ID   R45668; SV 1; linear; mRNA; EST; HUM; 379 BP.
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AC   R45668;
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DT   13-MAY-1995 (Rel. 43, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 3)
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DE   yg44g12.s1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE:35384 3'
DE   similar to contains L1 repetitive element ;, mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-379
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
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DR   MD5; c8501362086231fa5b1324bd4b027f66.
DR   UNILIB; 504; 263.
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CC   On May 9, 1995 this sequence version replaced gi:804392.
CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   Insert Size: 1901
CC   High quality sequence stops: 275 Source: IMAGE Consortium, LLNL
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Insert Length: 1901   Std Error: 0.00
CC   Seq primer: Promega -21m13
CC   High quality sequence stop: 275.
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FH   Key             Location/Qualifiers
FH
FT   source          1..379
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="female"
FT                   /dev_stage="73 days post natal"
FT                   /clone_lib="Soares infant brain 1NIB"
FT                   /clone="IMAGE:35384"
FT                   /note="Organ: whole brain; Vector: Lafmid BA; Site_1: Not
FT                   I; Site_2: Hind III; 1st strand cDNA was primed with a Not
FT                   I - oligo(dT) primer [5'
FT                   AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT 3'];
FT                   double-stranded cDNA was ligated to Hind III adaptors
FT                   (Pharmacia), digested with Not I and directionally cloned
FT                   into the Not I and Hind III sites of the Lafmid BA vector.
FT                   Library went through one round of normalization. Library
FT                   constructed by Bento Soares and M.Fatima Bonaldo."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:504"
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SQ   Sequence 379 BP; 66 A; 64 C; 75 G; 172 T; 2 other;
     tttttttttt gaagggtttt tcatgtctct gtctccttca gttccacttt gatcttagtc        60
     attttttttt ctcttctgct aacttttgga tttgtttgct cttgcttctc tagttctttt       120
     agttgtgatg ttagggtgtc aatttgagat atgtctagct ttatgatgtg ggcatttagt       180
     gctataaatt tacctcttaa cactgcttta gttgcctccc agagattctg ggnacattgt       240
     ctctttgttc tcattggntt caaagaggct tcttgatttc tgccttaatt ttattattta       300
     cccaggaagt cactcagggg gcacgttatt caatatccat gtagttgtgt gggttttgag       360
     gtgaggtttc ttaatcttg                                                    379
//