ID   HQ557143; SV 1; linear; genomic DNA; STD; VRT; 652 BP.
XX
AC   HQ557143;
XX
PR   Project:PRJNA37833;
XX
DT   06-DEC-2010 (Rel. 107, Created)
DT   25-SEP-2011 (Rel. 110, Last updated, Version 2)
XX
DE   Cottus girardi voucher NAFF 2989 cytochrome oxidase subunit 1 (COI) gene,
DE   partial cds; mitochondrial.
XX
KW   BARCODE.
XX
OS   Cottus girardi (Potomac sculpin)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Cottales; Cottidae; Cottus.
OG   Mitochondrion
XX
RN   [1]
RP   1-652
RX   DOI; 10.1073/pnas.1016437108.
RX   PUBMED; 21670289.
RA   April J., Mayden R.L., Hanner R.H., Bernatchez L.;
RT   "Genetic calibration of species diversity among North America's freshwater
RT   fishes";
RL   Proc. Natl. Acad. Sci. U.S.A. 108(26):10602-10607(2011).
XX
RN   [2]
RP   1-652
RG   International Barcode of Life (iBOL)
RA   ;
RT   "iBOL Data Release";
RL   Unpublished.
XX
RN   [3]
RP   1-652
RG   International Barcode of Life (iBOL)
RA   ;
RT   ;
RL   Submitted (02-NOV-2010) to the INSDC.
RL   Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd West,
RL   Guelph, Ontario N1G2W1, Canada
XX
DR   MD5; 4ba91d458bc8564412a4657c0d6b407a.
XX
CC   ##International Barcode of Life (iBOL)Data-START##
CC   Barcode Index Number :: BOLD:AAE9340
CC   Order Assignment     :: Scorpaeniformes
CC   iBOL Working Group   :: iBOL:WG1.1
CC   iBOL Release Status  :: Phase 2
CC   ##International Barcode of Life (iBOL)Data-END##
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..652
FT                   /organism="Cottus girardi"
FT                   /organelle="mitochondrion"
FT                   /mol_type="genomic DNA"
FT                   /country="USA:Maryland, Montgomery, Goshen Branch at
FT                   Brinkman Road, NW of Rockville"
FT                   /lat_lon="39.2 N 77.2 W"
FT                   /specimen_voucher="NAFF 2989"
FT                   /collected_by="DA Neely"
FT                   /collection_date="17-Jul-1994"
FT                   /PCR_primers="fwd_seq:
FT                   tgtaaaacgacggccagtcaaccaaccacaaagacattggcac, rev_seq:
FT                   caggaaacagctatgacacttcagggtgaccgaagaatcagaa"
FT                   /PCR_primers="fwd_seq:
FT                   tgtaaaacgacggccagtcgactaatcataaagatatcggcac, rev_seq:
FT                   caggaaacagctatgacacctcagggtgtccgaaraaycaraa"
FT                   /db_xref="taxon:304274"
FT   gene            <1..>652
FT                   /gene="COI"
FT   CDS             <1..>652
FT                   /codon_start=2
FT                   /transl_table=2
FT                   /gene="COI"
FT                   /product="cytochrome oxidase subunit 1"
FT                   /db_xref="GOA:E5MWA7"
FT                   /db_xref="InterPro:IPR000883"
FT                   /db_xref="InterPro:IPR023616"
FT                   /db_xref="InterPro:IPR036927"
FT                   /db_xref="UniProtKB/TrEMBL:E5MWA7"
FT                   /protein_id="ADQ50141.1"
FT                   /translation="LYLVFGAWAGMVGTALSLLIRAELSQPGALLGDDQIYNVIVTAHA
FT                   FVMIFFMVMPIMIGGFGNWLVPLMIGAPDMAFPRMNNMSFWLLPPSFLLLLASSGVEAG
FT                   AGTGWTVYPPLAGNLAHAGASVDLTIFSLHLAGISSILGAINFITTIINMKPPAISQYQ
FT                   TPLFVWSVLITAVLLLLSLPVLAAGITMLLTDRNLNTTFFDPAGGGDPILYQHL"
XX
SQ   Sequence 652 BP; 151 A; 194 C; 115 G; 192 T; 0 other;
     cctatatcta gtatttggtg cttgagccgg aatagtaggc acagctttaa gcctcctaat        60
     tcgggcagaa ctaagccaac ccggcgccct tttaggggac gaccagattt ataatgtaat       120
     tgttacggcc catgctttcg taataatttt ctttatagta ataccaatta taatcggagg       180
     tttcgggaac tgactcgttc ctctaatgat tggcgcccct gatatggcct ttcctcgaat       240
     gaataatatg agcttttgac ttcttccccc atctttttta ctcctcctcg cctcttcggg       300
     agtcgaagca ggggccggaa ccggatgaac agtctacccg cccctcgccg gaaatctcgc       360
     ccacgcagga gcctctgttg acctaacaat cttctccctt cacctagcag gtatttcctc       420
     tattcttgga gcaatcaact ttatcacaac tattattaac atgaaacccc ctgctatttc       480
     acaataccag acccctctct ttgtatgatc tgttcttatt actgccgtcc tactgcttct       540
     ttccctcccc gttcttgccg ccggcatcac aatactactg acagaccgaa atcttaacac       600
     caccttcttt gaccctgccg gaggaggaga cccaatcctt taccaacacc tc               652
//