ID   R24148; SV 1; linear; mRNA; EST; HUM; 301 BP.
XX
AC   R24148;
XX
DT   23-APR-1995 (Rel. 43, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
XX
DE   yh29b11.s1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE:131133 3',
DE   mRNA sequence.
XX
KW   EST.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-301
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
XX
DR   MD5; dc706444b2c4ca665911bd852a876bbd.
DR   UNILIB; 551; 271.
XX
CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   High quality sequence stops: 257
CC   Source: IMAGE Consortium, LLNL
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Seq primer: -21m13
CC   High quality sequence stop: 257.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..301
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="Female"
FT                   /dev_stage="placenta obtained at birth (full term)"
FT                   /clone_lib="Soares placenta Nb2HP"
FT                   /clone="IMAGE:131133"
FT                   /note="Organ: placenta; Vector: pT7T3D (Pharmacia) with a
FT                   modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
FT                   strand cDNA was primed with a Not I - oligo(dT) primer [5'
FT                   AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was ligated to Eco RI adaptors
FT                   (Pharmacia), digested with Not I and cloned into the Not I
FT                   and Eco RI sites of the modified pT7T3 vector. Library went
FT                   through one round of normalization. Library constructed by
FT                   Bento Soares and M.Fatima Bonaldo.  "
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:551"
XX
SQ   Sequence 301 BP; 97 A; 49 C; 64 G; 91 T; 0 other;
     taaatcttta ttgaattttt attgtaacag caatgcaata ttagcaaata gagagaaata        60
     tagagtgaag agaatacagc aataaagtta gaaggagggg taggggcaat ggggttcagt       120
     ggtaggagga gggctactga caggggtaaa agcaagggtt tttatttatc acttttataa       180
     cttccatgaa aatttaccat ccatcattga caagttcatt ctagcagtaa tttatcagag       240
     gtcactcatc ctctattaaa ttattatcct aatccccctt ggaccttttc cccccggggg       300
     t                                                                       301
//