ID   ON834735; SV 1; linear; genomic DNA; STD; INV; 658 BP.
XX
AC   ON834735;
XX
DT   30-JUN-2022 (Rel. 144, Created)
DT   05-AUG-2022 (Rel. 144, Last updated, Version 2)
XX
DE   Diamesa leona isolate EAM139 cytochrome c oxidase subunit I (COX1) gene,
DE   partial cds; mitochondrial.
XX
KW   .
XX
OS   Diamesa leona
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Chironomoidea; Chironomidae;
OC   Diamesinae; Diamesa.
OG   Mitochondrion
XX
RN   [1]
RP   1-658
RA   Makarchenko E.A., Semenchenko A.A., Palatov D.M.;
RT   "Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae),
RT   with description and DNA barcoding of known species. II. Subgroups davisi
RT   and leona";
RL   Unpublished.
XX
RN   [2]
RP   1-658
RA   Makarchenko E.A., Semenchenko A.A., Palatov D.M.;
RT   ;
RL   Submitted (22-JUN-2022) to the INSDC.
RL   Laboratory of Ecology and Evolutionary Biology of Aquatic Organisms, Far
RL   Eastern Federal University, Suchanova 8, Vladivostok, Primorskii territory
RL   690922, Russia
XX
DR   MD5; 36494f60c56822a10bc04a359b2371a6.
XX
CC   ##Assembly-Data-START##
CC   Sequencing Technology :: Sanger dideoxy sequencing
CC   ##Assembly-Data-END##
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..658
FT                   /organism="Diamesa leona"
FT                   /organelle="mitochondrion"
FT                   /isolate="EAM139"
FT                   /mol_type="genomic DNA"
FT                   /country="Russia: Primorye Territory, Khasanskii district"
FT                   /lat_lon="43.1 N 131.56 E"
FT                   /isolation_source="Kedrovaya river, Kedrovyi Nature
FT                   Reserve"
FT                   /collected_by="Makarchenko, E.A."
FT                   /collection_date="19-Mar-2016"
FT                   /identified_by="Makarchenko, E.A."
FT                   /sex="female"
FT                   /dev_stage="Imago"
FT                   /PCR_primers="fwd_name: LCO1490,
FT                   fwd_seq:ggtcaacaaatcataaagatattgg, rev_name: HCO2198,
FT                   rev_seq:taaacttcagggtgaccaaaaaatca"
FT                   /db_xref="taxon:2954860"
FT   gene            <1..>658
FT                   /gene="COX1"
FT   CDS             <1..>658
FT                   /codon_start=2
FT                   /transl_table=5
FT                   /gene="COX1"
FT                   /product="cytochrome c oxidase subunit I"
FT                   /protein_id="USN34976.1"
FT                   /translation="TLYFIFGAWAGMVGTSLSILIRAELGHPGSLIGDDQIYNVIVTAH
FT                   AFVMIFFMVMPIMIGGFGNWLVPLMLGAPDMAFPRMNNMSFWMLPPSLTLLLASSIVEN
FT                   GAGTGWTVYPPLSSGIAHAGASVDLAIFSLHLAGISSILGAVNFITTVINMRSSGITLD
FT                   RMPLFVWSVVITAVLLLLSLPVLAGAITMLLTDRNLNTSFFDPAGGGDPILYQHLF"
XX
SQ   Sequence 658 BP; 171 A; 111 C; 110 G; 266 T; 0 other;
     aactttatat tttatttttg gagcttgagc tggaatagta ggtacttccc ttagtatctt        60
     aattcgagct gagcttggac atcccggttc attaattgga gatgatcaaa tttataatgt       120
     aattgtaact gctcatgctt ttgttataat tttttttata gtaataccta ttatgattgg       180
     aggttttgga aattgattag ttccccttat actaggggct cctgatatag cttttcctcg       240
     aataaataac ataagttttt gaatactccc accttctctt acacttcttc ttgctagttc       300
     aattgttgaa aacggagctg gtacaggttg aactgtttac ccccctctct catcaggaat       360
     tgctcatgct ggagcatcag ttgatttagc tattttttca ttacatttag ctggaatttc       420
     ttctatttta ggagctgtaa attttattac aacagttatt aatatgcgtt ctagtggaat       480
     tactttagat cgaatacctt tatttgtgtg atctgtagta attacagcag ttcttctatt       540
     actttcttta ccagtattag ctggagctat tacaatactt cttacagatc gaaatttaaa       600
     tacatcgttt ttcgatcctg ctggaggagg ggatcctatt ctttatcaac atttattt         658
//