ID   B19362; SV 1; linear; genomic DNA; GSS; MUS; 500 BP.
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AC   B19362;
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DT   16-APR-1998 (Rel. 55, Created)
DT   12-JAN-2011 (Rel. 107, Last updated, Version 5)
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DE   pEMS729-9 Flow Sorted Mouse Y Chromosome MboI Library Mus musculus genomic
DE   clone pEMS729-9 5', genomic survey sequence.
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KW   GSS.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-500
RX   DOI; 10.1006/geno.1997.5176.
RX   PUBMED; 9545635.
RA   Bergstrom D.E., Grieco D.A., Sonti M.M., Fawcett J.J., Bell-Prince C.,
RA   Cram L.S., Narayanswami, S, Simpson E.M., Trent J.M.;
RT   "The mouse Y chromosome: enrichment, sizing, and cloning by bivariate flow
RT   cytometry";
RL   Genomics 48(3):304-313(1998).
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DR   MD5; a9ac6a944e86905dc943ce4c071876e0.
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CC   Contact: Simpson EM
CC   Simpson EM
CC   The Jackson Laboratory
CC   600 Main Street, Bar Harbor, ME 04609-1500, USA
CC   Tel: 207 288 6408
CC   Fax: 207 288 6073
CC   Email: ems@jax.org
CC   Insert Length: 900   Std Error: 135.00
CC   Seq primer: T7
CC   Class: shotgun.
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FH   Key             Location/Qualifiers
FH
FT   source          1..500
FT                   /organism="Mus musculus"
FT                   /strain="C57BL/6J"
FT                   /mol_type="genomic DNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="Flow Sorted Mouse Y Chromosome MboI Library"
FT                   /clone="pEMS729-9"
FT                   /cell_type="lymphokine activated killer cells"
FT                   /note="Organ: spleen; Vector: pBluescript; Site_1:
FT                   BamHI/MboI; Site_2: MboI/BamHI; Mouse Y Chromosomes (Strain
FT                   C57BL/6J) were isolated by bivariate flow cytometry.
FT                   Purified chromosomal DNA was digested with MboI. Fragments
FT                   of 400 to 1000 bp in length were size selected and cloned
FT                   into the BamHI site of pBluescript."
FT                   /db_xref="taxon:10090"
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SQ   Sequence 500 BP; 166 A; 87 C; 134 G; 113 T; 0 other;
     gatccccaat ggaggagtta gagacaggac tgaaggagct gaatgggttt gcaacccata        60
     ggaagagcaa caatatcaac cacccagatt ccccatagct cccagggact aatccaccaa       120
     ccaaagagaa cacatggagg gacccatgac tccaactgta tatgtagcag aagattacct       180
     tgtcagacat catttgtagg agaggccctt gttcctgtga cagcttgatg ccccattgtg       240
     gggtaatgcc cgggtgggga ggcaggagta ggtgggaggg taggggaata ccctcataga       300
     agcagaggga aggggcatgg gatagggtgt ttttggaggg gaaaccagga aaggggataa       360
     catttgaaat gtaaataaat aaaatatcta atgaaaaaat tcaaaaaatg tcacaaagtc       420
     agatgacaag ttgcaaaaca gggaaaatat ttgtatcagt cttttaaaac ttttttattg       480
     attgtgagtt ttgcaccatg                                                   500
//