ID   AQ840387; SV 1; linear; genomic DNA; GSS; PLN; 748 BP.
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AC   AQ840387;
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DT   07-SEP-1999 (Rel. 61, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   nbxb0058B10r CUGI Rice BAC Library Oryza sativa genomic clone nbxb0058B10r,
DE   genomic survey sequence.
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KW   GSS.
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OS   Oryza sativa (rice)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
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RN   [1]
RP   1-748
RA   Wing R.A., Dean R.A.;
RT   "A BAC End Sequencing Framework to Sequence the Rice Genome";
RL   Unpublished.
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DR   MD5; d443d54f8ac0b4c825c6c601c8cd65d5.
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CC   On Feb 19, 1999 this sequence version replaced gi:4143605.
CC   Contact: Wing RA
CC   Clemson University Genomics Institute
CC   Clemson University
CC   100 Jordan Hall, Clemson, SC 29634, USA
CC   Tel: 864 656 7288
CC   Fax: 864 656 4293
CC   Email: rwing@clemson.edu
CC   Seq primer: GGAAACAGCTATGACCATG
CC   Class: BAC ends
CC   High quality sequence stop: 202.
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FH   Key             Location/Qualifiers
FH
FT   source          1..748
FT                   /organism="Oryza sativa"
FT                   /lab_host="E. coli DH10B"
FT                   /strain="Japonica"
FT                   /cultivar="Nipponbare"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="CUGI Rice BAC Library"
FT                   /clone="nbxb0058B10r"
FT                   /tissue_type="Leaf"
FT                   /note="Vector: pBeloBAC11; Site_1: HindIII; Site_2:
FT                   HindIII; Rice is one of two most popular grains in the
FT                   world. Half of the world  population especially those
FT                   inhabiting highly populated areas of the  humid tropics and
FT                   subtropics, rely on rice as their primary source of
FT                   carbohydrate. Monocotyledonous rice is a diploid plant
FT                   (2n=24) with a  haploid genome equivalent of 431 Mbp
FT                   (Arumuganathan and Earle, 1991).  The relatively small
FT                   genome of rice, three times larger than that of
FT                   Arabidopsis, makes it suitable for genomic studies. In
FT                   order to  facilitate positional cloning, physical mapping
FT                   and genome sequencing  of rice, we have constructed a BAC
FT                   library from Oryza sativa, Nipponbare  variety. The library
FT                   contains 36,864 clones with an average insert size of 128.5
FT                   Kb providing 10.9 haploid genome equivalents. The deep 
FT                   coverage allows the isolation a particular sequence with a
FT                   probability  of 99.9 %. Two high density filters, each
FT                   containing 18,432 clones  (doubly spotted), represent the
FT                   whole library for colony screening."
FT                   /db_xref="taxon:4530"
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SQ   Sequence 748 BP; 194 A; 157 C; 191 G; 205 T; 1 other;
     cgtccatgtc aatcctcgca aagagattct tagtctttga gcggaatgct gaagtagtcc        60
     agcggncaag ggacagtgat acaagttggt ggtcagtgct gtcatagagc acctgtgtat       120
     tgccccacag catgtcaagg ctgtcggtca tgtcgctgat tactggggag gtggctagga       180
     tgagatacaa agctgccatg gacatggagg gtaggagttg agccctaggt tgccccatgg       240
     cttttgctaa tgcgaagtgc ctaagtgagt aggcagactg tggagtgtgc tgtgttgaaa       300
     tgttgtagac tttatataga ctcatgtgaa gaggaaacca ggtgttcaag tctaaacaag       360
     atctaggtct gacatactat taccttagat tcttggaagg tgtatgaact ggacatcatt       420
     ggctgacatg aagaagtggg cataaaaaaa atgataaaaa ttgggctgaa ccatagcttg       480
     agcaggaatg atccagtctg gattctgtac tctggagttg acttactggc tctgtcatcc       540
     aaccaatttt cacgaccgcc ccatggtggc tttttgacaa actcacctgg ccttaagcaa       600
     agagaaagct tatggttaaa aggtttaagt cccaatgccg tgccggtatt ggacggtgaa       660
     tcaccctcct tacctcactt ttgtaacgtg tactttcctt ttgttcaaca cagctttcga       720
     tgaccaccca ctccgtcatc tcgcgttc                                          748
//