ID   G26781; SV 1; linear; genomic DNA; STS; HUM; 337 BP.
XX
AC   G26781;
XX
DT   06-JUN-1996 (Rel. 47, Created)
DT   27-APR-2000 (Rel. 63, Last updated, Version 3)
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DE   human STS STS_M29960, sequence tagged site.
XX
KW   primer; sequence tagged site; STS; STS sequence.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-337
RA   Hudson T.;
RT   "Whitehead Institute/MIT Center for Genome Research; Physically Mapped
RT   STSs";
RL   Unpublished.
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DR   MD5; 23ee303c390a158c069d49d7dd667db0.
XX
CC   Contact: Thomas Hudson
CC   Whitehead Institute/MIT Center for Genome Research
CC   Whitehead Institute for Biomedical Research
CC   9 Cambridge Center, Cambridge MA 02142 USA
CC   Tel: 617 252 1900
CC   Fax: 617 252 1902
CC   Email: thudson@genome.wi.mit.edu
CC   Primer A: AATTGAACTCACTGCTTTTGAGG
CC   Primer B: CAGCTTCAGGTATCATCTTCACC
CC   STS size: 206
CC   PCR Profile:
CC   Presoak:
CC   Denaturation:
CC   Annealing: 56 degrees C
CC   Polymerization:
CC   PCR Cycles: 35
CC   Thermal Cycler:
CC   Protocol:
CC   Template: 10 ng
CC   Primer: each 5 pM
CC   dNTPs: each 4 nM
CC   Taq Polymerase: 0.025 units/ul
CC   Total Vol: 20 ul
CC   Buffer:
CC   MgCl2: 1.5 mM
CC   KCl: 50 mM
CC   Tris-HCL: 10 mM
CC   pH: 9.3
CC   Derived from dbEST (genbank accession M29960).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..337
FT                   /organism="Homo sapiens"
FT                   /map="464.3 cR from top of Chr12 linkage group"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:9606"
FT   STS             61..266
FT   primer_bind     61..83
FT   primer_bind     complement(244..266)
XX
SQ   Sequence 337 BP; 109 A; 59 C; 51 G; 118 T; 0 other;
     tgaaaactgt gactgcagtg ctgtaaactt aactgttctt tgccagaaca caagacacca        60
     aattgaactc actgcttttg aggcatctgg aaatttttac tttaaaaagt aaccagaatc       120
     caaggtattt ttattttagc ttcccttaag aatttttgaa gtgactgggc aggcagcaga       180
     aattaaatga atttttcttc ctgattcctt taaatgaata tgaaacacta caaatttatt       240
     cttggtgaag atgatacctg aagctgtcac ctcttgatta tctaaactaa gcgctcattc       300
     tattttataa aacaaataaa ttagtctctt ttttctg                                337
//