ID   B19391; SV 1; linear; genomic DNA; GSS; MUS; 51 BP.
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AC   B19391;
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DT   16-APR-1998 (Rel. 55, Created)
DT   12-JAN-2011 (Rel. 107, Last updated, Version 5)
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DE   pEMS730-E4 Flow Sorted Mouse Y Chromosome MboI Library Mus musculus genomic
DE   clone pEMS730-E4 5', genomic survey sequence.
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KW   GSS.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-51
RX   DOI; 10.1006/geno.1997.5176.
RX   PUBMED; 9545635.
RA   Bergstrom D.E., Grieco D.A., Sonti M.M., Fawcett J.J., Bell-Prince C.,
RA   Cram L.S., Narayanswami, S, Simpson E.M., Trent J.M.;
RT   "The mouse Y chromosome: enrichment, sizing, and cloning by bivariate flow
RT   cytometry";
RL   Genomics 48(3):304-313(1998).
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DR   MD5; c199b8f24ce87b927d803193dc87323c.
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CC   Contact: Simpson EM
CC   Simpson EM
CC   The Jackson Laboratory
CC   600 Main Street, Bar Harbor, ME 04609-1500, USA
CC   Tel: 207 288 6408
CC   Fax: 207 288 6073
CC   Email: ems@jax.org
CC   Suitable primers could not be selected from this clone.
CC   Insert Length: 51   Std Error: 5.00
CC   Plate: 730  row: E  column: 4
CC   Seq primer: T7
CC   Class: shotgun.
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FH   Key             Location/Qualifiers
FH
FT   source          1..51
FT                   /organism="Mus musculus"
FT                   /strain="C57BL/6J"
FT                   /mol_type="genomic DNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="Flow Sorted Mouse Y Chromosome MboI Library"
FT                   /clone="pEMS730-E4"
FT                   /cell_type="lymphokine activated killer cells"
FT                   /note="Organ: spleen; Vector: pBluescript; Site_1:
FT                   BamHI/MboI; Site_2: MboI/BamHI; Mouse Y Chromosomes (Strain
FT                   C57BL/6J) were isolated by bivariate flow cytometry.
FT                   Purified chromosomal DNA was digested with MboI. Fragments
FT                   of 400 to 1000 bp in length were size selected and cloned
FT                   into the BamHI site of pBluescript."
FT                   /db_xref="taxon:10090"
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SQ   Sequence 51 BP; 21 A; 8 C; 9 G; 13 T; 0 other;
     gatctgtctg acaaaaattc atgcctctga agaaagaaat taaagatgat c                 51
//