ID   R59848; SV 1; linear; mRNA; EST; HUM; 325 BP.
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AC   R59848;
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DT   29-MAY-1995 (Rel. 43, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 4)
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DE   yh11h06.r1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE:43109 5',
DE   mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-325
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
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DR   MD5; 7480b23078cc4b9c96641207606692d1.
DR   UNILIB; 504; 263.
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CC   On May 18, 1995 this sequence version replaced gi:811167.
CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   High quality sequence stops: 135
CC   Source: IMAGE Consortium, LLNL
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Seq primer: M13RP1
CC   High quality sequence stop: 135.
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FH   Key             Location/Qualifiers
FH
FT   source          1..325
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="female"
FT                   /dev_stage="73 days post natal"
FT                   /clone_lib="Soares infant brain 1NIB"
FT                   /clone="IMAGE:43109"
FT                   /note="Organ: whole brain; Vector: Lafmid BA; Site_1: Not
FT                   I; Site_2: Hind III; 1st strand cDNA was primed with a Not
FT                   I - oligo(dT) primer [5'
FT                   AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT 3'];
FT                   double-stranded cDNA was ligated to Hind III adaptors
FT                   (Pharmacia), digested with Not I and directionally cloned
FT                   into the Not I and Hind III sites of the Lafmid BA vector.
FT                   Library went through one round of normalization. Library
FT                   constructed by Bento Soares and M.Fatima Bonaldo."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:504"
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SQ   Sequence 325 BP; 50 A; 102 C; 112 G; 57 T; 4 other;
     cagaggagca gtgtccggcc agagcgcgcg cgcagcttgg gagctaggca nggcctggcg        60
     ctggggtcgg gctngcgtca tgcgtgccac gtctcctcta cgcgccggac cctgggatgc       120
     tcttcggccg catcccgctg cgctacgcca tactgatgca gatgcgcttc gatggacgcc       180
     tgggcttccc cggcggattc gtggacacgc aggacagaag cctagaggac gggctgaacc       240
     gcgagctgcg nagggagctg ggcaaagggc ttgccgtttc cgcgttgagc caacttgact       300
     accgcanctt cccacgttcg ggttc                                             325
//