ID   R36730; SV 1; linear; mRNA; EST; HUM; 476 BP.
XX
AC   R36730;
XX
DT   05-MAY-1995 (Rel. 43, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
XX
DE   yg06h04.r9 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE:31588 5',
DE   mRNA sequence.
XX
KW   EST.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-476
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
XX
DR   MD5; d84734ac992704a467ec924b9ecedbb9.
DR   UNILIB; 504; 263.
XX
CC   On Sep 21, 1992 this sequence version replaced gi:276239.
CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   Insert Size: 2606
CC   High quality sequence stops: 333 Source: IMAGE Consortium, LLNL
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Insert Length: 2606   Std Error: 0.00
CC   Seq primer: M13RP1
CC   High quality sequence stop: 333.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..476
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="female"
FT                   /dev_stage="73 days post natal"
FT                   /clone_lib="Soares infant brain 1NIB"
FT                   /clone="IMAGE:31588"
FT                   /note="Organ: whole brain; Vector: Lafmid BA; Site_1: Not
FT                   I; Site_2: Hind III; 1st strand cDNA was primed with a Not
FT                   I - oligo(dT) primer [5'
FT                   AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT 3'];
FT                   double-stranded cDNA was ligated to Hind III adaptors
FT                   (Pharmacia), digested with Not I and directionally cloned
FT                   into the Not I and Hind III sites of the Lafmid BA vector.
FT                   Library went through one round of normalization. Library
FT                   constructed by Bento Soares and M.Fatima Bonaldo."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:504"
XX
SQ   Sequence 476 BP; 123 A; 91 C; 118 G; 138 T; 6 other;
     tagacagagg gtccttattt gcttgtccgt ggtgtctatg tgggcctatt gagggaatgg        60
     tagttacttg aaggttcaca tatgtaagcg tgtggaccca gcacagtctg tgacctgatt       120
     ccagatcctc aagagtggag aaggaaagaa atatgatgat ttcctatgct tgaccctaac       180
     ccaaccccaa aactgctagg gattcaacag tgagtaagat gtatgcaata agagaacaga       240
     taacttcttc aacaatgtgc atgccgtttg tgtaggatgt tcaggaactt acatatgtgt       300
     gaatatcact cccaccttta cacacggcac acacactggc aatcacatgg gcatttaaaa       360
     attntattgt gtatggggaa cttgggcgag gttcagttca gggaccnggg gagctgttan       420
     tcttaggcct ttttttgcca cttncttggc gtttgttgtt ntaccattgg antggg           476
//