ID   W17191; SV 1; linear; mRNA; EST; HUM; 480 BP.
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AC   W17191;
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DT   04-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   zb18e11.r1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone IMAGE:302444
DE   5' similar to gb:D28480 MCM3 HOMOLOG (HUMAN);, mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-480
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
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DR   MD5; 518293830e78ec4a88481886b5b5349a.
DR   UNILIB; 121; 415.
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CC   On Apr 18, 1995 this sequence version replaced gi:775506.
CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Seq primer: ETPrimer
CC   High quality sequence stop: 386.
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FH   Key             Location/Qualifiers
FH
FT   source          1..480
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /dev_stage="19 weeks"
FT                   /clone_lib="Soares_fetal_lung_NbHL19W"
FT                   /clone="IMAGE:302444"
FT                   /note="Organ: lung; Vector: pT7T3D (Pharmacia) with a
FT                   modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
FT                   strand cDNA was primed with a Not I - oligo(dT) primer
FT                   [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). Library went through one round of
FT                   normalization to a Cot = 5. Library constructed by Bento
FT                   Soares and M.Fatima Bonaldo. This library was constructed
FT                   from the same fetus as the fetal heart library, Soares
FT                   fetal heart NbHH19W."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:121"
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SQ   Sequence 480 BP; 122 A; 132 C; 120 G; 102 T; 4 other;
     tgacaatcta caaacacttt tattagcaaa aggagtaagt gcagcatggg agaaagaggg        60
     gctcctcctt tcccctcaaa ggcatcactg ccccttccca aggggcaggc cagcagggaa       120
     caagaggacc ccagggttgc aagcaggctg gaatcagaca aaagtgatcc gtgtccggga       180
     agcattgacc tgccagacat tgagctcctc atattcatcc agagccgcct ggaactgggc       240
     gggtgtgaag ccacgagata cacagcgctg ctctgcctca gagaaccgga ntcttcggcc       300
     ccctgagacc agttcacgga cggtggcaaa tatcacatct gctggtctct gagtcctagc       360
     tgtctgcccc ttgtctccta gaagagagtc ctttgacatt ccattagcct gatggttcat       420
     tccatttttn ctcaccacat caccatttca gacgtgcaga nagtggaaag gcncagatag       480
//