ID J01347; SV 1; circular; genomic DNA; STD; FUN; 6318 BP. XX AC J01347; L00321-L00324; M10185; M11111; M11593; M14239-M14245; AC M14253-M14259; M14591-M14598; V01323; XX DT 29-AUG-1992 (Rel. 33, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 9) XX DE Saccharomyces cerevisiae 2 micron circle plasmid, complete sequence. XX KW circular; complete genome; d protein; DNA-binding protein; plasmid; KW protein FLP; recombinase; Rep-1 protein; Rep-2 protein; repeat region. XX OS Saccharomyces cerevisiae (baker's yeast) OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OG 2 micron circle plasmid XX RN [1] RP 1-1022 RX DOI; 10.1093/nar/7.2.361. RX PUBMED; 386282. RA Hindley J., Phear G.A.; RT "Sequence of 1019 nucleotides encompassing one of the inverted repeats from RT the yeast 2 micrometer plasmid"; RL Nucleic Acids Res. 7(2):361-375(1979). XX RN [2] RP 1-6318 RX DOI; 10.1038/286860a0. RX PUBMED; 6251374. RA Hartley J.L., Donelson J.E.; RT "Nucleotide sequence of the yeast plasmid"; RL Nature 286(5776):860-865(1980). XX RN [3] RP 3891-3990 RX DOI; 10.1016/0092-8674(82)90107-6. RX PUBMED; 6286142. RA Broach J.R., Guarascio V.R., Jayaram M.; RT "Recombination within the yeast plasmid 2mu circle is site-specific"; RL Cell 29(1):227-234(1982). XX RN [4] RP 3881-4020 RX PUBMED; 6397322. RA McLeod M., Volkert F., Broach J.; RT "Components of the site-specific recombination system encoded by the yeast RT plasmid 2-micron circle"; RL Cold Spring Harb. Symp. Quant. Biol. 49:779-787(1984). XX RN [5] RP 670-732 RX DOI; 10.1016/0092-8674(85)90339-3. RX PUBMED; 3879971. RA Andrews B.J., Proteau G.A., Beatty L.G., Sadowski P.D.; RT "The FLP recombinase of the 2 micron circle DNA of yeast: interaction with RT its target sequences"; RL Cell 40(4):795-803(1985). XX RN [6] RP 5570-5605 RX PUBMED; 2995370. RA Babineau D., Vetter D., Andrews B.J., Gronostajski R.M., Proteau G.A., RA Beatty L.G., Sadowski P.D.; RT "The FLP protein of the 2-micron plasmid of yeast. Purification of the RT protein from Escherichia coli cells expressing the cloned FLP gene"; RL J Biol Chem 260(22):12313-12319(1985). XX RN [7] RX PUBMED; 2995371. RA Gronostajski R.M., Sadowski P.D.; RT "Determination of DNA sequences essential for FLP-mediated recombination by RT a novel method"; RL J Biol Chem 260(22):12320-12327(1985). XX RN [8] RX PUBMED; 3915534. RA Sutton A., Broach J.R.; RT "Signals for transcription initiation and termination in the Saccharomyces RT cerevisiae plasmid 2 micron circle"; RL Mol. Cell. Biol. 5(10):2770-2780(1985). XX RN [9] RX PUBMED; 2427927. RA Gronostajski R.M., Sadowski P.D.; RT "The FLP recombinase of the Saccharomyces cerevisiae 2 microns plasmid RT attaches covalently to DNA via a phosphotyrosyl linkage"; RL Mol. Cell. Biol. 5(11):3274-3279(1985). XX RN [10] RP 667-739 RX DOI; 10.1073/pnas.82.21.7270. RX PUBMED; 2997780. RA Senecoff J.F., Bruckner R.C., Cox M.M.; RT "The FLP recombinase of the yeast 2-micron plasmid: characterization of its RT recombination site"; RL Proc. Natl. Acad. Sci. U.S.A. 82(21):7270-7274(1985). XX RN [11] RX PUBMED; 3540590. RA McLeod M., Craft S., Broach J.R.; RT "Identification of the crossover site during FLP-mediated recombination in RT the Saccharomyces cerevisiae plasmid 2 microns circle"; RL Mol. Cell. Biol. 6(10):3357-3367(1986). XX DR MD5; d0e43e849a68f7c4381d751af8f229bb. DR EuropePMC; PMC4008461; 24013355. DR EuropePMC; PMC5485127; 28207166. DR EuropePMC; PMC6344848; 30445476. DR StrainInfo; 492959; 0. XX CC On Feb 10, 2004 this sequence version replaced gi:4182. CC [8] sites; mRNA CAP sites and poly-adenylation sites. [9] sites; CC FLP binding. CC [7] sites; FLP cleavage. CC [11] sites; FLP-mediated recombination crossover site. Draft entry CC and clean copy sequence for [5] kindly provided by J.Senecoff, CC 24-JAN-1986. CC Yeast 2 micron plasmid contains two 599 bp inverted repeats CC separated by a large unique (UL) and a small unique (US) region. CC During recombination the UL and US regions invert producing two CC sequence forms that differ in the orientation of one unique region CC relative to the other. The A form is presented below. FLP is the CC only 2-micron circle-encoded protein needed for specific site CC recombination between the IRs of 2-micron circle. The minimal size CC of the recombination site required for efficient FLP CC recombinase-catalyzed recombination in vitro is no more than 28 bp, CC which includes parts of two 13 bp inverted repeats (positions CC 690-702 and 711-723) and all of an 8 bp spacer (703-710) [5]. The CC FLP recombinase cleaves the DNA at the boundaries of the spacer and CC becomes covalently linked to the spacer DNA [5],[9]. The CC efficiency of the recombination is reduced if the spacer in a CC recombinant site is increased or decreased by 1 bp, while the CC spacer in the second site is unaltered [5]. Recombination between CC two sites with identical 1-base pair additions or deletions is CC relatively unaffected, suggesting that pairing of sequences in the CC spacer regions is important in FLP-promoted recombination events CC [5]. The sequence asymmetry utilized by the recombinase to CC determine the orientation of the site is located uniquely within CC the spacer region. Another 13 bp direct repeat, is found at CC positions 676-688 [5]. FLP-mediated recombination involving two CC FLP sites that are inverted with respect to each other results in CC inversion of the DNA sequences between the sites [4]. If the CC participating recombination sites are in direct orientation, FLP CC promotes only the excision of the intervening DNA sequences [4]. CC The Rep 1 and Rep proteins are involved plasmid partitioning and CC protein stability. CC A start codon in phase with the Rep1 coding region is located at CC positions 1966-1964. Two CAP sites for Rep1 mRNA are located CC beyond the 'atg' codon (position 2008) at positions 2004 and 2005. CC Complete source information: CC Yeast (S.cerevisiae, strain A364A D5) DNA, clones pJDB71 [1], CC p82-6B [2], CV20 [3], pMMD2 [4], pGP20 [5], pJFS166 [10]. XX FH Key Location/Qualifiers FH FT source 1..6318 FT /organism="Saccharomyces cerevisiae" FT /plasmid="2 micron circle plasmid" FT /strain="A364A" FT /mol_type="genomic DNA" FT /clone="pJDB71, p82-6B, CV20, pMMD2, pGP20, pJFS166" FT /db_xref="taxon:4932" FT mRNA join(5549..6318,1..545) FT /product="Flp mRNA" FT CDS join(5570..6318,1..523) FT /codon_start=1 FT /product="recombinase (FLP)" FT /db_xref="GOA:P03870" FT /db_xref="InterPro:IPR005626" FT /db_xref="InterPro:IPR011010" FT /db_xref="InterPro:IPR013762" FT /db_xref="InterPro:IPR022647" FT /db_xref="PDB:1FLO" FT /db_xref="PDB:1M6X" FT /db_xref="PDB:1P4E" FT /db_xref="UniProtKB/Swiss-Prot:P03870" FT /protein_id="AAB59340.1" FT /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI FT THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI FT IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN FT SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET FT KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR FT SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR FT TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR FT YPAWNGIISQEVLDYLSSYINRRI" FT exon 1..545 FT misc_difference 157..160 FT /replace="" FT /note="conflict" FT /citation=[1] FT misc_difference 289..290 FT /replace="" FT /note="conflict" FT /citation=[1] FT repeat_region 341..939 FT /note="IR2" FT misc_difference 464..466 FT /replace="" FT /note="conflict" FT /citation=[1] FT misc_difference 558 FT /replace="" FT /note="conflict" FT /citation=[1] FT misc_difference 561 FT /replace="" FT /note="conflict" FT /citation=[1] FT misc_difference 622..624 FT /replace="" FT /note="conflict" FT /citation=[1] FT misc_difference 642 FT /replace="" FT /note="conflict" FT /citation=[1] FT misc_difference 665..666 FT /replace="" FT /note="conflict" FT /citation=[1] FT misc_binding 673..722 FT /bound_moiety="FLP recombinase" FT /note="FLP recombinase binding site A [9]" FT misc_recomb 702..703 FT misc_difference 793..794 FT /replace="" FT /note="conflict" FT /citation=[1] FT mRNA complement(836..2038) FT /note="Rep1 mRNA (alt.)" FT mRNA complement(836..2019) FT /note="Rep1 mRNA (alt.)" FT mRNA complement(836..2017) FT /note="Rep1 mRNA (alt.)" FT mRNA complement(836..2010) FT /note="Rep1 mRNA (alt.)" FT mRNA complement(836..2005) FT /note="Rep1 mRNA (alt.)" FT mRNA complement(836..2004) FT /note="Rep1 mRNA (alt.)" FT CDS complement(887..2008) FT /codon_start=1 FT /product="Rep 1 protein" FT /db_xref="GOA:P03871" FT /db_xref="InterPro:IPR008897" FT /db_xref="UniProtKB/Swiss-Prot:P03871" FT /protein_id="AAB59341.1" FT /translation="MNGERLLACIKQCIMQHFQPMVYDESRCVIETTRGTFPVPDNYKK FT YKTLAFAFVGHVLNTDDTPVIEKELDWPDPALVYNTIVDRIINHPELSQFISVAFISQL FT KATIGEGLDINVKGTLNRRGKGIRRPKGVFFRYMESPFVNTKVTAFFSYLRDYNKIASE FT YHNNTKFILTFSCQAYWASGPNFSALKNVIRCSIIHEYISKFVEREQDKGHIGDQELPP FT EEDPSRELNNVQHEVNSLTEQDAEADEGLWGEIDSLCEKWQSEAEDQTEAEIIADRIIG FT NSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHDSIKISLHYEEQHITA FT VWVYLTVKFEEHWKPVDVEVEFRCKFKERKVDG" FT mRNA 2254..2861 FT /note="D mRNA (alt.; 5' end +/- 3 bp)" FT mRNA 2254..2841 FT /note="D mRNA (alt.; 5' end +/- 3 bp)" FT CDS 2271..2816 FT /codon_start=1 FT /product="D protein" FT /db_xref="GOA:Q06891" FT /db_xref="UniProtKB/Swiss-Prot:Q06891" FT /protein_id="AAB59342.1" FT /translation="MPYKTAIDCIEELATQCFLSKLTDDDVSTFRRVCSKENDIIKLAL FT RIPRTIDYTSILRLLYDTLPLRSLSFNEALPLFCYSIDPAQQRQCDLRFYLRDVVKLAR FT PRKRLEMQKALLQWLPSLLSDVTLQLLNDIRIRFEEIQPNIRQTVLQIYDRTCYPSLNF FT EHPNLGVFPETDSIFEPV" FT repeat_region 3714..4312 FT /note="IR1" FT misc_binding 3930..3979 FT /bound_moiety="FLP recombinase" FT /note="FLP-recombinase binding site B [9]" FT misc_recomb 3941..3942 FT mRNA complement(4108..5223) FT /note="REP2 mRNA (minor alt.)" FT mRNA complement(4108..5212) FT /note="REP2 mRNA (minor alt.)" FT mRNA complement(4108..5198) FT /note="REP2 mRNA (major alt.)" FT mRNA complement(4108..5197) FT /note="REP2 mRNA (major alt.)" FT mRNA complement(4108..5196) FT /note="REP2 mRNA (major alt.)" FT mRNA complement(4108..5195) FT /note="REP2 mRNA (major alt.)" FT mRNA complement(4108..5184) FT /note="REP2 mRNA (major alt.)" FT mRNA complement(4108..5183) FT /note="REP2 mRNA (major alt.)" FT mRNA complement(4108..5182) FT /note="REP2 mRNA (major alt.)" FT CDS complement(4308..5198) FT /codon_start=1 FT /product="Rep 2 protein" FT /db_xref="GOA:P03872" FT /db_xref="UniProtKB/Swiss-Prot:P03872" FT /protein_id="AAB59343.1" FT /translation="MDDIETAKNLTVKARTAYSVWDVCRLFIEMIAPDVDIDIESKRKS FT DELLFPGYVIRPMESLTTGRPYGLDSSAEDSSVSSDSSAEVILPAAKMVKERFDSIGNG FT MLSSQEASQAAIDLMLQNNKLLDNRKQLYKSIAIIIGRLPEKDKKRATEMLMRKMDCTQ FT LLVPPAPTEEDVMKLVSVVTQLLTLVPPDRQAALIGDLFIPESLKDIFNSFNELAAENR FT LQQKKSELEGRTEVNHANTNEEVPSRRTRSRDTNARGAYKLQNTITEGPKAVPTKKRRV FT ATRVRGRKSRNTSRV" FT exon 5549..6318 FT old_sequence 5583 FT /citation=[2] XX SQ Sequence 6318 BP; 1876 A; 1284 C; 1179 G; 1979 T; 0 other; gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca 60 ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa 120 tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt 180 gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg 240 gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat 300 acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga 360 aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct 420 aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga 480 ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata 540 aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg 600 tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt 660 tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac 720 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac 780 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca 840 aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct 900 ttcgctcctt gaacttgcat ctaaactcga cctctacatc aacaggcttc caatgctctt 960 caaattttac tgtcaagtag acccatacgg ctgtaatatg ctgctcttca taatgtaagc 1020 ttatctttat cgaatcgtgt gaaaaactac taccgcgata aacctttacg gttccctgag 1080 attgaattag ttcctttagt atatgataca agacactttt gaactttgta cgacgaattt 1140 tgaggttcgc catcctctgg ctatttccaa ttatcctgtc ggctattatc tccgcctcag 1200 tttgatcttc cgcttcagac tgccattttt cacataatga atctatttca ccccacaatc 1260 cttcatccgc ctccgcatct tgttccgtta aactattgac ttcatgttgt acattgttta 1320 gttcacgaga agggtcctct tcaggcggta gctcctgatc tcctatatga cctttatcct 1380 gttctctttc cacaaactta gaaatgtatt catgaattat ggagcaccta ataacattct 1440 tcaaggcgga gaagtttggg ccagatgccc aatatgcttg acatgaaaac gtgagaatga 1500 atttagtatt attgtgatat tctgaggcaa ttttattata atctcgaaga taagagaaga 1560 atgcagtgac ctttgtattg acaaatggag attccatgta tctaaaaaat acgcctttag 1620 gccttctgat accctttccc ctgcggttta gcgtgccttt tacattaata tctaaaccct 1680 ctccgatggt ggcctttaac tgactaataa atgcaaccga tataaactgt gataattctg 1740 ggtgatttat gattcgatcg acaattgtat tgtacactag tgcaggatca ggccaatcca 1800 gttctttttc aattaccggt gtgtcgtctg tattcagtac atgtccaaca aatgcaaatg 1860 ctaacgtttt gtatttctta taattgtcag gaactggaaa agtccccctt gtcgtctcga 1920 ttacacacct actttcatcg tacaccatag gttggaagtg ctgcataata cattgcttaa 1980 tacaagcaag cagtctctcg ccattcatat ttcagttatt ttccattaca gctgatgtca 2040 ttgtatatca gcgctgtaaa aatctatctg ttacagaagg ttttcgcggt ttttataaac 2100 aaaactttcg ttacgaaatc gagcaatcac cccagctgcg tatttggaaa ttcgggaaaa 2160 agtagagcaa cgcgagttgc attttttaca ccataatgca tgattaactt cgagaaggga 2220 ttaaggctaa tttcactagt atgtttcaaa aacctcaatc tgtccattga atgccttata 2280 aaacagctat agattgcata gaagagttag ctactcaatg ctttttgtca aagcttactg 2340 atgatgatgt gtctactttc aggcgggtct gtagtaagga gaatgacatt ataaagctgg 2400 cacttagaat tccacggact atagactata ctagtatact ccgtctactg tacgatacac 2460 ttccgctcag gtccttgtcc tttaacgagg ccttaccact cttttgttac tctattgatc 2520 cagctcagca aaggcagtgt gatctaagat tctatcttcg cgatgtagta aaactagcta 2580 gaccgagaaa gagactagaa atgcaaaagg cacttctaca atggctgcca tcattattat 2640 ccgatgtgac gctgcagctt ctcaatgata ttcgaatacg ctttgaggag atacagccta 2700 atatccgaca aactgtttta cagatttacg atcgtacttg ttacccatca ttgaattttg 2760 aacatccgaa cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat 2820 atatagtcta gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg 2880 catctattgc ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc 2940 ttgcacttca atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa 3000 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac 3060 agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa 3120 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac 3180 agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt 3240 gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt 3300 ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg 3360 ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact 3420 ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg 3480 catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat 3540 agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta 3600 tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag 3660 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga 3720 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata 3780 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg 3840 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc 3900 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac 3960 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag 4020 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat 4080 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc 4140 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg 4200 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa 4260 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca taccctagaa 4320 gtattacgtg attttctgcc ccttaccctc gttgctactc tccttttttt cgtgggaacc 4380 gctttagggc cctcagtgat ggtgttttgt aatttatatg ctcctcttgc atttgtgtct 4440 ctacttcttg ttcgcctgga gggaacttct tcatttgtat tagcatggtt cacttcagtc 4500 cttccttcca actcactctt tttttgctgt aaacgattct ctgccgccag ttcattgaaa 4560 ctattgaata tatcctttag agattccggg atgaataaat cacctattaa agcagcttga 4620 cgatctggtg gaactaaagt aagcaattgg gtaacgacgc ttacgagctt cataacatct 4680 tcttccgttg gagctggtgg gactaataac tgtgtacaat ccatttttct catgagcatt 4740 tcggtagctc tcttcttgtc tttctcgggc aatcttccta ttattatagc aatagatttg 4800 tatagttgct ttctattgtc taacagcttg ttattctgta gcatcaaatc tatggcagcc 4860 tgacttgctt cttgtgaaga gagcatacca tttccaatcg aatcaaacct ttccttaacc 4920 atcttcgcag caggcaaaat tacctcagca ctggagtcag aagatacgct ggaatcttct 4980 gcgctagaat caagaccata cggcctaccg gttgtgagag attccatggg ccttatgaca 5040 tatcctggaa agagtagctc atcagactta cgtttactct ctatatcaat atctacatca 5100 ggagcaatca tttcaataaa cagccgacat acatcccaga cgctataagc tgtacgtgct 5160 tttaccgtca gattcttggc tgtttcaatg tcgtccattt tggttttctt ttaccagtat 5220 tgttcgtttg ataatgtatt cttgcttatt acattataaa atctgtgcag atcacatgtc 5280 aaaacaactt tttatcacaa gatagtaccg caaaacgaac ctgcgggccg tctaaaaatt 5340 aaggaaaagc agcaaaggtg catttttaaa atatgaaatg aagataccgc agtaccaatt 5400 attttcgcag tacaaataat gcgcggccgg tgcatttttc gaaagaacgc gagacaaaca 5460 ggacaattaa agttagtttt tcgagttagc gtgtttgaat actgcaagat acaagataaa 5520 tagagtagtt gaaactagat atcaattgca cacaagatcg gcgctaagca tgccacaatt 5580 tggtatatta tgtaaaacac cacctaaggt gcttgttcgt cagtttgtgg aaaggtttga 5640 aagaccttca ggtgagaaaa tagcattatg tgctgctgaa ctaacctatt tatgttggat 5700 gattacacat aacggaacag caatcaagag agccacattc atgagctata atactatcat 5760 aagcaattcg ctgagtttcg atattgtcaa taaatcactc cagtttaaat acaagacgca 5820 aaaagcaaca attctggaag cctcattaaa gaaattgatt cctgcttggg aatttacaat 5880 tattccttac tatggacaaa aacatcaatc tgatatcact gatattgtaa gtagtttgca 5940 attacagttc gaatcatcgg aagaagcaga taagggaaat agccacagta aaaaaatgct 6000 taaagcactt ctaagtgagg gtgaaagcat ctgggagatc actgagaaaa tactaaattc 6060 gtttgagtat acttcgagat ttacaaaaac aaaaacttta taccaattcc tcttcctagc 6120 tactttcatc aattgtggaa gattcagcga tattaagaac gttgatccga aatcatttaa 6180 attagtccaa aataagtatc tgggagtaat aatccagtgt ttagtgacag agacaaagac 6240 aagcgttagt aggcacatat acttctttag cgcaaggggt aggatcgatc cacttgtata 6300 tttggatgaa tttttgag 6318 //