ID   G26776; SV 1; linear; genomic DNA; STS; HUM; 1364 BP.
XX
AC   G26776;
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DT   06-JUN-1996 (Rel. 47, Created)
DT   27-APR-2000 (Rel. 63, Last updated, Version 3)
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DE   human STS STS_L40393, sequence tagged site.
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KW   primer; sequence tagged site; STS; STS sequence.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-1364
RA   Hudson T.;
RT   "Whitehead Institute/MIT Center for Genome Research; Physically Mapped
RT   STSs";
RL   Unpublished.
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DR   MD5; 818b08c35dd655e00fb93b29215c19bf.
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CC   Contact: Thomas Hudson
CC   Whitehead Institute/MIT Center for Genome Research
CC   Whitehead Institute for Biomedical Research
CC   9 Cambridge Center, Cambridge MA 02142 USA
CC   Tel: 617 252 1900
CC   Fax: 617 252 1902
CC   Email: thudson@genome.wi.mit.edu
CC   Primer A: TCTTGTCCATACCAGACAGGG
CC   Primer B: GACTTCTTTAGCCTAATTGCAAGG
CC   STS size: 202
CC   PCR Profile:
CC   Presoak:
CC   Denaturation:
CC   Annealing: 56 degrees C
CC   Polymerization:
CC   PCR Cycles: 35
CC   Thermal Cycler:
CC   Protocol:
CC   Template: 10 ng
CC   Primer: each 5 pM
CC   dNTPs: each 4 nM
CC   Taq Polymerase: 0.025 units/ul
CC   Total Vol: 20 ul
CC   Buffer:
CC   MgCl2: 1.5 mM
CC   KCl: 50 mM
CC   Tris-HCL: 10 mM
CC   pH: 9.3
CC   Derived from dbEST (genbank accession L40393).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1364
FT                   /organism="Homo sapiens"
FT                   /map="258.8 cR from top of Chr14 linkage group"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:9606"
FT   STS             24..225
FT   primer_bind     24..44
FT   primer_bind     complement(202..225)
XX
SQ   Sequence 1364 BP; 418 A; 240 C; 277 G; 426 T; 3 other;
     taagcaatca ttatggctat gtatcttgtc cataccagac agggagcagg gggtagcggt        60
     caaaggagcn aaacagantt tgtctcctga ttagtactct tttcactaat cccaaaggtc       120
     ccaaggaaca agtccaggcc cagagtactg tgaggggtga ttttgaaaga catgggaaaa       180
     agcattccta gagaaaagct gccttgcaat taggctaaag aagtcaagga aatgttgctt       240
     tctgtactcc ctcttccctt acccccttac aaatctctgg caacagagag gcaaagtatc       300
     tgaacaagaa tctatattcc aagcacattt actgaaatgt aaaacacaac aggaagcaaa       360
     gcaatgtccc tttgtttttc aggccattca cctgcctcct gtcagtagtg gcctgtatta       420
     gagatcaaga agagtggttt gtgctcaggc tgggaacaga gaggcacgct atgctgccag       480
     aattcccagg agggcatatc agcaactgcc cagcagagct atattttggg ggagaagttg       540
     agcttccatt ttgagtaaca gaataaatat tatatatatc aaaagccaaa atctttattt       600
     ttatgcattt agaatatttt aaatagttct cagatattaa gaagttgtat gagttgtaag       660
     taatcttgcc aaaggtaaag gggctagttg taagaaattg tacatnagat tgatttatca       720
     ttgatgccta ctgaaataaa aagaggaaag gctggaagca tgcagacagg atccctagct       780
     tgttttctgt cagtcattca ttgtaagtag cacattgcaa caacaatcat gcttatgacc       840
     aatacagtca ctaggttgta gtttttttta aataaaggaa aagcagtatt gtcctggttt       900
     taaacctatg atggaattct aatgtcatta ttttaatgga atcaatcgaa atatgctcta       960
     tagagaatat atcttttata tattgctgca gtttccttat gttaatcctt taacactaag      1020
     gtaacatgac ataatcatac catagaaggg aacacaggtt accatattgg tttgtaatat      1080
     gggtcttggt gggttttgtt ttatccttta aattttgttc ccatgagttt tgtggggatg      1140
     gggattctgg ttttattagc tttgtgtgtg tcctcttccc ccaaaccccc ttttggtgag      1200
     aacatcccct tgacagttgc agcctcttga cctcggataa caataagaga gctcatctca      1260
     tttttacttt tgaacgttgg cgcttacaat caaatgtaag ttatatatat ttgtactgat      1320
     gaaaatttat aatctgcttt aacaaaaata aatgttcatg gtag                       1364
//