ID   AQ329318; SV 1; linear; genomic DNA; GSS; PLN; 666 BP.
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AC   AQ329318;
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DT   11-JAN-1999 (Rel. 58, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   nbxb0044F02f CUGI Rice BAC Library Oryza sativa genomic clone nbxb0044F02f,
DE   genomic survey sequence.
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KW   GSS.
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OS   Oryza sativa (rice)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
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RN   [1]
RP   1-666
RA   Wing R.A., Dean R.A.;
RT   "A BAC End Sequencing Framework to Sequence the Rice Genome";
RL   Unpublished.
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DR   MD5; d7ae8ca5aa85afed95f9b83e766e4aeb.
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CC   Contact: Wing RA
CC   Clemson University Genomics Institute
CC   Clemson University
CC   100 Jordan Hall, Clemson, SC 29634, USA
CC   Tel: 864 656 7288
CC   Fax: 864 656 4293
CC   Email: rwing@clemson.edu
CC   Seq primer: TAATACGACTCACTATAGGG
CC   Class: BAC ends
CC   High quality sequence stop: 185.
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FH   Key             Location/Qualifiers
FH
FT   source          1..666
FT                   /organism="Oryza sativa"
FT                   /lab_host="E. coli DH10B"
FT                   /strain="Japonica"
FT                   /cultivar="Nipponbare"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="CUGI Rice BAC Library"
FT                   /clone="nbxb0044F02f"
FT                   /tissue_type="Leaf"
FT                   /note="Vector: pBeloBAC11; Site_1: HindIII; Site_2:
FT                   HindIII; Rice is one of two most popular grains in the
FT                   world. Half of the world  population especially those
FT                   inhabiting highly populated areas of the  humid tropics and
FT                   subtropics, rely on rice as their primary source of
FT                   carbohydrate. Monocotyledonous rice is a diploid plant
FT                   (2n=24) with a  haploid genome equivalent of 431 Mbp
FT                   (Arumuganathan and Earle, 1991).  The relatively small
FT                   genome of rice, three times larger than that of
FT                   Arabidopsis, makes it suitable for genomic studies. In
FT                   order to  facilitate positional cloning, physical mapping
FT                   and genome sequencing  of rice, we have constructed a BAC
FT                   library from Oryza sativa, Nipponbare  variety. The library
FT                   contains 36,864 clones with an average insert size of 128.5
FT                   Kb providing 10.9 haploid genome equivalents. The deep 
FT                   coverage allows the isolation a particular sequence with a
FT                   probability  of 99.9 %. Two high density filters, each
FT                   containing 18,432 clones  (doubly spotted), represent the
FT                   whole library for colony screening."
FT                   /db_xref="taxon:4530"
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SQ   Sequence 666 BP; 140 A; 195 C; 64 G; 267 T; 0 other;
     catggatgat gcttactgtt gtatatcctg agattcatgt ttgaacaaag tttattgtga        60
     tcatttaagt atgagggtat tgtgtttatt tttttatctt tctacaggtt ctgatgatgc       120
     catgcagata gtgaatgcat tactggaagt tacttctcat tcagaatttg acatttcctc       180
     catgacattt aatttctggc atcatcttat gcgtaacctg accgataggt accactttac       240
     taaatgataa cttgtttttc acaagaaaca acccattcaa tgcttacaca tcttacgggt       300
     tcgttcgctt catatgaatt tcattcctca ctcatccttc ttgtaactca cctactactt       360
     cttcttcccc ctctctcttt tcccctctca cttctctcca ccttttacat tctctctctt       420
     tctactccac cctctcatct acactcacac tactcttttg tatccacttt ttcacccctc       480
     attcctcttc tctctccatt cactttctct cctcttaatt tatcttcctt ctcaccctta       540
     gcttccttta cacctccctc atacgtctcc ttctcttctt gaagaaaata tctcactatc       600
     ttacttctcc tacctaatcc cactcttatc ctatctgcag tcttattctc cgtgtcttca       660
     ctctcc                                                                  666
//