ID   T69498; SV 1; linear; mRNA; EST; HUM; 320 BP.
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AC   T69498;
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DT   11-MAR-1995 (Rel. 43, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 4)
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DE   yd12c06.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
DE   IMAGE:66922 5', mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-320
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
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DR   MD5; 3266a9e9bf0d5ff1560eaeab01f6e66e.
DR   UNILIB; 503; 262.
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CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   Insert Size: 999
CC   High qality sequence stops: 260 Source: IMAGE Consortium, LLNL This
CC   clone is available royalty-free through LLNL ; contact the IMAGE
CC   Consortium (info@image.llnl.gov) for further information.
CC   Insert Length: 999   Std Error: 0.00
CC   Seq primer: M13RP1
CC   High quality sequence stop: 260.
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FH   Key             Location/Qualifiers
FH
FT   source          1..320
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="20 week-post conception fetus"
FT                   /clone_lib="Soares fetal liver spleen 1NFLS"
FT                   /clone="IMAGE:66922"
FT                   /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
FT                   with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
FT                   1st strand cDNA was primed with a Pac I - oligo(dT) primer
FT                   [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was ligated to Eco RI adaptors
FT                   (Pharmacia), digested with Pac I and cloned into the Pac I
FT                   and Eco RI sites of the modified pT7T3 vector.  Library
FT                   went through one round of normalization. Library
FT                   constructed by Bento Soares and M.Fatima Bonaldo."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:503"
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SQ   Sequence 320 BP; 94 A; 63 C; 79 G; 81 T; 3 other;
     gtaaggatat tttgagttat ctggagaaaa acatctctgt acaaatgaca atagctgttg        60
     gaactcgaat gccaccgaag aacttcagcc gtggctctct agtcttcgtg tcaatatcct       120
     ttattgtttt gatgattatt tcttcagcat ggctcatatt ctacttcatt cagaagatca       180
     ggtacacaaa tgcacggcgn acagggaacc aggcgtcgtc tcgggaggat gcaggccaag       240
     gaaaggccat tcagtnaaat tgnacaacca ggggacagtt aaaggaaggg gtggacaagg       300
     gaaacttgac ccaggacttt                                                   320
//