ID   G26778; SV 1; linear; genomic DNA; STS; HUM; 1504 BP.
XX
AC   G26778;
XX
DT   06-JUN-1996 (Rel. 47, Created)
DT   27-APR-2000 (Rel. 63, Last updated, Version 3)
XX
DE   human STS STS_X68830, sequence tagged site.
XX
KW   primer; sequence tagged site; STS; STS sequence.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-1504
RA   Hudson T.;
RT   "Whitehead Institute/MIT Center for Genome Research; Physically Mapped
RT   STSs";
RL   Unpublished.
XX
DR   MD5; 44aebdd0ad3b468f209d1a69fcfdc25d.
XX
CC   Contact: Thomas Hudson
CC   Whitehead Institute/MIT Center for Genome Research
CC   Whitehead Institute for Biomedical Research
CC   9 Cambridge Center, Cambridge MA 02142 USA
CC   Tel: 617 252 1900
CC   Fax: 617 252 1902
CC   Email: thudson@genome.wi.mit.edu
CC   Primer A: TAACAGTGCCCTTTTCATCTCC
CC   Primer B: CTGTGCCACTGAGATATAGGTCC
CC   STS size: 217
CC   PCR Profile:
CC   Presoak:
CC   Denaturation:
CC   Annealing: 56 degrees C
CC   Polymerization:
CC   PCR Cycles: 35
CC   Thermal Cycler:
CC   Protocol:
CC   Template: 10 ng
CC   Primer: each 5 pM
CC   dNTPs: each 4 nM
CC   Taq Polymerase: 0.025 units/ul
CC   Total Vol: 20 ul
CC   Buffer:
CC   MgCl2: 1.5 mM
CC   KCl: 50 mM
CC   Tris-HCL: 10 mM
CC   pH: 9.3
CC   Derived from dbEST (genbank accession X68830).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1504
FT                   /organism="Homo sapiens"
FT                   /map="158.9 cR from top of Chr12 linkage group"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:9606"
FT   STS             60..276
FT   primer_bind     60..81
FT   primer_bind     complement(254..276)
XX
SQ   Sequence 1504 BP; 396 A; 178 C; 239 G; 446 T; 245 other;
     tagaggacaa tgtaactcta tagttattgt tttatgttct agtgatttcc tgtataattt        60
     aacagtgccc ttttcatctc cagtgtgaat atatggtctg tgtgtctgat gtttgttgct       120
     aggacatata ccttctcaaa agattgtttt atatgtagta ctaactaagg tcccataata       180
     aaaagatagt atcttttaaa atgaaatgtt tttgctatag atttgtattt taaaacataa       240
     gaacgtcatt ttgggaccta tatctcagtg gcacaggttt aagaacgaag gagaaaaagg       300
     tagtttgaac cttggtaaat tgtaaacagc taataatgaa gttattcttg acatgagaaa       360
     atcagtaatt ggaccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       420
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       480
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       540
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       600
     nnnnnnnnnn nnnnnnnnnn nagcctgggt ggcagagtga gactcgtctc aaaaaaaaga       660
     aagaaaatta gtaattgtaa gtacccctga taagcaaatt agtaattgtc aatacccctg       720
     ttaagcaatt cctttttgca gtatatttct gaaatgacag aatgctgttt taaaaacaaa       780
     gaaataaaat cctgctcctg actcggtcaa aatatttttt aaagtctatt gtttgttgtg       840
     cttgctggta ctaagaggct atttaaaagt ataaaactgc tttgtatcca tgagggtttc       900
     attgtgtgtt agcagcagtg agcttctatt aaatgtatat gtcatttatt ttgtttaagt       960
     ggctttcagc aaacctcagt catattctta tgcagggtat tgcgaaacaa cttgtgttct      1020
     attaatcgtg tcttcaatta aaagaccaca gacttctgga aactctttgc tgtataagaa      1080
     ttatttcttt tgtttaacaa attagacatt tctggcagag gttatgtata tgatacactt      1140
     tttttgatag cagctgcaat gttggacaga agatgaaatg ctttgctttg agtcagattc      1200
     ttatgaatat ctgcttttcc ctgactttga gttaggtagc tttggaagta gcattaattc      1260
     agataaactg ccatcatgct gcgttatgcc atttctaaag accactcaac ttgtactttt      1320
     aaaaaaatag aaaaaatagc atttcaatct aagtggaaat ttgactcatt gacttacatt      1380
     tctaagttaa aatttccctt tatgaagtgt gccttaggtt accaaattgt agaggctttc      1440
     gttggtggtg gtaattggta gcggtagtga gtgtatagag gcagggaaat atatttataa      1500
     taaa                                                                   1504
//