ID BX571858; SV 1; circular; genomic DNA; STD; PRO; 20652 BP. XX AC BX571858; XX PR Project:PRJNA266; XX DT 23-JUN-2004 (Rel. 80, Created) DT 29-JAN-2016 (Rel. 127, Last updated, Version 6) XX DE Staphylococcus aureus plasmid pSAS, complete genome. XX KW . XX OS Staphylococcus aureus subsp. aureus MSSA476 OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; OC Staphylococcus. OG Plasmid pSAS XX RN [1] RP 1-20652 RX DOI; 10.1073/pnas.0402521101. RX PUBMED; 15213324. RA Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., RA Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., RA Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., RA Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., RA Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., RA Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., RA Rabbinowitsch E., Rutherford K., Sanders M., Sharp S., Simmonds M., RA Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.; RT "Complete genomes of two clinical Staphylococcus aureus strains: evidence RT for the rapid evolution of virulence and drug resistance"; RL Proc. Natl. Acad. Sci. U.S.A. 101(26):9786-9791(2004). XX RN [2] RP 1-20652 RA Holden M.T.G.; RT ; RL Submitted (23-JUN-2004) to the INSDC. RL Submitted on behalf of the Pathogen Sequencing Unit, Sanger Institute, RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL mh3@sanger.ac.uk XX DR MD5; 0b9abe53c1077769fa7f12ea28e0b66d. DR BioSample; SAMEA1705922. DR EuropePMC; PMC3406130; 22685157. DR EuropePMC; PMC3530869; 23275717. DR EuropePMC; PMC470752; 15213324. DR RFAM; RF01797; fstAT. DR StrainInfo; 844898; 0. XX FH Key Location/Qualifiers FH FT source 1..20652 FT /organism="Staphylococcus aureus subsp. aureus MSSA476" FT /plasmid="pSAS" FT /sub_species="aureus" FT /strain="MSSA476" FT /mol_type="genomic DNA" FT /db_xref="taxon:282459" FT CDS 12..638 FT /transl_table=11 FT /locus_tag="pSAS01" FT /product="putative ABC transporter ATP binding protein" FT /note="Similar to Lactococcus lactis hypothetical ATP FT binding protein TR:Q9L650 (EMBL:AF242367) (207 aa) fasta FT scores: E(): 9.5e-26, 49.75% id in 207 aa, and to FT Streptococcus pneumoniae hypothetical protein TR:Q9ZHB1 FT (EMBL:AF068902) (213 aa) fasta scores: E(): 3.2e-24, 45.23% FT id in 210 aa" FT /protein_id="CAG43225.1" FT /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN FT IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN FT KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD FT TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS" FT misc_feature 90..629 FT /note="Pfam match to entry PF00005 ABC_tran, ABC FT transporter, score 191.1, E-value 1.1e-54" FT misc_feature 111..134 FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)." FT misc_feature 405..449 FT /note="PS00211 ABC transporters family signature." FT CDS join(876..1178,1182..1502,1506..2144) FT /pseudo FT /transl_table=11 FT /locus_tag="pSAS02" FT /product="putative plasmid recombination enzyme FT (pseudogene)" FT /note="Similar to Staphylococcus aureus plasmid pT181 FT recombination enzyme SW:PRE3_STAAU (P03864) (413 aa) fasta FT scores: E(): 1.3e-64, 55.92% id in 422 aa, and to FT Staphylococcus cohnii recombination/mobilization protein FT Pre TR:O87277 (EMBL:AF015628) (420 aa) fasta scores: E(): FT 9.9e-28, 31.64% id in 433 aa. Contains nonsense mutations FT (amber) after codons 101 and 209" FT /db_xref="PSEUDO:CAG43228.1" FT misc_feature 876..1466 FT /note="Pfam match to entry PF01076 Mob_Pre, Plasmid FT recombination enzyme, score 326.3, E-value 2.1e-95" FT CDS complement(2179..2487) FT /transl_table=11 FT /locus_tag="pSAS03" FT /product="hypothetical protein" FT /note="No significant database matches" FT /protein_id="CAG43231.1" FT /translation="MNIHSHLKTFIHNKLLYYQCQTIKGVQLFKTLLLCGKNTPFMWQK FT HSFYVAKTLLLCGKNTPLGIEIPCCSRAEQAPKKVFKKIYKNILKSSRGLPIFHPKK" FT CDS 2527..3387 FT /transl_table=11 FT /locus_tag="pSAS04" FT /product="replication protein" FT /note="Similar to Neisseria gonorrhoeae replication protein FT SW:REPA_NEIGO (P17492) (328 aa) fasta scores: E(): 6.7e-08, FT 22.09% id in 258 aa. Identical to Staphylococcus aureus FT plasmid pN315 replication protein Rep TR:Q9AC64 FT (EMBL:AP003139) (286 aa) fasta scores: E(): 6.4e-100, 100% FT id in 286 aa" FT /protein_id="CAG43232.1" FT /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL FT SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR FT EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI FT DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA FT EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL FT ELNWE" FT CDS 3474..3815 FT /transl_table=11 FT /locus_tag="pSAS05" FT /product="putative regulatory protein" FT /note="Similar to Lactococcus lactis transcriptional FT regulator LL0007 TR:Q9CJI6 (EMBL:AE006240) (107 aa) fasta FT scores: E(): 5e-09, 36.6% id in 112 aa. N-terminal region FT is highly similar to Staphylococcus aureus plasmid pN315 FT hypothetical protein SAP028 TR:Q9AC63 (EMBL:AP003139) (79 FT aa) fasta scores: E(): 1.4e-25, 98.73% id in 79 aa" FT /protein_id="CAG43234.1" FT /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD FT ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL FT VWAIHSFMG" FT misc_feature 3492..3656 FT /note="Pfam match to entry PF01381 HTH_3, Helix-turn-helix, FT score 70.5, E-value 2.1e-18" FT CDS 3834..4073 FT /transl_table=11 FT /locus_tag="pSAS06" FT /product="putative membrane protein" FT /note="No significant database matches" FT /protein_id="CAG43236.1" FT /translation="MHIRQYEKGKYFYDTLKCIDKNRRVYKRQYEIYTKSNKNFVCKRT FT KPRTIRRSSGFFGELALIVYIIFYHVYGANNQIT" FT misc_feature 3990..4049 FT /note="1 probable transmembrane helix predicted for pSAS.06 FT by TMHMM2.0 at aa 53-72" FT CDS 4274..4459 FT /transl_table=11 FT /locus_tag="pSAS07" FT /product="hypothetical protein" FT /note="Poor database matches. Similar to Staphylococcus FT aureus plasmid pN315 hypothetical protein SAP029 TR:Q9AC61 FT (EMBL:AP003139) (61 aa) fasta scores: E(): 1.1e-25, 98.36% FT id in 61 aa" FT /protein_id="CAG43238.1" FT /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII FT RVKDGLLGWRKEIVTE" FT CDS complement(4678..5421) FT /transl_table=11 FT /locus_tag="pSAS08" FT /product="replication-associated protein" FT /note="Similar to Staphylococcus aureus plasmid pSK1 FT replication-associated protein Orf245 TR:Q9L8I7 FT (EMBL:AF203376) (245 aa) fasta scores: E(): 4e-18, 45.59% FT id in 261 aa, and to Staphylococcus haemolyticus plasmid FT pNVH97A hypothetical protein TR:Q9K4M8 (EMBL:AJ400722) (245 FT aa) fasta scores: E(): 1.5e-40, 63.56% id in 247 aa" FT /protein_id="CAG43240.1" FT /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE FT KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE FT SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR FT QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND FT NDSDIEEKSEETEAKKGFWSRLFGN" FT CDS join(5832..6104,6103..6333) FT /pseudo FT /transl_table=11 FT /locus_tag="pSAS09" FT /product="putative replication initiation protein FT (fragement)" FT /note="Similar to the N-terminal regions of Staphylococcus FT aureus plasmid pSV41 putative replication initiation FT protein Rep TR:O87367 (EMBL:AF051917) (319 aa) fasta FT scores: E(): 5.6e-27, 56.29% id in 151 aa, and Enterococcus FT faecalis plasmid pCF10 regulatory protein PrgW TR:Q51642 FT (EMBL:L14285) (333 aa) fasta scores: E(): 1.7e-18, 44.64% FT id in 168 aa" FT /db_xref="PSEUDO:CAG43243.1" FT CDS join(6330..6383,6383..6505,6504..6578,6578..6655, FT 6659..6793) FT /pseudo FT /transl_table=11 FT /locus_tag="pSAS10" FT /product="putative replication protein (fragment)" FT /note="Similar to the C-terminal regions Staphylococcus FT aureus plasmid pC55s putative replication protein Rep FT TR:Q938S2 (EMBL:AY048756) (331 aa) fasta scores: E(): FT 1.8e-20, 63.63% id in 132 aa, and to Staphylococcus FT epidermidis Orf334 orf334 TR:Q53999 (EMBL:U15783) (334 aa) FT fasta scores: E(): 2e-20, 62.04% id in 137 aa" FT /db_xref="PSEUDO:CAG43244.1" FT misc_feature 6396..6791 FT /note="Pfam match to entry PF01446 Rep, Replication FT protein, score -29.1, E-value 1e-08" FT CDS 7998..8627 FT /transl_table=11 FT /gene="cadD" FT /locus_tag="pSAS11" FT /product="cadmium resistance transporter" FT /note="Almost identical to Staphylococcus aureus plasmid FT pRW001 putative cadmium resistance protein CadD TR:O05469 FT (EMBL:AF134905) (209 aa) fasta scores: E(): 2.9e-70, 99.04% FT id in 209 aa. Similar to Neisseria meningitidis cadmium FT resistance protein NMB1955 TR:Q9JXN6 (EMBL:AE002544) (208 FT aa) fasta scores: E(): 3.9e-41, 56.93% id in 209 aa" FT /protein_id="CAG43247.1" FT /translation="MRCIMIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRNIYV FT GQYLGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKEL FT NEKGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQ FT KLANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF" FT misc_feature order(8025..8093,8130..8198,8211..8270,8331..8399, FT 8409..8477,8538..8606) FT /note="6 probable transmembrane helices predicted for FT pSAS.11 by TMHMM2.0 at aa 10-32, 45-67, 72-91, 112-134, FT 138-160 and 181-203" FT misc_feature 8043..8618 FT /note="Pfam match to entry PF03596 Cad, Cadmium resistance FT transporter, score 434.5, E-value 5.8e-128" FT CDS 8646..8993 FT /transl_table=11 FT /gene="cadC" FT /locus_tag="pSAS12" FT /product="putative cadmium efflux system regulatory FT protein" FT /note="Similar to Staphylococcus aureus plasmid pI258 FT cadmium efflux system accessory protein CadC SW:CADC_STAAU FT (P20047) (122 aa) fasta scores: E(): 7.8e-13, 42.45% id in FT 106 aa, and to Staphylococcus aureus plasmids pN315 and FT pC55s, putative regulator of cadB, CadX TR:Q9AC84 FT (EMBL:AP003139) (115 aa) fasta scores: E(): 1.1e-41, 99.13% FT id in 115 aa" FT /protein_id="CAG43249.1" FT /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL FT KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI FT REFFSKNQEGF" FT misc_feature 8751..8987 FT /note="Pfam match to entry PF01022 HTH_5, Bacterial FT regulatory protein, arsR family, score 95.0, E-value 9e-26" FT misc_feature 8817..8873 FT /note="PS00846 Bacterial regulatory proteins, arsR family FT signature." FT CDS 9466..10440 FT /transl_table=11 FT /locus_tag="pSAS13" FT /product="hypothetical protein" FT /note="No significant database matches" FT /protein_id="CAG43251.1" FT /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS FT SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN FT KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLIQSVPSEIKENSGWI FT RVQDCGKNVKKIKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN FT YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL FT LQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE" FT misc_feature 10282..10347 FT /note="PS00392 DDC / GAD / HDC / TyrDC pyridoxal-phosphate FT attachment site." FT CDS complement(10560..11375) FT /transl_table=11 FT /locus_tag="pSAS14" FT /product="potential ATP-binding protein" FT /note="Similar to Staphylococcus aureus Tn552 transposable FT element potential ATP-binding protein SW:ATBP_STAAU FT (P18179) (271 aa) fasta scores: E(): 7.4e-99, 95.94% id in FT 271 aa, and to Listeria innocua plasmid pLI100 hypothetical FT protein Pli0057 TR:Q926K3 (EMBL:AL592102) (285 aa) fasta FT scores: E(): 2.8e-33, 51.97% id in 279 aa" FT /protein_id="CAG43253.1" FT /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR FT YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL FT GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG FT MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKCQDLGFDLKLEDFTDYEAIT FT TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR" FT misc_feature complement(11253..11276) FT /note="PS00017 ATP/GTP-binding site motif A (P-loop)." FT CDS complement(join(11368..12192,12191..12811)) FT /pseudo FT /transl_table=11 FT /locus_tag="pSAS15" FT /product="putative transposase (pseudogene)" FT /note="Similar to Staphylococcus aureus transposon Tn552 FT transposase SW:TRA3_STAAU (P18416) (480 aa) fasta scores: FT E(): 3.1e-176, 91.06% id in 481 aa, and to Listeria innocua FT plasmid pLI100 hypothetical protein Pli0058 TR:Q926K2 FT (EMBL:AL592102) (478 aa) fasta scores: E(): 3.5e-93, 49.58% FT id in 484 aa. Contains a frameshift after codon 207. FT Frameshift occurs at a poly C hexamer" FT /db_xref="PSEUDO:CAG43255.1" FT misc_feature complement(11795..12349) FT /note="Pfam match to entry PF00665 rve, Integrase core FT domain, score 106.6, E-value 3e-29" FT CDS complement(12783..13376) FT /transl_table=11 FT /gene="tnpR" FT /gene_synonym="binL" FT /locus_tag="pSAS16" FT /product="transposon resolvase" FT /note="Similar to Staphylococcus aureus transposon Tn552 FT resolvase TnpR SW:BINL_STAAU (P18358) (197 aa) fasta FT scores: E(): 9.2e-71, 95.93% id in 197 aa, and to FT Staphylococcus epidermidis resolvase/integrase BinR FT TR:Q93SQ9 (EMBL:AY028779) (192 aa) fasta scores: E(): FT 6.9e-66, 90.62% id in 192 aa" FT /protein_id="CAG43258.1" FT /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL FT DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ FT LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI FT KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN" FT misc_feature complement(12825..12959) FT /note="Pfam match to entry PF02796 HTH_7, Helix-turn-helix FT domain of resolvase, score 72.4, E-value 5.9e-19" FT misc_feature complement(12963..13373) FT /note="Pfam match to entry PF00239 resolvase, Resolvase, N FT terminal domain, score 284.6, E-value 7.8e-83" FT misc_feature complement(13176..13214) FT /note="PS00398 Site-specific recombinases signature 2." FT misc_feature complement(13338..13364) FT /note="PS00397 Site-specific recombinases active site." FT CDS complement(13640..14020) FT /transl_table=11 FT /gene="blaI" FT /gene_synonym="penI" FT /locus_tag="pSAS17" FT /product="penicillinase repressor" FT /note="Highly similar to Staphylococcus aureus plasmid FT pI258 penicillinase repressor BlaI SW:BLAI_STAAU (P18415) FT (126 aa) fasta scores: E(): 3.4e-39, 99.2% id in 126 aa. FT Identical to Staphylococcus haemolyticus plasmids pNVH97A FT and pNVH96 beta-lactamase repressor BlaI TR:Q9K4N1 FT (EMBL:AJ400722) (126 aa) fasta scores: E(): 1e-39, 100% id FT in 126 aa" FT /protein_id="CAG43260.1" FT /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKTI FT RTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNFAK FT NEELNNKEIEELRDILNDISKK" FT CDS complement(14010..15767) FT /transl_table=11 FT /gene="blaR1" FT /locus_tag="pSAS18" FT /product="putative beta-lactamase regulatory protein" FT /note="Similar to Staphylococcus aureus Tn552 transposable FT element regulatory protein BlaR1 SW:BLAR_STAAU (P18357) FT (585 aa) fasta scores: E(): 7.1e-205, 94.01% id in 585 aa, FT and to Staphylococcus haemolyticus plasmid pNVH96 FT beta-lactamase inducer protein BlaR1 TR:Q9F2I0 FT (EMBL:AJ302698) (585 aa) fasta scores: E(): 7.9e-203, FT 92.99% id in 585 aa" FT /protein_id="CAG43262.1" FT /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTVLA FT GLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNIITTKPVQEFATDIHKFNWDSIDN FT ICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRK FT AEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSI FT IMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTSFFNKNILS FT HSFNGKKSLLKNRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSLTDYNYK FT KPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLALFG FT LDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQL FT KQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKK FT NEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKIL FT KGMGVLNGQ" FT misc_feature complement(14019..14777) FT /note="Pfam match to entry PF00144 beta-lactamase, FT Beta-lactamase, score 132.5, E-value 4.8e-37" FT misc_feature complement(join(14775..14840,15069..15134,15207..15272, FT 15387..15452,15588..15653,15690..15755)) FT /note="6 probable transmembrane helices predicted for FT pSAS.18c by TMHMM2.0 at aa 4-26, 38-60, 105-127, 165-187, FT 211-233 and 309-331" FT CDS 15874..16719 FT /transl_table=11 FT /gene="blaZ" FT /locus_tag="pSAS19" FT /product="beta-lactamase precursor" FT /EC_number="3.5.2.6" FT /note="Similar to Staphylococcus aureus beta-lactamase FT precursor BlaZ SW:BLAC_STAAU (P00807) (281 aa) fasta FT scores: E(): 8.6e-94, 97.5% id in 281 aa, and to FT Enterococcus faecalis beta-lactamase BlaZ TR:AAA24777 FT (EMBL:M60253) (281 aa) fasta scores: E(): 8.6e-94, 97.5% id FT in 281 aa" FT /protein_id="CAG43271.1" FT /translation="MKKLIFLIVMALVLSACNSNSSHAKELNDLEKKYNAHIGVYALDT FT KSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYV FT GKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYY FT SPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPKDYKV FT ADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKEF FT " FT misc_feature 15874..15945 FT /note="Signal peptide predicted for pSAS.19 by SignalP 2.0 FT HMM (Signal peptide probabilty 1.000) with cleavage site FT probability 0.999 between residues 24 and 25" FT misc_feature 15943..16716 FT /note="Pfam match to entry PF00144 beta-lactamase, FT Beta-lactamase, score 376.1, E-value 2.2e-110" FT misc_feature 16048..16095 FT /note="PS00146 Beta-lactamase class-A active site." FT CDS 17088..17621 FT /pseudo FT /transl_table=11 FT /gene="bin" FT /gene_synonym="sin" FT /locus_tag="pSAS20" FT /product="potential DNA-invertase (fragment)" FT /note="Similar to Staphylococcus aureus Tn552 transposable FT element potential DNA-invertase Bin3 SW:BIN3_STAAU (P20384) FT (202 aa) fasta scores: E(): 6.3e-33, 80.09% id in 201 aa, FT and to Staphylococcus epidermidis plasmid pST6 recombinase FT Sin TR:Q93SQ8 (EMBL:AY028779) (202 aa) fasta scores: E(): FT 1.5e-32, 78.71% id in 202 aa. CDS contains an internal FT deletion relative to orthologues" FT /db_xref="PSEUDO:CAG43273.1" FT misc_feature 17088..17447 FT /note="Pfam match to entry PF00239 resolvase, Resolvase, N FT terminal domain, score 165.5, E-value 5.5e-47" FT misc_feature 17100..17126 FT /note="PS00397 Site-specific recombinases active site." FT misc_feature 17451..17609 FT /note="Pfam match to entry PF02796 HTH_7, Helix-turn-helix FT domain of resolvase, score 86.7, E-value 2.9e-23" FT CDS 17861..18010 FT /transl_table=11 FT /locus_tag="pSAS21" FT /product="putative membrane protein" FT /note="No significant database matches. Similar to Borrelia FT burgdorferi conserved hypothetical protein BBT02 TR:Q9S019 FT (EMBL:AE001583) (117 aa) fasta scores: E(): 1.7, 40.9% id FT in 44 aa" FT /protein_id="CAG43276.1" FT /translation="MSYRCLMIVNLRIIELILYIDEQNKQQSRKNTFRLFLLILYFIYF FT CYNK" FT misc_feature 17951..18004 FT /note="1 probable transmembrane helix predicted for pSAS.21 FT by TMHMM2.0 at aa 31-48" FT CDS 18035..18328 FT /transl_table=11 FT /locus_tag="pSAS22" FT /product="putative bacteriocin precursor" FT /note="Similar to Lactococcus lactis lactococcin 972 FT precursor LclA TR:O86283 (EMBL:AJ002203) (91 aa) fasta FT scores: E(): 0.021, 30.76% id in 78 aa. Identical to FT Staphylococcus aureus hypothetical protein SAP019 TR:Q9AC72 FT (EMBL:AP003139) (97 aa) fasta scores: E(): 4.5e-39, 100% id FT in 97 aa" FT /protein_id="CAG43278.1" FT /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH FT YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH" FT misc_feature 18035..18118 FT /note="Signal peptide predicted for pSAS.22 by SignalP 2.0 FT HMM (Signal peptide probabilty 1.000) with cleavage site FT probability 0.966 between residues 28 and 29" FT CDS 18403..20337 FT /transl_table=11 FT /locus_tag="pSAS23" FT /product="hypothetical bacteriocin immunity protein" FT /note="Similar to Lactococcus lactis hypothetical FT lactococcin 972 immunity protein LclB TR:Q9L651 FT (EMBL:AF242367) (648 aa) fasta scores: E(): 1.7e-13, 21.9% FT id in 662 aa. Almost identical to Staphylococcus aureus FT hypothetical protein SAP020 TR:Q9AC71 (EMBL:AP003139) (644 FT aa) fasta scores: E(): 0, 99.53% id in 644 aa" FT /protein_id="CAG43280.1" FT /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD FT KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS FT DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI FT FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL FT IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI FT FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK FT QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD FT FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD FT RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS FT GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH FT GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK FT YFKDLMREI" FT misc_feature order(18421..18477,18874..18927,19012..19080,19123..19191, FT 19228..19296,20014..20073,20134..20202,20230..20298) FT /note="8 probable transmembrane helices predicted for FT pSAS.23 by TMHMM2.0 at aa 7-25, 158-175, 204-226, 241-263, FT 276-298, 538-557, 578-600 and 610-632" FT CDS 20341..20646 FT /transl_table=11 FT /locus_tag="pSAS24" FT /product="putative exported protein" FT /note="Similar to Bacillus subtilis hypothetical protein FT yxea precursor YxeA or bsu39620 SWALL:YXEA_BACSU FT (SWALL:P54940) (115 aa) fasta scores: E(): 0.012, 30.69% id FT in 101 aa" FT /protein_id="CAG43282.1" FT /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK FT DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKIFLAF" FT misc_feature 20341..20412 FT /note="Signal peptide predicted for pSAS.24 by SignalP 2.0 FT HMM (Signal peptide probabilty 0.953) with cleavage site FT probability 0.581 between residues 24 and 25" XX SQ Sequence 20652 BP; 7507 A; 2741 C; 3117 G; 7287 T; 0 other; ggtgaccgat agtgataaaa cttgaaaatg tttttgtaaa aaaaggtaat aaaaacattt 60 tagacggatg taactttaat tttgaaaaag gaaaatcata tgctttagtt ggagaaagtg 120 gcgctggaaa atctacttta cttaacataa tcgctgggtt cgaagatgta tcacaaggaa 180 gcatatatat tgaagataag ttactaaaaa agaaagtcga tttttataga tatacgttag 240 ggtatttgtt tcaaaatttt gctttacttg aaaatcaaac aatcagtcaa aatttagatt 300 tagccctaaa gttcaaaaag aataaaaaag ataatatgaa cttaaagaaa gaagtattaa 360 aaaaggtcgg cctagattta gatatcaaga gaattgttag ttctctaagc ggtggtgaac 420 aacaaagagt agcattggca agattaatat taaaagatcc gaaaatcatt ttagctgatg 480 aacctactgg gtcactagat acgaaaaatg gaaaaatagt tattgattta ttattgaagc 540 tacttgatga aaataaaact atgattgtcg taacgcatga tttagagctt gcgaaacgat 600 ttgatgtcat agtcaatatt tctgagctta gaagttaaga aatattgata ggtatagcca 660 tacatagaga aaaaacaata tgaaatatga ttagtcatta cttttgagtt ttttaattta 720 aggactggat tttccagtcc ttttttatga tgataaaaaa caagcctcgc agagcacacg 780 tttttaatga cttatttggt ttttaataag tctagtatgt taaaactttt ctgtgtttag 840 gtgttataat catatgcagt ggaagttgat ttattatgtc ttattcaatt attcgagttg 900 ttaaagtgaa atcaaaaaca aatacaagag gtattcaaag acatattcaa agagaaaata 960 aaaattatga aaatatagat attgatttaa gtaagtcata tttaaactat gatttagtca 1020 atgatactaa gtttgatttt aataaaaaaa ttgatgagaa aatcgaaaaa aattataaag 1080 ggaaacgcaa aattagaaca gatgccatta aacatattga tggattaatt acatcagata 1140 atgtgttttt taatcaatta tctgaagaag agacaaaata gttttttaag catgctaaag 1200 cgttcttgga agaagaatac ggtaaagaaa atcttttata tgcgacagta catatggatg 1260 agataacacc acatatgcat tatggcgtag ttcctattac taaagatgga cgtttaagtg 1320 cgaaagaagt tgtaggtaat aaaaaagcat taaccgaatt ccaagataga ttcaatacgt 1380 atattaataa gcaaggctat gatttaaaac gaggaatttc tagacaactt acaaaagaaa 1440 aacatgatca agttagccgt tataaacaaa aaacagaata tcataaacaa atgcatatgc 1500 gctagaagca aatagaggat tatttaaagc aagaaaatga taaattgata caaaactacc 1560 aaaaatcatt agatacgctt aaaaagcttc tgagtgtcat gtatgaaatt aaaactaaaa 1620 atgtaggtgg atggtttcat aaagagaagc aagaaacagg caatattgta attactaaaa 1680 ctgattttga aaaatacact aaacaaatta aagcagcaca gatgatatta gatgattatg 1740 aatgtattaa aagtgggaag tcattaaaaa aagcagagaa acaaaatgaa agtttagtta 1800 atgaattgac tagtgtccat atggaaaatg aaaagctggt tgaagaattt aatgatttag 1860 cacaacgtta taattatttg ttgtcagaga atgaaaagaa agataaagaa ctaaattata 1920 cgcttaagtt attcaatcaa gtttttaaaa tcattaaaag tatgatgaaa gaagagcgtt 1980 atcatacact aataaatcat attgataatc atttagacaa ttctaaaata cgtgaagtaa 2040 tgacgataga taacaatgat gaacagttct ttaagaagaa atatcaagca caagaacgag 2100 aaatcatttt taaagaagat agagaagatg gttatacatt ataaaaatag atatttaaat 2160 gcatttgttt gaggttaatt attttttagg atgaaaaata ggtagccccc tcgaactttt 2220 taatatattt ttataaatct ttttaaaaac ctttttaggg gcttgttcag ccctagagca 2280 acaagggatt tcaatgccta aaggagtgtt tttgccacat aaaaggagtg tttttgccac 2340 ataaaaggag tgtttttgcc acataaaagg agtgtttttg ccacataaaa ggagtgtttt 2400 aaaaagttgt actcctttta tggtttgaca ttgataataa agtagtttat tgtgaataaa 2460 agttttcaaa tgggagtgta tattcaaacg atactccttt aggttcgtaa taaagggggc 2520 tgtatcatga caggagagac agttgtatat aaaaatgaaa tgaatttagt tccattaagg 2580 cgatttacag ctactgaaat taatttattt tttgcgatgt gtaataaatt aaaagagcaa 2640 gatactaata cattacgttt gtcttttgat gaattaaaaa aattaagtaa ttatagtcca 2700 gaaacacgta atattaatag atttgctaat gatttagata atgtgtataa aaagatgttg 2760 aatttaacca ttagatatga agatgatgat gtaatagaaa gattcgtttt attcaatcat 2820 tatagaatcc ataagcgaga acaatatcta gaaatatcaa cttcttcaaa tttaaaacac 2880 atattgaatt caattactaa taattttaca aaatttgaat taaaagaaat gactaggctt 2940 aaatctacat attctaaaaa tatgtttaga ttacttaaac aatacaaaca tacaggctat 3000 ttaaaaattc acatcgatga ttttaaaaat cgtttagata tacctaaatc ttatcgtatg 3060 actgatatta acaaaaatgt ttttaaacct attattattg aattaggttc tatatttaat 3120 aatcttacta tcaataaaat caaagcgaaa aaagggcgta aaattgaatg gatagagttc 3180 acatttgacg ctgagaaacg cattcataac aaacgacaac ctcaaatgtc caagatagat 3240 aaatcaagac aatatgtgag gcgtgaaaag acgcctaaat ggttagaaga acggtcatat 3300 gaaaagcaac ctcaaaaaga ttacgaccca caattagaga aagaacgaga ggatttttta 3360 aaacaacttg aactgaattg ggaataacgc aacttttcgt tgcgagttgg ttaactaaaa 3420 gtatcgtgac ttttcaatta ttttaattat agtttgagtt aaggagtgat tttatgaaat 3480 tagctgaagc tataaaagaa cagagagaat taaaaggatg gtctcaagag gaattagcca 3540 atatattaaa ggtttccaga caaagtgttt ctaaatggga aagtgccaaa aactatcctt 3600 ccttagatat tttgattgct atgagcgatt tattcggaat ctctttagag catttaatta 3660 aaggagatgc acgttttaaa aaagttattt tagagggaaa ttatagagag agtaacaggg 3720 taccttccat agttgatttt ctttatgatt tttggtggct tttctttcct gttgccggat 3780 ttttagtttg ggccattcat tcgttcatgg gttgatttta aaatcaaaat ttaatgcaca 3840 tcaggcaata tgaaaaagga aagtattttt acgacacctt gaaatgtatt gataagaata 3900 gaagagttta caaaaggcaa tatgaaatat atactaaaag taacaagaat ttcgtttgta 3960 aacgcaccaa accccgaact attcgccgta gttcggggtt ttttggtgaa ttggctttaa 4020 tcgtctatat tattttttat cacgtttatg gagctaataa ccaaataacg taacgagaca 4080 accactgatt gccgttgtca tgatgtgaac aagaatttca ttcataaatc gcacctcctc 4140 tctacgccga attgacatct gagagataga cgaccttcac attataacaa atctctcctt 4200 ccatgaaata acatgaatat taataaatgt tgttatatat taaagataaa taaatataag 4260 ggtgggattg ataatgtttg gcgatcaaag acaagaagct acaaaatatg tcattaaaga 4320 aggttatcaa gatatttatt tcctcaacaa aaacggggaa tggtattatt tcgaagtaag 4380 aagtgtctgg cgtggcaaac atattattag agttaaagat ggtttgttag gttggagaaa 4440 agaaatagta actgaataaa gatacaaaat acttgccaat gcaaaagaat gaaaacgcgt 4500 caaatcaacg atgtataaaa gatgcataaa agataatgaa agtaaaaagc cataaatgcc 4560 gtcatatagg catttatggc tttttgtttg gttaatataa tcgatgatta tcagaagttc 4620 aattcacata aaaaagcacg gctaatttat gaacttgtcg taccttttta attaaaatta 4680 gttaccaaat agacgactcc aaaacccctt tttagcttcc gtttcttcac tcttttcttc 4740 tatatcacta tcattgtcat ttggattagc tgaaacgtct tctctttgtg cttcttcacc 4800 ttgtgtactt tcgtcgcttt gacttccaga tatatcctta tattgaatct ctgtttcttt 4860 agtttcttcc tgatctcggt taatatttac aggactagta gtatatgttt tttcttgtgc 4920 atcaacgttt tgtctagcgt tagtagatgt atcaaaggga taattaaggt gtttttcttt 4980 ttctaattga ttttctaatt tttgaatctt tttattactc tctaatgcca gaacttgctg 5040 attctcaagc aattttgatc tttcttgcaa ctcttttctg taaaattcaa ttgttgtttc 5100 atgacgactt tcttgttttt caatttgatt atttaaagta ttaatctgat ttttaagtat 5160 ttctactatt tgagcatcgc ttttagattc aaaattaata ttatccgact cttttttttg 5220 tttgtcttca gtagcacttt caaaaccata tttcttttta ttttcattta ctctttgaat 5280 gattttttct atatcggtat cttctttaat aaacgaagtg ttttcctgtt ttattgtctc 5340 tatattcaat ctcttaatat tattaaaaat cgtttgctta ctaaccccta attcttcact 5400 caaccttttt acacttttca tatcatcaac cctttataaa atgtttataa acccttagta 5460 aaatattata agcttataac gattaataat aaagtaatcg acaaataaaa atataaattc 5520 gccaaccttt ttatcaaggg tttgtaaagg gtttatggtg tttatcaaag gtttataaac 5580 ctatattttc ccttttttaa gtttattcaa agtgctataa taaccttaat ataacaaaaa 5640 ccccaactac tggtaatagt cggggtttgg taataaaacg tttagaaact gtttatttat 5700 ataattttcg atacttagat tatataatga ataatatctt agtgcaagca cctagattat 5760 taaataaaca caaaaaatgt aagcgtttta ttacctctta ccaaactaaa attcggaaag 5820 aggtttttat tatgtcaatt aaactttaca acataaatga ggaatacaga gaaaagtttt 5880 atcaaatacc gaaagtattt tttacaaatc ctaaatatat caagttatcc aatgatgcaa 5940 aaatggcatg gggtattctt agagatcgtt tagatttatc aattaaaaat ggttgggttg 6000 attctaaaac aggtaatatt tatttttatt acaaaaatga aaacccacaa aatatattaa 6060 attgtaaaaa agataaagta attaaaataa agaaagaact taattgaagc cgaattacta 6120 ctacaagaaa gacaaggttt aaataaaaca aataaacttt atttagttaa accagaagta 6180 acagaagatg atatttataa aattcaaaag caagaaaaca ctgttgagag cagcaatggc 6240 aaggaagtcg gaaaaaccga cttcaagaag tcgataaaac agaatctaat gatactgaac 6300 ttagctatac tgaatctaac aatactgaaa taaatgatat gaatgacttg aatgatatag 6360 agtccgaaaa tgaaattagt aatcattcaa gtcaatgcaa ggttatttaa tgcaaacaat 6420 tttaactgaa tttgaagaaa cacatgcact ttatatgcgc cgtaaaaaca tgaaacaata 6480 taatatcttt aatcaatcat tcaaatgatt ttataacaat caaagataca tcaaaatatt 6540 tcgttaaatc atctgacttt ttaactgata gcgatgaaaa aatcaagaaa ttgtcagtga 6600 tctagaaaaa gatttgtatc gaaaacgtat gttaagttat ggtggattgc ttaaataaaa 6660 acataaaatt ttaaacttag acgatgctga agatggcaat ttaattaata caagtgatga 6720 agataaaata gcaaatgaag aagaaaaggc acattcgatt acggcaattt tgaaaagcaa 6780 aattattatt taaggcatta gtgataactg atgtcttttt ttattacttt tttgtaaaaa 6840 aagtgctgtc ttatttttgt gacaaatgct gtatgtagtg tcacaaaagt gtgacacttg 6900 agctttttat gaccccacac aaaaacattt tcgaagcctt gaaatatctg gctttgccag 6960 atcccccatt cattgatggg gacaaatttc ccttatgctc ttacggagat tttagagtta 7020 aattaaaatt tctaagtttt tgataagaaa agccctgcag gaatttagaa gaacgtgggg 7080 aagttttaaa gaattttatg ctaattttta atttgcatgt aaatgggcag tgtctaaaaa 7140 atagttattg tttttgctca aatttttgtt tttgaaaata gcatatattt atttggctca 7200 tatttgcgtt ttaaaagcgt atataagttt tagataaaac tatatgaatc acccctaaat 7260 ctttaaaatg ccctcttaaa attcaaaata agggcataaa aaataaaagc ccccccagat 7320 aaagtgaggt agctttttct ttcgacgccg tatcgtcgat gtaattcaaa aaagttatgg 7380 gctataaatc tacatcactt tttcaagaat gtagtagcaa tattcaacgc aaattaattt 7440 ttataactgc tcggaatatc tcaagccgtt tctttaattt tgaaataaaa aaatcgacga 7500 aggtcgatta cgtttttgca catgtcgatt tatccgacgt ataagtgcgc ccttacggga 7560 tttaactaga ttatgacgac gaattttaga cctgtaaagc aataacaatt aggtctacca 7620 ggtagataaa gtgtacaaag tagaaccgaa aaagttattt attttgaaga tttttcgtag 7680 atataatttg ctaataaact tcctaaaact gaataaagaa aaatagttaa actcatgtaa 7740 tcactccata attctgaagt gtctccatgt ctacgcatag acacgcattt atgccgagaa 7800 aatttattgt gcgttgagaa gaacccttaa ctaaacttgc agacgaatgt cggcatagcg 7860 tgagctatta agccgaccat tcgacaagtt ttgggattgt taagggttcc gaggctcaac 7920 gtcaataaag caattggaat aaagcaccta tgattattta aaaatataag ttataataca 7980 ttcaaaaata tttttgaatg aggtgtatta tgattcaaac ggttgttgct gctgctgttc 8040 tttatattgc tacagcagtt gatttattag taattttatt aatatttttt gctagagcaa 8100 agactagaaa agaatataga aatatttatg ttggtcaata tttagggtct attattttaa 8160 tattagttag tttgttttta gcttttgtat taaattatgt tccagaaaag tggatattag 8220 gtttattggg tttaatacca atttatcttg gaattaaagt ggctatttat gatgattgtg 8280 aaggagaaaa gagagctaaa aaagaattga atgaaaaagg attgtctaaa ttagttggta 8340 cggttgcaat tgttacgata gcaagttgtg gcgccgataa tattggttta tttgttcctt 8400 attttgtgac attaagtgtt actaatttat tacttactct gtttgtcttt ttaattttaa 8460 ttttcttctt ggtgtttact gcacaaaaat tagctaatat cccaggaatt ggagaaattg 8520 ttgagaaatt tagtcgttgg attatggcta tcatttatat agctttaggt ttatttatta 8580 ttattgaaaa tgacactatt caaacaattt taggatttat attttaattt agggtgtgat 8640 tttatatgag ttatgaaaat acttgtgatg tgatctgtgt acatgaggat aaagttaaca 8700 atgctttaag ttttttagaa gatgataaat ctaagaaatt acttaacatt ttagaaaaaa 8760 tttgtgatga gaagaaattg aaaatcatat tatctttgat taaagaagat gagttgtgtg 8820 tttgtgatat ttctttgata ttgaaaatga gtgttgcttc aacttcacat catttaaggc 8880 ttttatataa aaatgaggta cttgattttt ataaagatgg aaagatggca tattatttta 8940 ttaaagacga tgaaataaga gagtttttct ctaaaaatca ggagggtttt tgaaacgagt 9000 gaaacgagtt tcttcttgtc ttgatactat aaaaaacaaa gttatataac ataagacgac 9060 ttatcggaag ttatctatca tcgtatattc ggagatgata taatatacag gtcgcctatc 9120 tctcaggcgt caattcagac gcagagagga ggtgcattac tttgctaatc attttcgttc 9180 acatcatagc accagtcatc agtggctgtg ctgttgcgta ttatacttat tggcttagta 9240 aacgcaacaa ataattaata ggcgacttta taagccagca tcgaaaaaat ccccacacta 9300 ttgcagtagt gtggggattg gtgcattaat tgttaatcat tttcgttatc tagattataa 9360 cactggttag tagaggaatg caaaaagata atattttaag tagtcttttt ggatattatc 9420 atatgatata tttatgtaaa ttgatttttt attaaaggag catatatgag taatactaaa 9480 attaaatatc aaatttttat ttcatcaact tatttagatt taatagaaga gcgtcaagca 9540 gtagtagaag cagttttaaa cagtaataat attccagcag gtatggaact ttttaactca 9600 agtagtaaaa gccaatggga aataataaca caatggatca atgaatcaga tgcagtgata 9660 ttattgttag gttctagtta tggaacaatt gatagtgaag aaaatattag ctatacagaa 9720 atggagtaca attttgctaa atctttaggt aaacctatat atgtttttga gatgaataaa 9780 gcatctaccg aatccgcgga aaagtctaaa aaattagatg agtttaagac aaaagttaaa 9840 gaaaatagac taattagtcc aaatattaat gatatttcgg aattaaaaag taaagtatct 9900 cagactttaa tacaaagtgt acctagtgaa attaaagaaa atagtggatg gataagagta 9960 caggattgtg gtaaaaatgt aaaaaaaata aaaaaattgg atgctataca aagagattta 10020 gatacatatg attttttgaa agaagaactt tataaaatat tcatgtatgt aaaaactttt 10080 tatttccagc acacaccatt tgatgatagt tatattgaag atatagaaaa ctacctgcta 10140 aattatgaaa ataatccaca taaatcattc atgaaaataa aagtttatga ttataaaaac 10200 tttttaaaac atttaaaaaa catattgatt ttaattaaac aaggaaaaaa gccattagct 10260 caagccggtc attttaaatt aagcttttac gaagaggatt taaaattgtt acaaaaaaat 10320 tataaggcta cgctagtcga tattaaagga ttgaataaac atttttataa ggaatgtgat 10380 aggtgcttaa aagcatataa tatttttatt gagggtataa ataaacattt atatgaatga 10440 tataagtaca ccctaatcat acatatataa ttgttgtcac tttactcgat atatgagaat 10500 gactttacct aataaatgag aaaagcaacg cactatatta aaatgcgttg ctttttctat 10560 tatcgaatac ctataactaa actatctcta gctgtttcta caacttctgt acttatttta 10620 tctaaaccat ttatatccat aattctgtct atctgcgcaa ataaacgatg aatcagccta 10680 aaattccctt ttgtaatttt aataatcgtc gttattgcct cataatcagt aaagtcttcg 10740 agttttaaat caaatcctaa gtcttgacac ttatattcta atatatgatg cgtctcatct 10800 ttacttagat tatcaaattc atgcgcaaaa cctatacgcg aatatagttg aggataacga 10860 gatagttttt tctctatacc cggcatacca ataaatatca ttgctaaatt atgttcatca 10920 taaatagctc ttaattgctc taaatgttgc actttaaggc gatcaatctc atctactata 10980 attaaatcaa tatatttaaa catatcacta taatgtttac tatgttcatg tccatactta 11040 tttacaacat gtaatttttg acccaaatca atgattgcag taatactata taaatcattc 11100 cttaatttag tttgtttttc tgctggtgca ttataaaata ttttatccac cgatagtatg 11160 tcatcggtcg ttttactagc taaatcttcc caacctctat ggttaacttg tttttcaata 11220 gtatcccaat ttgtgtaata cctagacgat aaagtttttc cgacaccagg ttgaccataa 11280 catataccaa tatactgata tttaatacaa gcatcacaaa attcagcaaa gcgtttatac 11340 tctttcgttt caatgaaatt ttgattttta gtcattacga tatctcttta attttttgac 11400 gtttttagtc gtttcttgag gtgaataacc acaatttttt tcttccttaa ttaattccgt 11460 ggtacttgga ctagcaatat tttgttttaa gtgttttctt ctctgactac gagcgtgctg 11520 tatgtctttt atatctatgg agtaatcagc tatttctgga catatagcag tacaaagaaa 11580 ttcatctcta taaaacacac gtatttcagc catatcattt gggttataac gaatcagtac 11640 atattcacct acataagctt ctagattagt attactgtat cgaaaacctt gaaaatgaat 11700 gccatctgaa tgaacttttc tagatttagg tatttccaac aataataaat caagttgttc 11760 taagctactt ggcatattag ggaaaaagtg attactattc caatgactga taggcgtggt 11820 atgaatagta ctatgttctt tttgattata atcttcaatt aaaaagtaac gtaatttttc 11880 ttcaaaattg tgaaaattta ttagattact aggagtatca ttattgttta tatatccagg 11940 cagttgttct aagaaagttt gattaactgt ctgaaagaat cgttctattt ttccacgacc 12000 acgaggaaca ccaactttcg aaaacatcaa gttaattttc aaatcaatag cgacttgttc 12060 catatgatgt gaagtaaagt cacttccatg atctgtgtag aatttttcgg gtataccaca 12120 cactggccaa ttggtatcat ttttattcca tattgcttgg tgtagtgtta atgctgtgtt 12180 ttgagcattg gggggcatca aaacttaaga aatagcctgc aattgcacga ctatagtcat 12240 ccataataat agttagccat ggtctattga tattaccttt ttggtctaat ataaagatat 12300 ctagcaaagt atggtctgct tgccatattt cattgggtct cgaggattct ctaatttgta 12360 ttaaatcgta cttattttga tagtattttt caccctgatg agagaaatca ataacagact 12420 taggcatcgc tttaataaca ctataaattt gtttataact tggtatatca aaattatttt 12480 ctttacagta attagtgatt ttcctatgaa ttgtggctat actatttctt ttattcataa 12540 gcgcaaggcg ttcaatttca tcaattatct ttggttctat tttacgtgtt cctttatcag 12600 ctcttgtcgc atagattagt cctttaagtc cttgttcttt atatgactta ttccacctat 12660 aaagggtgct taacgcaatc cctttacttt tagaaatact tgataaagat tgttcgccta 12720 ggataaaagg ttttataata ttgtactttt gagtagcttt tttacgtttg gcttcagaaa 12780 aattagttag atacttctcc ttgtttttca tcttccttgt cctccaattt attgaggtaa 12840 cgataaattg ttgtacgtga aacatgccac tgttctgcaa ttgtcttaat tggcgtgcca 12900 ttatcataaa gggtcttaag taagtttaaa tctttttgat ttaatttttc tggtctgcct 12960 ccatatcggc ctctagcacg tgctgcaatt cggccagctg aagaacgctc taaaattaaa 13020 ttacgttcaa attctgcaaa tgctgcgaat aaatgaaata ataattgtcc agttgaactt 13080 gatttatcca ttgttatatt ttcttctaaa ctatggaaac ttacacctcg ttcgttaagc 13140 tcattaacta atgtgattaa atcctccata ttacgtccca atctatctaa tctccaaaca 13200 acaatcgtat ctcctgacct cgcaaattca attgctttat ctaaaccagg tcttttactt 13260 tttgaaccac tcatatggtc attaaatatc ttttcacaac catatgtatt taatcgatcc 13320 tcctgtaaat ttaaattttg taatccagtt gaaactcttg cataacctat tttcaaaata 13380 caaacctcct atatacatct tcagtgtacc ataacttaat tacaaggtct tttatgaaca 13440 tagatttttt gttgggtttt tgaacacata atcctatgta attcttactt atttagacat 13500 aaaagaagag tgtacagaaa atggttattt tctgtacact ctcatcttta ataatatatt 13560 ttaaatgtca ttaacaaaac taatttaata agagtcaagc atagtttaca tatgtattat 13620 gtttgaaaca tacctctaat tactttttac taatatcatt taaaatatct cgcaattctt 13680 caatttcttt gttatttaat tcttcatttt tcgcaaaatt caacactaaa cttttcatat 13740 cccctccata cagtttatta agaaaggttt tagcagtttt cattttaata tcgtcttctt 13800 taatatttga tgagtaaaaa taaatatttt ctgatttgta tcgttttata atctcttttt 13860 tatatagtct tgtgattaat gttctaatcg ttttatcact aacttcttta tatttttgaa 13920 tttcaactac aatttcatta gctgatactg attttttatc ccatattata ttcataacat 13980 cccattcagc catagatatt tcaacttgct tattggccat ttaaaacacc cattcctttt 14040 aatatttttt cacttattaa ttcagcattt ttcccagacg ggtttccatc tgataaatgt 14100 gtagcaaaat aatacttatc atgatttgta attacgtaac ctacaaacca cccattatta 14160 tacttcccgt ttactatacc tgtacctgtt ttcccataca gttcatactt ttcatttttc 14220 ttaatcaata atgaagaaga taattgattc tttgcttttt tactaaaatg gttattttgt 14280 tccatcatat ttttaaaaac tattacttgt tcaagattag atattttcaa actatcttcc 14340 atccaatagc ttttataact tcccaaattt tcattaccat aatttaattg cttgagttga 14400 gccgcagtat agttctttgg tatttgatta ctaatacgtt cgaagtacca attaactgaa 14460 ttttgcattg ctgtatttaa atcttgttcc ttattccaag actcgaaaag ataatgttta 14520 tgattccatg acatacgtga atttttatca cttataatat gacggtcaag tccaaacagt 14580 gctaaataaa ttttgtaagt tgaatcagga gaatatcttt ttctactttc tttttcatta 14640 taaatatagt atttgtcttt cttcatgctg tacataacga aagatccact atttgaacca 14700 aaatttttac tttcatctaa aatttggtaa tcgctttgta agggcttttt ataattataa 14760 tcagttaagg attgccccat taaaaactgg ctttgaatta tcatgataaa aaaagtaaaa 14820 atacaaataa aaattagaat taattttgac tgctttttca gatcggcttc ttttatatta 14880 attaatctat ttttgagtaa tgatttttta ccattaaatg aatggcttaa tatatttttg 14940 ttgaaaaatg aggtttttaa tacggagtcc ataatagatt ccgcataagt tttaaattca 15000 tttttgttaa tattattaag gacaaatcta tcggcttcta cttcattgtc atgaattatc 15060 tttcttttta caatatgtac taatggattg taactcataa taatagagaa gatattaaag 15120 ataattaaat gtaaagtatc tctattttta gcatgagcat attcatgcag tattatatat 15180 tttagtcttt tatcaattac acttttaaaa tatgaactag gaatcaaaat aatatatttc 15240 ccataccaaa aagttattgg agattgaata gcctctgctt ttcgaatcac aatatttttt 15300 ttatattgat ggttgaaaag tatcgtatct attttatttt tttcattttc gtttagataa 15360 agtgactgtt tctttaaata tttaagatat aataaggctt tcaaaaattt aaaacttaaa 15420 ataataacta aaactatcca aataactgtg cagatattat caattgaatc ccaattaaac 15480 ttatggatat ctgttgcgaa ctcttgaaca ggtttggtgg taattatgtt atggttcaag 15540 tcgtgtgact tactttcaac tgtgggcgct tgattattca cattattaaa ttttataaaa 15600 gagaatttaa ttgggataaa aggaattaat ccagcaagca cagttagata ccaaacttta 15660 taatttaaac tataattaaa atagcgtttt aatatgtacc taaaaaagac taacaataga 15720 aatataaaac aaaagcttac tacactcatt attagtaatt tagccatttt gacaccttct 15780 ttcaaatatt tataataaac aattgacatc aatattacaa ttgtaatatt attgttttgt 15840 aaaaattaca actgtaatat cggagggttt attttgaaaa agttaatatt tttaattgta 15900 atggctttag ttttaagtgc atgtaattca aacagttcac atgccaaaga gttaaatgat 15960 ttagaaaaaa aatataatgc tcatattggt gtttatgctt tagatactaa aagtggtaag 16020 gaagtaaaat ttaattcaga taagagattt gcctatgctt cgacttcaaa agcgataaat 16080 agtgctattt tgttagaaca agtaccttat aataagttaa ataaaaaagt acatattaac 16140 aaagatgata tagttgctta ttctcctatt ttagaaaaat atgtaggaaa agacatcact 16200 ttaaaagaac ttattgaggc ttcaatgaca tatagtgata atacagcaaa caataaaatt 16260 ataaaagaaa tcggtggaat caaaaaagtt aaacaacgtc taaaagaact aggagataaa 16320 gtaacaaatc cagttagata tgagatagaa ttaaattact attcgccaaa gagcaaaaaa 16380 gatacttcaa cgcctgctgc tttcggcaag actttaaata aacttatcgc aaatggaaaa 16440 ttaagcaaaa aaaataaaaa tttcttactt gatttaatgt ttaataataa aaacggagac 16500 actttaatta aagatggtgt tccaaaagac tataaggttg ctgataaaag tggtcaagca 16560 ataacatatg cttctagaaa tgatgttgct tttgtttatc ctaagggcca atctgaacct 16620 attgttttag tcatttttac gaataaagac aataaaagtg ataagccaaa tgataagttg 16680 ataagtgaaa ccgccaagag tgtaatgaag gaattttaat attctaaatg cataataaat 16740 actgataaca tcttatagtt tgtattatat tttctattat cgttgacatg tataattttg 16800 atatcaaaac ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa 16860 caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag tttaattata 16920 ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt 16980 tataaggtta aataattctc atatatcaag caaagtgaca taattgttga atttatagta 17040 actgaaacgt atgattaggg tgtatattaa ttatgagatg gaaatcaatg attgttggct 17100 atgcgagagt atcatcgaac gatcaaaatt tagaaagaca acttgaaaat ttaaagacgt 17160 tttgtgctga aaaaatattt acagaaaaac agtctgggaa atctattgaa aatagaccag 17220 tacttcaaga agcacttaac tttttgagga tgggagatcg gtttgtagta gaatccattg 17280 attgtttagg tcgtaattat gatgaagtgg ttggcaatcc attactggat aagtttatga 17340 aagatttaat tattcaaata ttagcaatgg tttcagaaca agagagaaat gaaagtaaac 17400 gtcgacaagc gcaaggaatt caagttgcga aggaaaaagg cgtatataaa gggcgcccct 17460 tactttattc accaaatgct aaagatccac agaaacgtat tatttatcat cgcgttgtag 17520 aaatgttaga agaaggccaa tcaattagta aaattgcgaa agaagtaaat ataacaaggc 17580 agacgattta cagaattaaa aacgataatg atttgattta ataaactttg tttcaatgca 17640 actgagagct tctcacagcg tctcaaaaca gatgattata atctattatg agtttttaat 17700 aataagacaa gaaaagagag cgtaaatatg ctctctcttt ttctcattaa cgaacgttta 17760 taacgcaatc atgatgagag tattaataca gttaattatt tgaaatactt tagtttatat 17820 aatgttgaat tataggtagt gaaattgtat ttattataca ttgtcatatc gttgtttaat 17880 gatagttaat ttaaggataa tagaattaat attatatata gatgaacaaa acaagcaaca 17940 gagcagaaaa aacactttta gattattttt actcattctc tattttattt atttttgcta 18000 taataaataa ataatattaa atggaggtgt ttttatgaaa aagaaatttg tttctagttg 18060 tattgctagt acaatcttat tcggcacttt attaggtgta acttataaag ctgaagctgc 18120 aacagtacat gtagctggtg gtgtttggag ccatggtatt ggaaaacatt acgtatggtc 18180 ttattatagt cacaataaaa gaaatcatgg ttcaacagca gtagggaaat attcatcttt 18240 tagtggtgtt gctcgacctg gtgttcaatc taaggcatct gctccaaagg cttggggtgg 18300 aaacaaaaca ttttatagtc ttcactaatt acatatatta attaaggaga atagacagaa 18360 ttaagtaaac ttaattctgt ctattctctt gaaagaggca atatgaaaaa attcaaatta 18420 tttttagatt ttagtacttt attattaatt tcagggctat tatttttatt tttcttcaaa 18480 gaaaatgaag aaattatacc tgaaagttcc aatattttaa caatctctaa ttgggataaa 18540 tctaacagta aatcaaaagt gttagatgtc attgaatcag gcgccaaaaa tcaaaatata 18600 caaataatta aatccgttaa agattttgat aataaaaaag aattttttgt tttcaactca 18660 aaacgaaata attctgactt tatacgtaat aaaacatcac ttttaactcc atcagattta 18720 ttaaacagag agataaaagg aaagtattat attattggtg aacatttcaa tgtagaagag 18780 cttacgagtg aattaaaaaa agctggttta acatctgaag ttgaatacat aaatagaaac 18840 atattatttt ttgaattagt catagataat gatttattag tgcctttcat attcttaggc 18900 gtattatatc ttttgtactt cttatatgac agaggatata atttgaaatt ttatgctatt 18960 caaaagctac atggcttttc aactacacgc attatttttc ataatattca tataaaaatc 19020 atttattggc tagtacttac cgctattttc tttattgcta atatattaat cattcatata 19080 gccaatttag aattggattt cttaatgttt attcgtagat ttatcgtgtt gctttttgtt 19140 tttgcttgta ttactacatt gctgtggctg atttcgtata gtcttctttt gaagttaagt 19200 attccattaa tcttaaaagg gaaaaaagga tataagtttt caatatttat gggtaccttc 19260 accaaatgta tcatggtgct aatactatct tttttactgg cacaaaattt aaatgcttat 19320 acgaagttaa agaatatata tgactctgaa aaaatctggc aaaagttaga taattactat 19380 acattagaga tttctcctaa tattagaaat tcagaagaaa aacaaatttt agaaactaat 19440 atatacaatt taattaagaa aagcgaacaa cttggtggat tgcttttaaa gaataataat 19500 atagccaatc ccgataaaaa caattacatt cctgaaaatg gcaatgtctt ttttatcaat 19560 aacaattttc tgaatttcta taaaaatata aatcatgatt ttaaaatgat tgacaatcat 19620 tcggataaaa taaacgtgtt tattcctcca aggttacaat atcaaaaatc tgaaattcaa 19680 aaaaatcacc aaggatggat tgactttcaa aagcaacaaa acaaaaaagt agatgtgcaa 19740 gtactgtcaa aaaacgtatc tgttttttct tttgatcgca caactaattt gaaatttggg 19800 tatctagatt ctccaatagt aatgttgttg acacttgacg atttaacagg agatttttat 19860 ttagctgctg tttctcaagg tggatatcta tttaaagatt tagatacact taaacagttg 19920 cttaaagata atcacctaga agaagaaatt agcggtataa ctaattacaa ggattctgtg 19980 ttaaatgaat taaatgaaac gaaaacaaaa atgataataa caatagttac tattattatt 20040 aacgttttca ttcttttgat agctactgta tttgaaacaa ttcaatattt tagctggaat 20100 aaaaagcaac tattattaag aaaaattcat ggttattcat tattcagcag taatgtacgt 20160 tatttaacaa tttctatctt actttctatt atgttagctt acactacgca tattttattt 20220 ggttcaaaga tattactatt cataataatg tctattgcta ttattcaaca tttacttcaa 20280 attgcttata ttaaatatct tgaaaaatac tttaaagatt taatgaggga gatttaaaca 20340 atgaaaagaa ctttaattat tgtaacaatt gtactattaa tcatcatact tagtctgtta 20400 ttggttagaa atgagcattt agatcgtttt aacccactgt taaaagaaaa agtatcttat 20460 gccaaagttc caaaagatac ccaggactat agaaatataa ctgtatactc taaaaatgga 20520 gaaaaaaaga gttataaatt ggatttttta ggttatgatc ctacaaaaga atatgtgaaa 20580 gtaaatcata aagggaaata tgtgcgttcg atagaataca ttacaaaaat attcctagct 20640 ttttaaaatg ag 20652 //