ID   MG136431; SV 1; linear; other DNA; STD; SYN; 1105 BP.
XX
AC   MG136431;
XX
DT   20-NOV-2017 (Rel. 134, Created)
DT   24-FEB-2018 (Rel. 135, Last updated, Version 2)
XX
DE   Synthetic construct Xenopus tropicalis clone IMAGE:7633380 hypothetical
DE   protein (LOC496786) gene, encodes complete protein.
XX
KW   .
XX
OS   synthetic construct
OC   other sequences; artificial sequences.
XX
OS   Xenopus tropicalis (tropical clawed frog)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
XX
RN   [1]
RP   1-1105
RA   Balcha D., Shen Y., Bian W., De Ridder D., Hao T., Zorn A.,
RA   Stukenberg P.T., Gilchrist M.J., Hill D.E.;
RT   "Xenopus tropicalis ORFeome version 1.0 library";
RL   Unpublished.
XX
RN   [2]
RP   1-1105
RA   Balcha D., Shen Y., Bian W., De Ridder D., Hao T., Zorn A.,
RA   Stukenberg P.T., Gilchrist M.J., Hill D.E.;
RT   ;
RL   Submitted (10-OCT-2017) to the INSDC.
RL   Cancer Biology, Dana Farber Cancer Institute, 450 Brookline Avenue, Boston,
RL   MA 02215, USA
XX
DR   MD5; d24429e0613cf09e066ecada48890150.
XX
CC   ORF Sequence Tag (OST) of Gateway Entry construct. Each cloned ORF
CC   results from a PCR reaction using an XGC full-length cDNA as
CC   template DNA and ORF specific primers.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1105
FT                   /organism="synthetic construct"
FT                   /focus
FT                   /lab_host="E. coli"
FT                   /mol_type="other DNA"
FT                   /clone_lib="NIH_XGC_tropTad5"
FT                   /clone="IMAGE:7633380"
FT                   /note="Vector:pDONR223; The ORFs were PCR amplified from
FT                   the XGC (Xenopus Gene Collection) and cloned by
FT                   recombinational Gateway cloning into pDONR223 Donor vector.
FT                   References: XGC (Xenopus Gene Collection) NIH initiative,
FT                   web site: http://xgc.nci.nih.gov/ and XenBase:
FT                   http://www.xenbase.org/"
FT                   /db_xref="taxon:32630"
FT   source          20..1087
FT                   /organism="Xenopus tropicalis"
FT                   /mol_type="other DNA"
FT                   /db_xref="taxon:8364"
FT   misc_feature    1..19
FT                   /note="attL1 site"
FT   gene            20..>1087
FT                   /gene="LOC496786"
FT   CDS             20..>1087
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="LOC496786"
FT                   /product="hypothetical protein"
FT                   /note="missing stop codon"
FT                   /protein_id="ATO97783.1"
FT                   /translation="MAAEESSSSPGGITPLQQILASGTGALLTSLFVTPLDVVKIRLQA
FT                   QRKPLSKVLSVKPLPWALPMRHPKWRCFLYCNGLMDHLFVCQHATACSTWYRAPTYFNG
FT                   TLDAFVKITRHEGLTSLWSGLPPTLVMAVPATIIYFTCYDQLRDFLCYGLGYHGSHIPL
FT                   IAGALARLGAVTVISPLELIRTKMQSRQLSYMELGVCLRSAVSQDGWLSLWKGWGPTVL
FT                   RDVPFSALYWFNYELVKKKMSNTKAAVESPFLVSFSAGAVSGAVAAVLTLPFDVVKTQR
FT                   QIELGNLELGPSRKQRSSTWGAMRRIRAESGTRGLFAGFLPRVIKVAPACAIMISSYEF
FT                   GKNFFQQQNKIKQQNV"
FT   misc_feature    1088..1105
FT                   /note="attL2 site"
XX
SQ   Sequence 1105 BP; 266 A; 278 C; 264 G; 297 T; 0 other;
     gtacaaaaaa gttggcacca tggctgcaga ggaatccagc tcatccccag gtgggatcac        60
     tcccctgcag cagattctgg cctctggtac tggagctctc ctcacctccc tctttgtgac       120
     ccctctggat gttgtgaaga tcagattgca ggcacagagg aaacccctct ctaaagtttt       180
     gtctgtaaag cctcttccct gggcacttcc catgcgccat cccaaatggc gctgcttcct       240
     ctattgtaat ggactgatgg atcatctttt cgtgtgccaa catgccacgg cttgcagcac       300
     ctggtatcga gcaccaacat actttaatgg cacactggat gcatttgtaa aaattacacg       360
     gcatgaaggt ttaacatcac tatggagtgg actgcccccg acattagtga tggctgtacc       420
     tgctaccatc atatatttca catgttacga tcagttgcga gatttccttt gttatgggct       480
     cggctaccat ggcagccaca ttcccctcat agctggagct cttgctcgct tgggagccgt       540
     tacagttatt agcccacttg agctgattcg cactaagatg cagtctcgcc agctttccta       600
     catggagttg ggtgtctgtc tgcggtctgc agtttctcag gatggatggc tgtctctttg       660
     gaaaggatgg ggaccaacag tgctgagaga tgtcccattc tctgctctgt actggtttaa       720
     ttatgaactt gtgaagaaga agatgagcaa caccaaagca gctgtagaga gccccttcct       780
     agtcagcttc agcgctggtg ctgtgtctgg agcggttgct gcagtactta ctctgccatt       840
     tgatgtagtg aagacccaga gacagattga gttgggaaac ctggaactag gtccatccag       900
     aaaacagaga tcttccacct ggggggccat gcgtagaatt cgagcagaat ctggaactag       960
     aggcctgttt gcaggttttc tcccgcgagt cattaaagtt gctcctgcct gcgccataat      1020
     gatcagctca tatgagtttg gaaagaactt cttccaacaa cagaacaaga taaaacaaca      1080
     aaacgtattg ccaactttct tgtac                                            1105
//