ID   T86693; SV 1; linear; mRNA; EST; HUM; 358 BP.
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AC   T86693;
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DT   31-MAR-1995 (Rel. 43, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   yd86d06.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
DE   IMAGE:115115 3', mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-358
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
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DR   MD5; fc57fe241f3ea10585967bb13a2c32df.
DR   UNILIB; 503; 262.
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CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   Insert Size: 802
CC   High quality sequence stops: 297 Source: IMAGE Consortium, LLNL
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Insert Length: 802   Std Error: 0.00
CC   Seq primer: -21m13
CC   High quality sequence stop: 297.
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FH   Key             Location/Qualifiers
FH
FT   source          1..358
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="20 week-post conception fetus"
FT                   /clone_lib="Soares fetal liver spleen 1NFLS"
FT                   /clone="IMAGE:115115"
FT                   /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
FT                   with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
FT                   1st strand cDNA was primed with a Pac I - oligo(dT) primer
FT                   [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was ligated to Eco RI adaptors
FT                   (Pharmacia), digested with Pac I and cloned into the Pac I
FT                   and Eco RI sites of the modified pT7T3 vector.  Library
FT                   went through one round of normalization. Library
FT                   constructed by Bento Soares and M.Fatima Bonaldo."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:503"
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SQ   Sequence 358 BP; 101 A; 73 C; 93 G; 88 T; 3 other;
     aggctttaat tatgacagta tttcccacac gagtcctggg atttctggaa ggggtgtgtg        60
     ggattttgcc acgcttacat cacattacca ggtggtggac aggtggggaa ggaaaaggaa       120
     gggccattat ttgtcagaag actgtttatt ggacctggga gggggtntaa cactatcaca       180
     aaggccaccc tgaactcttg atcatcagat agaccaaatc catcccctac ttgaaaagga       240
     tcttaggtag gaaaaacttg ggacacagcc atccacatct tcccaaacag aggaaaagcc       300
     atcatgcgga ctctttntgg ggtnagagct acttgtgtta aacagtttcc agggatgg         358
//