ID   T69497; SV 1; linear; mRNA; EST; HUM; 310 BP.
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AC   T69497;
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DT   11-MAR-1995 (Rel. 43, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 4)
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DE   yd12c05.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
DE   IMAGE:66920 5', mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-310
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
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DR   MD5; 5ac4d627a6f510ffcaa9d4765a4484a2.
DR   UNILIB; 503; 262.
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CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   Insert Size: 1271
CC   High qality sequence stops: 184 Source: IMAGE Consortium, LLNL This
CC   clone is available royalty-free through LLNL ; contact the IMAGE
CC   Consortium (info@image.llnl.gov) for further information.
CC   Insert Length: 1271   Std Error: 0.00
CC   Seq primer: M13RP1
CC   High quality sequence stop: 184.
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FH   Key             Location/Qualifiers
FH
FT   source          1..310
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="20 week-post conception fetus"
FT                   /clone_lib="Soares fetal liver spleen 1NFLS"
FT                   /clone="IMAGE:66920"
FT                   /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
FT                   with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
FT                   1st strand cDNA was primed with a Pac I - oligo(dT) primer
FT                   [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was ligated to Eco RI adaptors
FT                   (Pharmacia), digested with Pac I and cloned into the Pac I
FT                   and Eco RI sites of the modified pT7T3 vector.  Library
FT                   went through one round of normalization. Library
FT                   constructed by Bento Soares and M.Fatima Bonaldo."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:503"
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SQ   Sequence 310 BP; 38 A; 94 C; 86 G; 85 T; 7 other;
     tgaaccccac ctcgtgtcgt catgatctga gggctctngc aagaaatcct agtttcgtcc        60
     tgtcttccct gggcttcact gctgtggcct ttgtcacggg ctccctggct ctgtgggctc       120
     cggcattcct nctgcgttcc cgcgtggttc cttggggaga ccccaccctg ccttcccgga       180
     gactnctgct tcttcctctg gacagtcttc atctttggga ctncattcac ctgactgnac       240
     cgggagtcct gggggtgtgg ggcctggggt gtgggagatn caagccgncg gttctcggca       300
     acttttcaaa                                                              310
//