ID   G26783; SV 1; linear; genomic DNA; STS; HUM; 298 BP.
XX
AC   G26783;
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DT   06-JUN-1996 (Rel. 47, Created)
DT   27-APR-2000 (Rel. 63, Last updated, Version 3)
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DE   human STS STS_U30246, sequence tagged site.
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KW   primer; sequence tagged site; STS; STS sequence.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-298
RA   Hudson T.;
RT   "Whitehead Institute/MIT Center for Genome Research; Physically Mapped
RT   STSs";
RL   Unpublished.
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DR   MD5; c12ca65f7a402b8a2bc0532686eb6ffa.
XX
CC   Contact: Thomas Hudson
CC   Whitehead Institute/MIT Center for Genome Research
CC   Whitehead Institute for Biomedical Research
CC   9 Cambridge Center, Cambridge MA 02142 USA
CC   Tel: 617 252 1900
CC   Fax: 617 252 1902
CC   Email: thudson@genome.wi.mit.edu
CC   Primer A: GACAGCCCTCCAGAATGGTA
CC   Primer B: TCAGTTCAACAGAGAAGAATCACC
CC   STS size: 218
CC   PCR Profile:
CC   Presoak:
CC   Denaturation:
CC   Annealing: 56 degrees C
CC   Polymerization:
CC   PCR Cycles: 35
CC   Thermal Cycler:
CC   Protocol:
CC   Template: 10 ng
CC   Primer: each 5 pM
CC   dNTPs: each 4 nM
CC   Taq Polymerase: 0.025 units/ul
CC   Total Vol: 20 ul
CC   Buffer:
CC   MgCl2: 1.5 mM
CC   KCl: 50 mM
CC   Tris-HCL: 10 mM
CC   pH: 9.3
CC   Derived from dbEST (genbank accession U30246).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..298
FT                   /organism="Homo sapiens"
FT                   /map="408.5 cR from top of Chr5 linkage group"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:9606"
FT   STS             19..236
FT   primer_bind     19..38
FT   primer_bind     complement(213..236)
XX
SQ   Sequence 298 BP; 84 A; 54 C; 57 G; 103 T; 0 other;
     taaatgttct atacagtgga cagccctcca gaatggtact tcagtgccta gtgtagtaac        60
     ctgaaatctt caatgacaca ttaacatcac aatggcgaat ggtgactttt ctttcacgat       120
     ttcattaatt tgaaagcaca caggaaagct tgctccattg ataacgtgta tggagacttc       180
     ggttttagtc aattccatat ctcaatctta atggtgattc ttctctgttg aactgaagtt       240
     tgtgagagta gttttccttt gctacttgaa tagcaataaa agcgtgttaa ctttttgg         298
//