, 1995 this sequence version replaced gi:801504.
CC   Contact: Marra M/Mouse EST Project
CC   WashU-HHMI Mouse EST Project
CC   Washington University School of MedicineP
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: mouseest@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   MGI:216127
CC   Seq primer: ETPrimer
CC   High quality sequence stop: 309.
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FH   Key             Location/Qualifiers
FH
FT   source          1..332
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /dev_stage="19.5 dpc total fetus"
FT                   /clone_lib="Soares mouse p3NMF19.5"
FT                   /clone="IMAGE:334727"
FT                   /note="Vector: pT7T3D (Pharmacia) with a modified
FT                   polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
FT                   was primed with a Not I - oligo(dT) primer [5'
FT                   TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). Library went through one round of
FT                   normalization to a Cot = 5. Library constructed by Bento
FT                   Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr.
FT                   Minoru Ko (Wayne State University)."
FT                   /db_xref="taxon:10090"
FT                   /db_xref="UNILIB:637"
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SQ   Sequence 332 BP; 85 A; 87 C; 79 G; 81 T; 0 other;
     ggttaactca ggttttgaaa acactcccac accatgtggg ctggggagaa gacattttac        60
     ctcctccctc cagcaccgct caaaagttgg acatttctca caaaagccat gttatatctc       120
     aaaacaacta gagtgcgtag tagggcccgt gactagcagc tccttcctgc gctggttaaa       180
     ttagaacagc tgaatgctaa gttattggca ctttacacgt tactgtcacc atgtgagagc       240
     accggagtcg cggctcctag gccagcagtg ggaggtcaga ggccttcgag cctcgttctt       300
     tatcacagct gtgagtgcaa gtcactagca ac                                     332
//