ID   KJ414467; SV 1; linear; genomic DNA; STD; INV; 1570 BP.
XX
AC   KJ414467;
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DT   16-JAN-2015 (Rel. 123, Created)
DT   16-JAN-2015 (Rel. 123, Last updated, Version 1)
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DE   Robbea hypermnestra isolate RBhyp42f 18S ribosomal RNA gene, partial
DE   sequence.
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KW   GSC:MIxS; MIMARKS:3.0.
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OS   Robbea hypermnestra
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Desmodorida;
OC   Desmodorina; Desmodoroidea; Desmodoridae; Stilbonematinae; Robbea.
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RN   [1]
RP   1-1570
RA   Ott J.A., Gruber-Vodicka H.R., Leisch N., Zimmermann J.;
RT   "Phylogenetic confirmation of the genus Robbea (Nematoda: Desmodoridae,
RT   Stilbonematinae) with the description of three new species";
RL   System. Biodivers. 2014:1-22(2014).
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RN   [2]
RP   1-1570
RA   Ott J.A., Gruber-Vodicka H.R., Leisch N., Zimmermann J.;
RT   ;
RL   Submitted (06-FEB-2014) to the INSDC.
RL   Symbiosis, Max Planck Institute for Marine Microbiology, Celsiusstr. 1,
RL   Bremen 28359, Germany
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DR   MD5; 784cde88d5e85266c5f0dfa9d9a4e52a.
DR   SILVA-SSU; KJ414467.
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CC   ##MIMARKS:3.0-Data-START##
CC   investigation_type  :: mimarks-survey
CC   collection_date     :: 2013-04-22
CC   depth               :: 0.2
CC   biome               :: marine biome
CC   feature             :: tropical island
CC   material            :: coral reef sediment
CC   geo_loc_name        :: Belize, Carrie Bow Cay
CC   lat_lon             :: 16.81306 N 88.08278 W
CC   lib_reads_seqd      :: 0
CC   lib_size            :: 0
CC   nucl_acid_ext       :: Gene releaser
CC   pcr_cond            :: initial denaturation: 96degreesC_5min,
CC                          denaturation: 96degreesC_1min, annealing:
CC                          42degreesC_1.5min, elongation:
CC                          72degreesC_2min, final elongation:
CC                          72degreesC_10min, 35cycles, 5Prime DNA
CC                          Polymerase
CC   pcr_primers         :: Fwd:GGTTGATYCTGCCAGT Rev:GGTTCACCTACGRAAAC
CC   rel_to_oxygen       :: microoxic
CC   samp_collect_device :: bucket
CC   samp_mat_process    :: stirring sediment, decanting worms in sieve
CC   seq_quality_check   :: manual
CC   seq_meth            :: Sanger
CC   target_gene         :: 18S rRNA
CC   env_package         :: host-associated
CC   depth               :: 0.2
CC   host_color          :: white
CC   host_common_name    :: Stilbonematine nematode
CC   host_shape          :: worm
CC   host_subject_id     :: Robbea cf. "hypermnestra" 42f, female
CC   temp                :: 27degreesC
CC   created with        :: CDinFusion
CC   project_name        :: Three new species of Robbea
CC   ##MIMARKS:3.0-Data-END##
CC   ##Assembly-Data-START##
CC   Assembly Method       :: Sequencher v. 4.6
CC   Sequencing Technology :: Sanger dideoxy sequencing
CC   ##Assembly-Data-END##
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FH   Key             Location/Qualifiers
FH
FT   source          1..1570
FT                   /organism="Robbea hypermnestra"
FT                   /isolate="RBhyp42f"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:74813"
FT   rRNA            <1..>1570
FT                   /product="18S ribosomal RNA"
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SQ   Sequence 1570 BP; 408 A; 319 C; 407 G; 419 T; 17 other;
     atgtctaagc atgagccgaa ttatggtgaa gccgcgaatg gctcattaca acagctgnnn        60
     nnnnnnnatc ttactttact tggataactg tagtaattnn agagctnata catgcaattc       120
     agctctgacc tcatgaggaa ggagtgcatt tgttagacca aaaccaatca gactttggtc       180
     tgttgtttgg tgactctgaa taactctgct gatcgcatgg tccttgtact ggcgacatat       240
     ctttcaagtg tctgccctat caactttcga tggtagttta tgtggctacc atggttgtaa       300
     cgggtaacgg agaataaggg ttcgactccg gagagggagc ctgagaaatg gctaccacat       360
     ccaaggaagg cagcaggcgc gcaaattacc cactctcaga tcgaggaggt agtgacgaaa       420
     aataacgaga tggttctctt tgaggcccat catcggaatg ggtacaattt aaacccttta       480
     atgaggatct attggagggc aagtctggtg ccagcagccg cggtaattcc agctccaata       540
     gcatatacca attctgctgc tgttaaaaag ctcgtagttg gatcttttct gaggatgcag       600
     tctgtctctg tgacatgtac tgtattccaa agattcaccc actggtctgg tcatggtcct       660
     cttgactgag tgctttggct ggctagtatg tttactttga aaaaattaga gtgctcaaaa       720
     caggcgctcc agcctgcata gtggtgcatg gaataatgga ataggatctc agttctattt       780
     tgttggtttt aagagcgtga gataatgatt aagagggaca gacgggggca ttcgtattgc       840
     ggcgttagag gtgaaattct tggatcgccg caagacgaac aactgcgaaa gcatttgcca       900
     agaatgtttt cattaatcaa gaacgaaagt tagaggttcg aaggcgatca gataccgccc       960
     tagttctaac cgtaaacgat gccaattagc gatctgctgg tgttaatcat gacctagcag      1020
     gcagctttcc ggaaacgaaa atctttcggt tcagggggaa gtatggttgc aaagctgaaa      1080
     cttaaaggaa ttgacggaag ggcaccacca ggcgtggagc ctgcggctta atttgactca      1140
     acacgggaaa actcacccgg cccggacact gtaaggattg acagattgat agctctttct      1200
     tgattcagtg ggtggtggtg catggccgtt cttagttggt ggagcgattt gtctggttaa      1260
     ttccgataac gaacgagact ctagcctgct aaatagtctg cggatcgcac tgtctgcagt      1320
     acttcttaga gggacaagcg gcatttagcc gcacgaaatt gagcaataac aggtctgtga      1380
     tgcccttaga tgtccggggc tgcacgcgcg ctacactgaa ggaatcaata tgcttgtttc      1440
     cttgtccgag aggactgggt aaccctttga aactccttcg tgattgggat agggaattgc      1500
     nnnnatttcc cttgaacgag gaatgcctag taagtgtggg tcataagctc acgttgatta      1560
     cgtccctgcc                                                             1570
//