ID U32369; SV 1; circular; genomic DNA; STD; PRO; 4630 BP. XX AC U32369; XX DT 04-JAN-1996 (Rel. 46, Created) DT 10-APR-2020 (Rel. 144, Last updated, Version 6) XX DE Bacillus cereus plasmid pBC16, complete sequence. XX KW cmp; copT; mob; mobilization protein; oriL; oriU; replication protein; KW repU; tet; tetracycline resistance. XX OS Bacillus cereus OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OG Plasmid pBC16 XX RN [1] RP 1-4630 RA Tang M.R., Wilson C.R.; RT "The Nucleotide Sequence of Plasmid pBC16 and its Relationship to Plasmid RT pUB110"; RL Unpublished. XX RN [2] RP 1-4630 RA Wilson C.R.; RT ; RL Submitted (26-JUL-1995) to the INSDC. RL C. Ron Wilson, COGNIS, Inc., Bioproducts, 2331 Circadian Way, Santa Rosa, RL CA 95407-5415, USA XX DR MD5; e8d2ed80ba9107a665b7ca34c778d926. DR BioSample; SAMN14228825. DR EuropePMC; PMC92446; 11097892. XX FH Key Location/Qualifiers FH FT source 1..4630 FT /organism="Bacillus cereus" FT /plasmid="pBC16" FT /lab_host="Bacillus subtilis" FT /mol_type="genomic DNA" FT /db_xref="taxon:1396" FT CDS complement(join(4442..4630,1..1137)) FT /codon_start=1 FT /transl_table=11 FT /product="Mob" FT /note="ORF beta" FT /db_xref="GOA:Q51989" FT /db_xref="InterPro:IPR001668" FT /db_xref="UniProtKB/TrEMBL:Q51989" FT /protein_id="AAA84921.1" FT /translation="MVAGKSENASHLCHLKRSDLHMSYAVCRMQKVKSAGLKGMQFHNQ FT RERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELL FT VTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRD FT GKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEID FT FLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPT FT KNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVER FT FNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVD FT KVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL" FT gene complement(join(4442..4630,1..1074)) FT /gene="mob" FT CDS complement(join(4442..4630,1..1074)) FT /codon_start=1 FT /transl_table=11 FT /gene="mob" FT /product="Mob protein" FT /function="required for plasmid mobilization" FT /note="proposed coding region for Mob, downstream from a FT putative RBS" FT /db_xref="GOA:Q79CL1" FT /db_xref="InterPro:IPR001668" FT /db_xref="UniProtKB/TrEMBL:Q79CL1" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA84922.1" FT /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD FT LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE FT ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF FT PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK FT SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND FT RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK FT ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE FT FELTHNREVKKERSRDQGMSL" FT misc_feature 1..780 FT /standard_name="BA4" FT /note="membrane binding region; BA4 was identified in FT plasmid pUB110 by Tanaka et al. (1983) as exhibiting Type FT II in-vitro binding to membrane preparations" FT regulatory 1040..1549 FT /standard_name="cmp locus" FT /function="proposed to enhance interaction between RepU and FT oriU" FT /note="DNA sequence in pUB110 proposed to enhance FT interaction between RepU protein and oriU" FT /regulatory_class="other" FT rep_origin 1245..1521 FT /standard_name="oriL" FT /note="On plasmid pUB110 the minor origin of replication FT (oriL)" FT misc_feature 1358..1549 FT /standard_name="BA3" FT /note="membrane binding region; BA3 was identified in FT plasmid pUB110 by Tanaka et al. (1983) as exhibiting Type FT II in-vitro binding to membrane preparations" FT gene complement(1638..3026) FT /gene="tet" FT CDS complement(1638..3014) FT /codon_start=1 FT /transl_table=11 FT /gene="tet" FT /product="tetracycline resistance protein" FT /function="provides resistance to tetracycline" FT /db_xref="GOA:Q79CL3" FT /db_xref="InterPro:IPR001411" FT /db_xref="InterPro:IPR011701" FT /db_xref="InterPro:IPR020846" FT /db_xref="InterPro:IPR036259" FT /db_xref="UniProtKB/TrEMBL:Q79CL3" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA84918.1" FT /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN FT KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS FT LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA FT HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS FT ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY FT MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA FT SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT FT GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF FT " FT regulatory complement(3018..3026) FT /gene="tet" FT /note="putative RBS (ggagggtga) positioned just upstream of FT the tet gene" FT /regulatory_class="ribosome_binding_site" FT misc_feature 3210..3525 FT /standard_name="BA2" FT /note="membrane binding region; BA2 was identified in FT plasmid pUB110 by Tanaka et al. (1983) as exhibiting Type FT II in-vitro binding to membrane preparations" FT CDS complement(3214..4218) FT /codon_start=1 FT /transl_table=11 FT /product="RepU" FT /note="ORF alpha" FT /db_xref="GOA:Q51988" FT /db_xref="InterPro:IPR000989" FT /db_xref="UniProtKB/TrEMBL:Q51988" FT /protein_id="AAA84919.1" FT /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY FT FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI FT QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV FT GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN FT VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS FT YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE" FT gene complement(3214..4197) FT /gene="repU" FT CDS complement(3214..4197) FT /codon_start=1 FT /transl_table=11 FT /gene="repU" FT /product="RepU" FT /function="required for plasmid replication" FT /note="evidence indicates that this is the position of the FT RepU protein coding region" FT /db_xref="GOA:Q51987" FT /db_xref="InterPro:IPR000989" FT /db_xref="UniProtKB/TrEMBL:Q51987" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA84920.1" FT /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHIL FT EFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVA FT EVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMRATE FT VTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKVQMIR FT PKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYGGLLKE FT IHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE" FT misc_feature 3565..4115 FT /standard_name="BA1" FT /note="membrane binding region; The BA1 region in plasmid FT pUB110 was identified by Tanaka et al. (1983) as exhibiting FT Type II in-vitro binding to membrane preparations" FT regulatory 4114..4295 FT /standard_name="copT locus" FT /function="determines plasmid incompatibility to other FT plasmids" FT /note="copT in pUB110 is involved in plasmid FT incompatibility. The same sequence is present in plasmid FT pBC16. The copT locus borders the beginning of repU and FT includes the putative stem-loop structure" FT /regulatory_class="other" FT stem_loop 4209..4266 FT /function="involved in control of plasmid replication" FT /note="Putative stem-loop structure present in mRNA which FT may influence synthesis of RepU protein, thereby FT controlling plasmid copy number" FT rep_origin 4370..4396 FT /standard_name="oriU" XX SQ Sequence 4630 BP; 1433 A; 952 C; 632 G; 1613 T; 0 other; gaattcctta gtgctttcat agattaaact cacatcacgc tttaaatcgc ttattttaga 60 ctttaaagac ttgttttctt caagcaactc attataatca tttacatttt cattaaatcg 120 ctctacaaga ccactatatt tttctttaac ttgcccatgt tctttactta attttttata 180 ttctctcgcc atatcagtac tcatgagatt tctaacatgc tgttttaacc tatcgttatc 240 tctcgcagca gtcactaagt ttttataatc acgctccgat ataacaacat ttttggttgg 300 tttcttttct gttttcatta tttcttttcc caaaccaaac atggactttt cacccgttgg 360 cacttcaaca cttttcatgt gtcgtttcgc tggtacttct aaatctgatt taactttatc 420 gctataagca gtccattcat cttttttaac tgctaaattt ttttctagaa aatcaatctc 480 tttttccaaa gtttgttttt taaatttagc tgtctcaata tgtttacggt cagagccacg 540 ttcaccacgc ttcaactcaa aaccctgttt tttcatatgc tcggggaatt tatcttgtag 600 ccataacagt tcttgacgat taaacacatt ttttccttgc agttttccat cacgcatagg 660 cacaacacct aaatgcatgt gaggggtttg ctcatcatta tgaactgttg cataagcaat 720 attttgcttg ccatatcgtt cggaaaataa tttataactt tcctcaaaaa atcgtttttg 780 ttctcctgga tccagttgct caaaaaaatc tcggtcagat gttactagca actcatttac 840 aagaacagca tctttcctcg tttttcttgt acctgttttt tgtgattcaa taatttcttt 900 gacacgttcg ttgtaatcaa tatttttatc atttttcaaa tcataatttt cacgtgttcg 960 ctcatggtca atatcatcat tcgttctact ttttcgctct ctttgattat gaaattgcat 1020 gccttttagt ccagctgatt tcactttttg cattctacaa actgcataac tcatatgtaa 1080 atcgctcctt tttaggtggc acaaatgtga ggcattttcg ctctttccgg caaccacttc 1140 caagtaaagt ataacacact atactttata ttcataaagt gtgtgctctg cgaggctgtc 1200 ggcagtgccg accaaaacca taaaaccttt aagacctttc ttttttttac gagaaaaaag 1260 aaacaaaaaa acctgccctc tgccacctca gcaaaggggg gttttgctct cgtgctcgtt 1320 taaaaatcag caagggacag gtagtatttt ttgagaagat cactcaaaaa atctccacct 1380 ttaaaccctt gccaattttt attttgtccg ttttgtctag cttaccgaaa gccagactca 1440 gcaagaataa aatttttatt gtctttcggt tttctagtgt aacggacaaa accactcaaa 1500 ataaaaaaga tacaagagag gtctctcgta tcttttattc agcaatcgcg ccctttaatg 1560 gaacaaaaag atttgaattc ctgttataaa aaaaggatca attttgaact ctctcccaaa 1620 gttgatccct taacgattta gaaatccctt tgagaatgtt tatatacatt caaggtaacc 1680 agccaactaa tgacaatgat tcctgaaaaa agtaataaca aattactata cagataagtt 1740 gactgatcaa cttccatagg taacaacctt tgatcaagta agggtatgga taataaacca 1800 cctacaattg caatacctgt tccctctgat aaaaagctgg taaagttaag caaactcatt 1860 ccagcaccag cttcctgctg tttcaagcta cttgaaacaa ttgttgatat aactgttttg 1920 gtgaacgaaa gcccacctaa aacaaatacg attataattg tcatgaacca tgatgttgtt 1980 tctaaaagaa aggaagcagt taaaaagcta acagaaagaa atgtaactcc gatgtttaac 2040 acgtataaag gacctcttct atcaacaagt atcccaccaa tgtagccgaa aataatgaca 2100 ctcattgttc cagggaaaat aattacactt ccgatttcgg cagtacttag ctggtgaaca 2160 tctttcatca tataaggaac catagagaca aaccctgcta ctgttccaaa tataattccc 2220 ccacaaagaa ctccaatcat aaaaggtata tttttcccta atccgggatc aacaaaagga 2280 tctgttactt tcctgatatg ttttacaaat atcaggaatg acagcacgct aacgataaga 2340 aaagaaatgc tatatgatgt tgtaaacaac ataaaaaata caatgcctac agacattagt 2400 ataattcctt tgatatcaaa atgacctttt atccttactt ctttctttaa taatttcata 2460 agaaacggaa cagtgataat tgttatcata ggaatgagta gaagatagga ccaatgaata 2520 taatgggcta tcattccacc aatcgctgga ccgactcctt ctcccatggc tactatcgat 2580 ccaataagac caaatgcttt acccctattt tcctttggaa tatagcgcgc aactacaacc 2640 attacgagtg ctggaaatgc agctgcacca gccccttgaa taaaacgagc cataataagt 2700 aaggaaaaga aagaatggcc aacaaaccca attaccgacc cgaaacaatt tattataatt 2760 ccaaatagga gtaacctttt gatgcctaat tgatcagata gctttccata tacagctgtt 2820 ccaatggaaa aggttaacat aaaggctgtg ttcacccagt ttgtactcgc aggtggttta 2880 ttaaaatcat ttgcaatatc aggtaatgag acgttcaaaa ccatttcatt taatacgcta 2940 aaaaaagata aaatgcaaag ccaaattaaa atttggttgt gtcgtaaatt cgattgtgaa 3000 taggatgtat tcacatttca ccctccaata atgagggcag acgtagttta tagggttaat 3060 gatacgcttc cctcttttaa ttgaaccctg ttacattcat tacacttcat aattaattcc 3120 tcctaaactt gattaaaaca ttttaccaca tataaactaa gttttaaatt cagtatttca 3180 tcacttatac aacaatatgg cccgtttgtt gaactactct ttaataaaat aatttttccg 3240 ttcccaattc cacattgcaa taatagaaaa tccatcttca tcggcttttt cgtcatcatc 3300 tgtatgaatc aaatcgcctt cttctgtgtc atcaaggttt aattttttat gtatttcttt 3360 taacaaacca ccataggaga ttaacctttt acggtgtaaa ccttcctcca aatcagacaa 3420 acgtttcaaa ttcttttctt catcatcggt cataaaatcc gtatccttta caggatattt 3480 tgcagtttcg tcaattgccg attgtatatc cgatttatat ttatttttcg gtcgaatcat 3540 ttgaactttt acatttggat catagtctaa tttcattgcc tttttccaaa attgaatcca 3600 ttgtttttga ttcacgtagt tttctgtatt cttaaaataa gttggttcca cacataccaa 3660 tacatgcatg tgctgattat aagaattatc tttattattt attgtcactt ccgttgcacg 3720 cataaaacca acaagatttt tattaatttt tttatattgc atcattcggc gaaatccttg 3780 agccatatct gacaaactct tatttaattc ttcgccatca taaacatttt taactgttaa 3840 tgtgagaaac aaccaacgaa ctgttggctt ttgtttaata acttcagcaa caaccttttg 3900 tgactgaatg ccatgtttca ttgctctcct ccagttgcac attggacaaa gcctggattt 3960 acaaaaccac actcgataca actttctttc gcctgtttca cgattttgtt tatactctaa 4020 tatttcagca caatctttta ctctttcagc ctttttaaat tcaagaatat gcagaagttc 4080 aaagtaatca acattagcga ttttcttttc tctccatggt ctcacttttc cactttttgt 4140 cttgtccact aaaacccttg atttttcatc tgaataaatg ctactattag gacacataat 4200 attaaaagaa acccccatct atttagttat ttgtttggtc acttataact ttaacagatg 4260 gggtttttct gtgcaaccaa ttttaagggt tttcaatact ttaaaacaca tacataccaa 4320 cacttcaacg cacctttcag caactaaaat aaaaatgacg ttatttctat atgtatcaag 4380 ataagaaaga acaagttcaa aaccatcaaa aaaagacacc ttttcaggtg ctttttttat 4440 tttataaact cattccctga tctcgacttc gttctttttt tacctctcgg ttatgagtta 4500 gttcaaattc gttcttttta ggttctaaat cgtgtttttc ttggaattgt gctgttttat 4560 cctttacctt gtctacaaac cccttaaaaa cgtttttaaa ggcttttaag ccgtctgtac 4620 gttccttaag 4630 //