ID   N39176; SV 1; linear; mRNA; EST; HUM; 533 BP.
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AC   N39176;
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DT   25-JAN-1996 (Rel. 46, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   yv26c01.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
DE   IMAGE:243840 3' similar to gb:M57298 G25K GTP-BINDING PROTEIN, PLACENTAL
DE   ISOFORM (HUMAN);, mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-533
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
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DR   MD5; e68f1de0bbaff59ca1675debae91fc8d.
DR   UNILIB; 503; 262.
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CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   High quality sequence stops: 331
CC   Source: IMAGE Consortium, LLNL
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Seq primer: m13 -40 forward
CC   High quality sequence stop: 331.
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FH   Key             Location/Qualifiers
FH
FT   source          1..533
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="20 week-post conception fetus"
FT                   /clone_lib="Soares fetal liver spleen 1NFLS"
FT                   /clone="IMAGE:243840"
FT                   /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
FT                   with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
FT                   1st strand cDNA was primed with a Pac I - oligo(dT) primer
FT                   [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was ligated to Eco RI adaptors
FT                   (Pharmacia), digested with Pac I and cloned into the Pac I
FT                   and Eco RI sites of the modified pT7T3 vector.  Library
FT                   went through one round of normalization. Library
FT                   constructed by Bento Soares and M.Fatima Bonaldo."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:503"
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SQ   Sequence 533 BP; 138 A; 113 C; 127 G; 148 T; 7 other;
     gaaacaacaa aaaaaaatag tactaatcac ttttctgaca atacaattac tcaaaattaa        60
     ctagtactgg gagggnnaag gggggggcca tacctatggg ccttgtctca cacgagtgca       120
     tgtgggtagg tgcagggcat ttgtcattat tgcaaaaacg aattttaatt tttaatcttt       180
     agtttgattt aaacattgct tttagtatga tgccgacacc agctgtgcag aaagggctct       240
     ggagagatgt tcatagcagc acacacctgc ggctcttctt cagttctgga ggctccaggg       300
     cagccaatat tgcttcgtca aatacattct ttaggccttt ctgtgtaagt gcagaacact       360
     ccacatactt gacagccttc aggtcacggg ccagnctttt tcagcagtct ctgggagtga       420
     tangcctccg tttgttcctg ggcnagtttc nccatagtag anggggtcat ctctgagatc       480
     catttgagtc ccacaagcca gaaggaggtc cttggacagt ggggaggtat ctc              533
//