ID   T76466; SV 1; linear; mRNA; EST; PLN; 462 BP.
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AC   T76466;
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DT   25-MAR-1995 (Rel. 43, Created)
DT   31-JAN-2011 (Rel. 107, Last updated, Version 28)
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DE   11244 Lambda-PRL2 Arabidopsis thaliana cDNA clone 153A14T7, mRNA sequence.
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KW   EST.
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OS   Arabidopsis thaliana (thale cress)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
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RN   [1]
RP   1-462
RX   DOI; 10.1104/pp.106.4.1241.
RX   PUBMED; 7846151.
RA   Newman T., deBruijn F.J., Green P., Keegstra K., Kende H., McIntosh L.,
RA   Ohlrogge J., Raikhel N., Somerville S., Thomashow M., Retzel E.,
RA   Somerville C.;
RT   "Genes galore: a summary of methods for accessing results from large-scale
RT   partial sequencing of anonymous Arabidopsis cDNA clones";
RL   Plant Physiol. 106(4):1241-1255(1994).
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DR   MD5; 96fc55a609f01a1b56544e241edb640e.
DR   UNILIB; 199; 71.
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CC   On Apr 14, 1993 this sequence version replaced gi:693228.
CC   Contact: Thomas Newman
CC   MSU-DOE Plant Research Laboratory
CC   Michigan State University
CC   MSU-DOE-PRL, Michigan State University,Plant Biology Bldg.,E.
CC   Lansing,Mi
CC   Tel: 517-353-0854
CC   Fax: 517-353-9168
CC   Email: 22313tcn@ibm.cl.msu.edu
CC   Seq primer: T7 dye primer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..462
FT                   /organism="Arabidopsis thaliana"
FT                   /ecotype="Columbia"
FT                   /mol_type="mRNA"
FT                   /clone_lib="LIBEST_000071 Lambda-PRL2"
FT                   /clone="153A14T7"
FT                   /note="Vector: lambda Zip-Lox; Site_1: Sal; Site_2: Not;
FT                   Lambda PRL2 is a cDNA library derived from equal quantities
FT                   of 4 pools of mRNA. The mRNA sources were 1) 7 day
FT                   germinated etiolated seedlings; 2) tissue culture grown
FT                   roots; 3) staged plants half with 24 hour light cycle, half
FT                   on 16 hr light, 8 hour dark- rosettes; 4) same plants as 3
FT                   but aerial tissue (stems, flowers and siliques. The vector
FT                   is BRL's lambda Zip-Lox. The cDNA inserts were
FT                   directionally cloned with Sal-Not arms using oligo dT
FT                   primed cDNA."
FT                   /db_xref="taxon:3702"
FT                   /db_xref="UNILIB:199"
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SQ   Sequence 462 BP; 125 A; 97 C; 120 G; 102 T; 18 other;
     caattatctt tctnagtatt tctntaacca tttctacgtc tcaatacgaa aacggaagaa        60
     acgtaaagtc atgcagacgg tgaatattaa ggtgaagatc gactgtaacg gatgcgaacg       120
     caaaatcaag aacgctgttt cctccatcaa aggagcaaaa tcagtggaag tgaacagaaa       180
     gatgcacaaa gtaacggtga gtggctacgt tgatcccaag aaagtnctga agacagtnca       240
     gagcactggg gaaaaaggaa gctgagctgt ggccgtatgt nccatacaca atggtggctt       300
     atccatatgc cgccggngct ttcggcaaga gggcccccgc cggggttttt taaggaaagt       360
     tcgggngcag gctnaaggcg nncggnngaa agtccttccg gntaagcntc atggcccctc       420
     ttttttgggn cgggnacccc ttanggcttn ccccgttttt gg                          462
//