ID   HO303361; SV 1; linear; mRNA; EST; PLN; 159 BP.
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AC   HO303361;
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DT   07-SEP-2010 (Rel. 106, Created)
DT   07-SEP-2010 (Rel. 106, Last updated, Version 1)
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DE   CFCB2631.b1 CFCB Panicum virgatum WS4U normalized leaf Panicum virgatum
DE   cDNA clone CFCB2631 5', mRNA sequence.
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KW   EST.
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OS   Panicum virgatum (switchgrass)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Panicodae; Paniceae; Panicinae; Panicum;
OC   Panicum sect. Hiantes.
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RN   [1]
RP   1-159
RA   Lucas S., Rokhsar D., Wang M., Lindquist E.A., Buckler E.S., Costich D.E.,
RA   Shi A., Casler M.D., Cherney J.H.;
RT   "DOE Joint Genome Institute/Cornell University Panicum virgatum EST
RT   project";
RL   Unpublished.
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DR   MD5; cdd619a08bc74520adbd2b8dab5ab6ab.
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CC   Contact: Lindquist,E.A.
CC   DOE Joint Genome Institute
CC   2800 Mitchell Drive, Walnut Creek, CA 94598, USA
CC   Tel: 925 296 5600
CC   Fax: 925 296 5710
CC   Email: cdna@jgi-psf.org
CC   Tissue Procurement: Laboratory of E. Buckler (Cornell University)
CC   cDNA Library Preparation: DOE Joint Genome Institute:
CC   http://www.jgi.doe.gov cDNA Library Arrayed by: DOE Joint Genome
CC   Institute: http://www.jgi.doe.gov DNA Sequencing: DOE Joint Genome
CC   Institute http://www.jgi.doe.gov Clone Distribution: NULL
CC   Plate: CFCB 0025  row: N  column: 10.
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FH   Key             Location/Qualifiers
FH
FT   source          1..159
FT                   /organism="Panicum virgatum"
FT                   /lab_host="DH10B"
FT                   /cultivar="WS4U"
FT                   /mol_type="mRNA"
FT                   /clone_lib="CFCB Panicum virgatum WS4U normalized leaf"
FT                   /clone="CFCB2631"
FT                   /tissue_type="leaf"
FT                   /note="Vector: pDNR-LIB; Site_1: SfiIA; Site_2: SfiIB; The
FT                   library was made from oligo(dT)-primed cDNA and cloned into
FT                   vector pDNR-LIB (Clontech Laboratories, Inc., Mountain
FT                   View, CA) using the SMART system (BD Biosciences, San Jose,
FT                   CA). To generate the first strand, polyA RNA was primed
FT                   with an oligo(dT) primer
FT                   (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G,
FT                   or C). The second strand was synthesized by PCR using
FT                   primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'
FT                   followed by digestion of the SfiI sites. Normalized library
FT                   was created and sequencing performed by the DOE Joint
FT                   Genome Institute (Walnut Creek, CA)."
FT                   /db_xref="taxon:38727"
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SQ   Sequence 159 BP; 41 A; 34 C; 30 G; 54 T; 0 other;
     tctttttagg gtagattttg atagaggttt gtatatctag ctgatagatg accaccacgt        60
     tttttctcat gtacatgtac cctccactgg gaaaagacga aaactggtaa ccttgttttc       120
     aaaatcaaag ggaattgccc gcctgctttt cctttaccc                              159
//