ID   BG592789; SV 1; linear; mRNA; EST; PLN; 593 BP.
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AC   BG592789;
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DT   13-APR-2001 (Rel. 67, Created)
DT   10-MAR-2003 (Rel. 75, Last updated, Version 2)
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DE   EST491467 cSTS Solanum tuberosum cDNA clone cSTS2K20 5' sequence, mRNA
DE   sequence.
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KW   EST.
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OS   Solanum tuberosum (potato)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
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RN   [1]
RP   1-593
RA   van der Hoeven R., Bezzerides J., Sun H., Cho J., Chiemingo A., Bougri O.,
RA   Buell C.R., Ronning C., Tanksley S., Baker B.;
RT   "Generations of ESTs from sprouting potato eyes";
RL   Unpublished.
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DR   MD5; 5cbf8fa1421bebcf68b348e84aabde66.
DR   UNILIB; 23184; 8811.
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CC   Contact: Robin Buell
CC   The Institute for Genomic Research
CC   9712 Medical Center Dr, Rockville, MD 20850, USA
CC   Email: potato@tigr.org
CC   This clone can be obtained from the University of Arizona Genomics
CC   Institute. Orders can be made through URL:
CC   http://genome.arizona.edu/orders/
CC   Seq primer: M13F-R.
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FH   Key             Location/Qualifiers
FH
FT   source          1..593
FT                   /organism="Solanum tuberosum"
FT                   /lab_host="SOLR"
FT                   /cultivar="Kennebec"
FT                   /mol_type="mRNA"
FT                   /dev_stage="12-14 weeks post harvest"
FT                   /clone_lib="cSTS"
FT                   /clone="cSTS2K20"
FT                   /tissue_type="sprouting eyes from tubers"
FT                   /note="Vector: pBluescript SK(-); Site_1: EcoRI; Site_2:
FT                   XhoI; Various sizes of sprouting eyes (2mm to 15mm) were
FT                   taken from tubers. The tubers were incubated at 26C in the
FT                   dark for 2-3 weeks prior to sprouting. The eyes were frozen
FT                   in liquid nitrogen immediately upon removal from tubers."
FT                   /db_xref="taxon:4113"
FT                   /db_xref="UNILIB:23184"
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SQ   Sequence 593 BP; 176 A; 112 C; 126 G; 179 T; 0 other;
     ggaagctagg gcaagacgtc agcttcagag agtcttttga tgttgcagtt gcccgagcag        60
     ttgcagaaat gagaatttta gctgagtatt gccttcctct agtccgaact ggtggtatct       120
     ttgtagctgc aaaaggtcat gatcctcagg aggaagtgca aagagcagag agagcaatac       180
     aactgatggg agcatctcta ttgcaaattc gttgtgttga ttcccacagt aaacatggac       240
     agagaactgc cataatttgt ttgaaaggta aacctacccc caagaaatat cctcgagatc       300
     caggaactcc agctaagatt ccactttgag aggaatgttg tcatgcctcc gtatctccca       360
     attgtacagt taatgtataa cagagggaag tttgtatttt agtatccttt ctcttgagtt       420
     tagatgcaga aacactatac tcatggatgg ctagaagcag tactactagt tcctttgtta       480
     attgcttacc tcattatttg agaaggtaca ctacacagat ttggctttaa gtctatccat       540
     taagatgaat gtatattaca accacataat acttctatta ttttgtagct gaa              593
//