ID   T04434; SV 1; linear; mRNA; EST; PLN; 400 BP.
XX
AC   T04434;
XX
DT   30-AUG-1993 (Rel. 36, Created)
DT   31-JAN-2011 (Rel. 107, Last updated, Version 26)
XX
DE   481 Lambda-PRL2 Arabidopsis thaliana cDNA clone 40F9T7, mRNA sequence.
XX
KW   EST.
XX
OS   Arabidopsis thaliana (thale cress)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
XX
RN   [1]
RP   1-400
RX   DOI; 10.1104/pp.106.4.1241.
RX   PUBMED; 7846151.
RA   Newman T., deBruijn F.J., Green P., Keegstra K., Kende H., McIntosh L.,
RA   Ohlrogge J., Raikhel N., Somerville S., Thomashow M., Retzel E.,
RA   Somerville C.;
RT   "Genes galore: a summary of methods for accessing results from large-scale
RT   partial sequencing of anonymous Arabidopsis cDNA clones";
RL   Plant Physiol. 106(4):1241-1255(1994).
XX
DR   MD5; 1286e4ff66a0c1f6532e8889ef117cb1.
DR   UNILIB; 199; 71.
XX
CC   Contact: Thomas Newman
CC   MSU-DOE Plant Research Laboratory
CC   Michigan State University
CC   MSU-DOE-PRL, Michigan State University,Plant Biology Bldg.,E.
CC   Lansing,Mi
CC   Tel: 517-353-0854
CC   Fax: 517-353-9168
CC   Email: 22313tcn@ibm.cl.msu.edu
CC   Seq primer: T7 dye primer.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..400
FT                   /organism="Arabidopsis thaliana"
FT                   /ecotype="Columbia"
FT                   /mol_type="mRNA"
FT                   /clone_lib="LIBEST_000071 Lambda-PRL2"
FT                   /clone="40F9T7"
FT                   /note="Vector: lambda Zip-Lox; Site_1: Sal; Site_2: Not;
FT                   Lambda PRL2 is a cDNA library derived from equal quantities
FT                   of 4 pools of mRNA. The mRNA sources were 1) 7 day
FT                   germinated etiolated seedlings; 2) tissue culture grown
FT                   roots; 3) staged plants half with 24 hour light cycle, half
FT                   on 16 hr light, 8 hour dark- rosettes; 4) same plants as 3
FT                   but aerial tissue (stems, flowers and siliques. The vector
FT                   is BRL's lambda Zip-Lox. The cDNA inserts were
FT                   directionally cloned with Sal-Not arms using oligo dT
FT                   primed cDNA."
FT                   /db_xref="taxon:3702"
FT                   /db_xref="UNILIB:199"
XX
SQ   Sequence 400 BP; 90 A; 96 C; 93 G; 119 T; 2 other;
     ggagcgaatc tgatctccgg tcgtcagctt cgtccaattc tncttctcga ttcgtcggcg        60
     atcaacggag gagagaaaag agaaattctc aaaccggtta aagccgccgc tgctgaaggt       120
     ggagataccg ctggggatgc taaagttgga ttcctcgcca agtatccatg gctagtcact       180
     ggattcttct tcttcatgtg gtacttcttg aatgtgattt tcaacatcct taacaagaag       240
     atctataatt acttccctta tccctatttt gtatcggtga tacacttgtt cgtgggagtt       300
     gtttactgct tgancagctg gtcccgtggg tcttcctaaa cgtgccccga ttgactcgaa       360
     cctcctcaag gtattgataa ccagtcgcag tctgtcacgg                             400
//