ID   HQ579026; SV 1; linear; genomic DNA; STD; VRT; 652 BP.
XX
AC   HQ579026;
XX
PR   Project:PRJNA37833;
XX
DT   06-DEC-2010 (Rel. 107, Created)
DT   25-SEP-2011 (Rel. 110, Last updated, Version 2)
XX
DE   Cottus rhotheus voucher NAFF 936 cytochrome oxidase subunit 1 (COI) gene,
DE   partial cds; mitochondrial.
XX
KW   BARCODE.
XX
OS   Cottus rhotheus (torrent sculpin)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Cottales; Cottidae; Cottus.
OG   Mitochondrion
XX
RN   [1]
RP   1-652
RX   DOI; 10.1073/pnas.1016437108.
RX   PUBMED; 21670289.
RA   April J., Mayden R.L., Hanner R.H., Bernatchez L.;
RT   "Genetic calibration of species diversity among North America's freshwater
RT   fishes";
RL   Proc. Natl. Acad. Sci. U.S.A. 108(26):10602-10607(2011).
XX
RN   [2]
RP   1-652
RG   International Barcode of Life (iBOL)
RA   ;
RT   "iBOL Data Release";
RL   Unpublished.
XX
RN   [3]
RP   1-652
RG   International Barcode of Life (iBOL)
RA   ;
RT   ;
RL   Submitted (03-NOV-2010) to the INSDC.
RL   Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd West,
RL   Guelph, Ontario N1G2W1, Canada
XX
DR   MD5; e02a16e4661da7f08045aa4a37954321.
XX
CC   ##International Barcode of Life (iBOL)Data-START##
CC   Barcode Index Number :: BOLD:AAA5230
CC   Order Assignment     :: Scorpaeniformes
CC   iBOL Working Group   :: iBOL:WG1.1
CC   iBOL Release Status  :: Phase 2
CC   ##International Barcode of Life (iBOL)Data-END##
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..652
FT                   /organism="Cottus rhotheus"
FT                   /organelle="mitochondrion"
FT                   /mol_type="genomic DNA"
FT                   /country="USA:Washington, Snohomish, Armstrong Creek"
FT                   /lat_lon="48.24 N 122.14 W"
FT                   /specimen_voucher="NAFF 936"
FT                   /PCR_primers="fwd_seq:
FT                   tgtaaaacgacggccagtcaaccaaccacaaagacattggcac, rev_seq:
FT                   caggaaacagctatgacacttcagggtgaccgaagaatcagaa"
FT                   /PCR_primers="fwd_seq:
FT                   tgtaaaacgacggccagtcgactaatcataaagatatcggcac, rev_seq:
FT                   caggaaacagctatgacacctcagggtgtccgaaraaycaraa"
FT                   /db_xref="taxon:214921"
FT   gene            <1..>652
FT                   /gene="COI"
FT   CDS             <1..>652
FT                   /codon_start=2
FT                   /transl_table=2
FT                   /gene="COI"
FT                   /product="cytochrome oxidase subunit 1"
FT                   /db_xref="GOA:B2LI81"
FT                   /db_xref="InterPro:IPR000883"
FT                   /db_xref="InterPro:IPR023616"
FT                   /db_xref="InterPro:IPR036927"
FT                   /db_xref="UniProtKB/TrEMBL:B2LI81"
FT                   /protein_id="ADR14815.1"
FT                   /translation="LYLVFGAWAGMVGTALSLLIRAELSQPGALLGDDQIYNVIVTAHA
FT                   FVMIFFMVMPIMIGGFGNWLVPLMIGAPDMAFPRMNNMSFWLLPPSFLLLLASSGVEAG
FT                   AGTGWTVYPPLAGNLAHAGASVDLTIFSLHLAGISSILGAINFITTIINMKPPAISQYQ
FT                   TPLFVWSVLITAVLLLLSLPVLAAGITMLLTDRNLNTTFFDPAGGGDPILYQHL"
XX
SQ   Sequence 652 BP; 152 A; 194 C; 114 G; 192 T; 0 other;
     cctatatcta gtatttggtg cttgagccgg aatagtaggc acagctttaa gcctcctaat        60
     tcgagcagaa ctaagccaac ccggcgccct tttgggggac gaccagattt ataatgtaat       120
     tgttacagcc catgctttcg taataatttt ctttatagta ataccaatta taatcggagg       180
     tttcgggaac tgactcgttc ccctaatgat tggcgcccct gatatggcct ttcctcgaat       240
     aaataatatg agcttttgac ttcttccccc atctttttta ctcctccttg cctcttcggg       300
     agtcgaagca ggggccggaa ccgggtgaac agtctatccg cccctcgccg gaaacctcgc       360
     ccacgcagga gcctctgttg acctaacaat cttctccctt cacctagcag gtatctcctc       420
     tattcttgga gcaatcaact ttatcacaac tattattaac ataaaacccc ctgctatttc       480
     acaataccag accccgctct ttgtatgatc tgttcttatt actgccgtcc tactgcttct       540
     ttccctcccc gttcttgccg ccggtatcac aatactcctg acagaccgaa atcttaacac       600
     caccttcttt gaccctgccg gaggaggaga cccaatcctt taccaacatc tc               652
//