ID   W14482; SV 1; linear; mRNA; EST; MUS; 280 BP.
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AC   W14482;
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DT   30-APR-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 3)
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DE   mb30c03.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:330916 5'
DE   similar to gb:S90469 NADPH-CYTOCHROME P450 REDUCTASE (HUMAN); gb:D17571
DE   Mouse mRNA for NADPH-cytochrome P450 oxidoreductase, complete (MOUSE);,
DE   mRNA sequence.
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KW   EST.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-280
RA   Marra M., Hillier L., Allen M., Bowles M., Dietrich N., Dubuque T.,
RA   Geisel S., Kucaba T., Lacy M., Le M., Martin J., Morris M.,
RA   Schellenberg K., Steptoe M., Tan F., Underwood K., Moore B., Theising B.,
RA   Wylie T., Lennon G., Soares B., Wilson R., Waterston R.;
RT   "The WashU-HHMI Mouse EST Project";
RL   Unpublished.
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DR   MD5; 5e67f3739a96f62a72d2a157f37c0880.
DR   UNILIB; 637; 431.
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CC   On Sep 12, 1996 this sequence version replaced gi:1288657.
CC   Contact: Marra M/Mouse EST Project
CC   WashU-HHMI Mouse EST Project
CC   Washington University School of MedicineP
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: mouseest@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   MGI:212316
CC   Trace considered overall poor quality
CC   Seq primer: -28M13 rev2 from Amersham
CC   High quality sequence stop: 1.
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FH   Key             Location/Qualifiers
FH
FT   source          1..280
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /dev_stage="19.5 dpc total fetus"
FT                   /clone_lib="Soares mouse p3NMF19.5"
FT                   /clone="IMAGE:330916"
FT                   /note="Vector: pT7T3D (Pharmacia) with a modified
FT                   polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
FT                   was primed with a Not I - oligo(dT) primer [5'
FT                   TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). Library went through one round of
FT                   normalization to a Cot = 5. Library constructed by Bento
FT                   Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr.
FT                   Minoru Ko (Wayne State University)."
FT                   /db_xref="taxon:10090"
FT                   /db_xref="UNILIB:637"
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SQ   Sequence 280 BP; 65 A; 95 C; 67 G; 53 T; 0 other;
     acacccaggc tgtggactat gttaagaagc tcatgaccaa gggccgctac tcgctggatg        60
     tatggagcta agaactgccg ccccccaccc ctcgctccct gaaatcacgt ccttaacttc       120
     cttccgacga cctccaactc tggcgtaacc tgccctgcct ggacacaggg atgcccaggg       180
     actgactcct ggcctgagtg atgccctcct aggcgcctta agcacaagca tggtcacata       240
     tgtaacaagg caggactagc ctcaacccag ggcacatggc                             280
//