ID   AQ329329; SV 1; linear; genomic DNA; GSS; PLN; 662 BP.
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AC   AQ329329;
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DT   11-JAN-1999 (Rel. 58, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   nbxb0044F14f CUGI Rice BAC Library Oryza sativa genomic clone nbxb0044F14f,
DE   genomic survey sequence.
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KW   GSS.
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OS   Oryza sativa (rice)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
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RN   [1]
RP   1-662
RA   Wing R.A., Dean R.A.;
RT   "A BAC End Sequencing Framework to Sequence the Rice Genome";
RL   Unpublished.
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DR   MD5; c3404d3ae0f464b52d84838876b3b9d7.
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CC   Contact: Wing RA
CC   Clemson University Genomics Institute
CC   Clemson University
CC   100 Jordan Hall, Clemson, SC 29634, USA
CC   Tel: 864 656 7288
CC   Fax: 864 656 4293
CC   Email: rwing@clemson.edu
CC   Seq primer: TAATACGACTCACTATAGGG
CC   Class: BAC ends
CC   High quality sequence stop: 426.
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FH   Key             Location/Qualifiers
FH
FT   source          1..662
FT                   /organism="Oryza sativa"
FT                   /lab_host="E. coli DH10B"
FT                   /strain="Japonica"
FT                   /cultivar="Nipponbare"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="CUGI Rice BAC Library"
FT                   /clone="nbxb0044F14f"
FT                   /tissue_type="Leaf"
FT                   /note="Vector: pBeloBAC11; Site_1: HindIII; Site_2:
FT                   HindIII; Rice is one of two most popular grains in the
FT                   world. Half of the world  population especially those
FT                   inhabiting highly populated areas of the  humid tropics and
FT                   subtropics, rely on rice as their primary source of
FT                   carbohydrate. Monocotyledonous rice is a diploid plant
FT                   (2n=24) with a  haploid genome equivalent of 431 Mbp
FT                   (Arumuganathan and Earle, 1991).  The relatively small
FT                   genome of rice, three times larger than that of
FT                   Arabidopsis, makes it suitable for genomic studies. In
FT                   order to  facilitate positional cloning, physical mapping
FT                   and genome sequencing  of rice, we have constructed a BAC
FT                   library from Oryza sativa, Nipponbare  variety. The library
FT                   contains 36,864 clones with an average insert size of 128.5
FT                   Kb providing 10.9 haploid genome equivalents. The deep 
FT                   coverage allows the isolation a particular sequence with a
FT                   probability  of 99.9 %. Two high density filters, each
FT                   containing 18,432 clones  (doubly spotted), represent the
FT                   whole library for colony screening."
FT                   /db_xref="taxon:4530"
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SQ   Sequence 662 BP; 209 A; 142 C; 111 G; 199 T; 1 other;
     ccagtcgcaa taatcctgtc aacctgcctg cccgagtatg catgagttga aggcatcaag        60
     cactttcttg aaatatcctt taatgtcaaa taagaggctt ctctttccac caaggccatt       120
     accctataag acaaaaaaaa tcttgtttca gctccatgag gattcttaaa tataaacact       180
     acatactgtc tcataatcat ttactctgca catgacaaga tagaacaaaa caatttaaaa       240
     agaacagaat atgaaagaaa actgtagcaa tataaatata aatgactaaa aaactaaaaa       300
     tgcctaggta caaatagttg tgacctcgat ccccagtaat tatgtagaag cgactcacct       360
     cttctctgtt tcgctatcca ctctaggaaa tgaactttgg ggtatcttcg tagaagtaga       420
     tgatgaaggc atagttacca acagttttcc ctgctctttt gcaggttttg taatgcctgt       480
     agaagcatcc ttgagcagac atggatcagg ctgtggtgta tctttcaaga acctggcttt       540
     tttcttatcg ggtttgacaa caanattgct cagatgaact cctagcacta atctttctgg       600
     ctcgatcact ttcttgaatg ccaaccatat tctgcagttt caaaattctg tcgattctct       660
     cg                                                                      662
//