ID   G26779; SV 1; linear; genomic DNA; STS; HUM; 570 BP.
XX
AC   G26779;
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DT   06-JUN-1996 (Rel. 47, Created)
DT   27-APR-2000 (Rel. 63, Last updated, Version 3)
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DE   human STS STS_U17899, sequence tagged site.
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KW   primer; sequence tagged site; STS; STS sequence.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-570
RA   Hudson T.;
RT   "Whitehead Institute/MIT Center for Genome Research; Physically Mapped
RT   STSs";
RL   Unpublished.
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DR   MD5; d022c4789b4d83cd64435851978bd6b8.
XX
CC   Contact: Thomas Hudson
CC   Whitehead Institute/MIT Center for Genome Research
CC   Whitehead Institute for Biomedical Research
CC   9 Cambridge Center, Cambridge MA 02142 USA
CC   Tel: 617 252 1900
CC   Fax: 617 252 1902
CC   Email: thudson@genome.wi.mit.edu
CC   Primer A: GTGCCTCTTCCACTCTGGAG
CC   Primer B: GTACCCAGACACAGAAAGTTTTAGG
CC   STS size: 216
CC   PCR Profile:
CC   Presoak:
CC   Denaturation:
CC   Annealing: 56 degrees C
CC   Polymerization:
CC   PCR Cycles: 35
CC   Thermal Cycler:
CC   Protocol:
CC   Template: 10 ng
CC   Primer: each 5 pM
CC   dNTPs: each 4 nM
CC   Taq Polymerase: 0.025 units/ul
CC   Total Vol: 20 ul
CC   Buffer:
CC   MgCl2: 1.5 mM
CC   KCl: 50 mM
CC   Tris-HCL: 10 mM
CC   pH: 9.3
CC   Derived from dbEST (genbank accession U17899).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..570
FT                   /organism="Homo sapiens"
FT                   /map="389.8 cR from top of Chr11 linkage group"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:9606"
FT   STS             56..271
FT   primer_bind     56..75
FT   primer_bind     complement(247..271)
XX
SQ   Sequence 570 BP; 161 A; 103 C; 114 G; 192 T; 0 other;
     tgaaaatgat ttatgcaagt ttaagattct gctcctaagg gtaggagaga acttggtgcc        60
     tcttccactc tggagtgaag ttaatgaaag tctttttcct tttccaaaac ccaacctgaa       120
     ccagttcttt cttgagacag actatactga gacaacaagt tgtcaccagc agaagataga       180
     taatatgacc tttattaact tgatgaatta acttaaccaa gagggtattt gtagtttact       240
     atttacccta aaactttctg tgtctgggta ccctctgagt aggcctataa ttcctacctt       300
     gactgtgtgc atcatttgta agctagcaga tctatgtggt gaaaatgcac aggagcttgg       360
     tagactgcgg gggaaagaga gagctccttt cgccatgttt taccagtctg ctgttataac       420
     ctcttaggtt gtatccttta atttccagcc ttttaggtta gtttctgtaa cagaacaagt       480
     gagtctggga tgaagtcctc aaagtacttc aaatggtaat tgttttgttt ttgtaatagc       540
     ttaacaaata aacctaggtt ttctatatta                                        570
//