ID   T67841; SV 1; linear; mRNA; EST; HUM; 454 BP.
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AC   T67841;
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DT   10-MAR-1995 (Rel. 43, Created)
DT   31-JAN-2011 (Rel. 107, Last updated, Version 5)
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DE   yc27f07.r1 Stratagene liver (#937224) Homo sapiens cDNA clone IMAGE:81925
DE   5' similar to gb:K02765 COMPLEMENT C3 PRECURSOR (HUMAN), mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-454
RX   PUBMED; 8889549.
RA   Hillier L., Lennon G., Becker M., Bonaldo M.F., Chiapelli B., Chissoe S.,
RA   Dietrich N., DuBuque T., Favello A., Gish W., Hawkins M., Hultman M.,
RA   Kucaba T., Lacy M., Le M., Le N., Mardis E., Moore B., Morris M.,
RA   Parsons J., Prange C., Rifkin L., Rohlfing T., Schellenberg K.,
RA   Soares M.B., Tan F., Thierry-Meg J., Trevaskis E., Underwood K.,
RA   Wohldmann P., Waterston R., Wilson R., Marra M.;
RT   "Generation and analysis of 280,000 human expressed sequence tags";
RL   Genome Res. 6(9):807-828(1996).
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DR   MD5; 0f11c60ce33fd05033a2ddff4f0111a1.
DR   UNILIB; 501; 252.
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CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   Insert Size: 61
CC   High qality sequence stops: 326 Source: IMAGE Consortium, LLNL This
CC   clone is available royalty-free through LLNL ; contact the IMAGE
CC   Consortium (info@image.llnl.gov) for further information.
CC   Insert Length: 61   Std Error: 0.00
CC   Seq primer: M13RP1
CC   High quality sequence stop: 326.
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FH   Key             Location/Qualifiers
FH
FT   source          1..454
FT                   /organism="Homo sapiens"
FT                   /lab_host="SOLR cells (kanamycin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="49 years old"
FT                   /clone_lib="LIBEST_000252 Stratagene liver (#937224)"
FT                   /clone="IMAGE:81925"
FT                   /note="Organ: liver; Vector: pBluescript SK; Site_1: EcoRI;
FT                   Site_2: XhoI; Cloned unidirectionally. Primer: Oligo dT.
FT                   Hepatectomy from normal male caucasian. Average insert
FT                   size: 1.1 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5'
FT                   GAATTCGGCACGAG 3' ~3' adaptor sequence: 5'
FT                   CTCGAGTTTTTTTTTTTTTTTTTT 3'"
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:501"
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SQ   Sequence 454 BP; 124 A; 105 C; 125 G; 97 T; 3 other;
     caaagttcac caatacttta atgtagagct tatccagcct ggagcagtca aggtctacgc        60
     ctattacaac ctggaggaaa gctgtacccg gttctaccat ccggaaaagg aggatggaaa       120
     gctgaacaag ctctgccgtg atgaactgtg ccgctgtgct gaggagaatt gcttcataca       180
     aaagtcggat gacaaggtca ccctgggaag aacggctgga caaggcctgt gagccaggga       240
     gtggactatg tgtacaagac ccgactggtc aaggttcagc tgtccaatga ctttgacgag       300
     tacatcatgg ccattgagca gaccatcaag tcaggcttcg gatgaggtgc aggttnggac       360
     agcagcgcac gtttcattca gccccatcca agttgcagag aagccctgaa gcttggagga       420
     gaaggaaaca ttacctcatn ttggggtctn ttcc                                   454
//