ID   AK190897; SV 1; linear; mRNA; HTC; MUS; 271 BP.
XX
AC   AK190897;
XX
DT   01-DEC-2004 (Rel. 82, Created)
DT   20-SEP-2008 (Rel. 97, Last updated, Version 2)
XX
DE   Mus musculus cDNA, clone:Y1G0104C22, strand:plus,
DE   reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000013262, based on BLAT
DE   search.
XX
KW   ASSETS; HTC.
XX
OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
XX
RN   [1]
RP   1-271
RA   Arakawa T., Carninci P., Fukuda S., Harbers M., Hayatsu N., Hori F.,
RA   Imotani K., Kawai J., Kondo S., Murata M., Nakamura M., Nomura K., Ohno M.,
RA   Sasaki D., Shiraki T., Waki K., Watahiki A., Hayashizaki Y.;
RT   ;
RL   Submitted (15-SEP-2004) to the INSDC.
RL   Contact:Yoshihide Hayashizaki The Institute of Physical and Chemical
RL   Research (RIKEN), Omics Science Center, RIKEN Yokohama Institute; 1-7-22
RL   Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan URL   
RL   :http://www.osc.riken.jp/
XX
RN   [2]
RX   DOI; 10.1038/nmeth719.
RX   PUBMED; 15782199.
RA   Watahiki A., Waki K., Hayatsu N., Shiraki T., Kondo S., Nakamura M.,
RA   Sasaki D., Arakawa T., Kawai J., Harbers M., Hayashizaki Y., Carninci P.;
RT   "Libraries enriched for alternatively spliced exons reveal splicing
RT   patterns in melanocytes and melanomas";
RL   Nat. Methods 1(3):233-239(2004).
XX
DR   MD5; b836a920e5bbcd54662962eac6f417ba.
XX
CC   Alternative Splicing Libraries (ASLs) are prepared by:
CC   Preparating of single-stranded DNA using a RNA template
CC   from full length cDNA libraries, hybridizating of
CC   single-stranded DNAs, removing of remaining single-stranded
CC   DNA, digesting of regions comprising double-stranded DNA
CC   by a set of 4 bp-cutters, capturing of DNA hybrids with loop
CC   structures (alternative spliced exon), ligating of Y-shaped
CC   primers to isolated DNA hybrids with loop structures, PCR
CC   amplification of ligation products and their cloning into
CC   pFLCI vector. (Reference)
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..271
FT                   /organism="Mus musculus"
FT                   /mol_type="mRNA"
FT                   /clone_lib="Alternative Splicing Library L1"
FT                   /clone="Y1G0104C22"
FT                   /cell_line="mixture of B16-F10Y and melan-c"
FT                   /cell_type="mixture of melanoma cell and melanocyte cell"
FT                   /note="strand:plus, reference:ENSEMBL:Mouse-Transcript-
FT                   ENST:ENSMUST00000013262, based on BLAT search"
FT                   /db_xref="taxon:10090"
XX
SQ   Sequence 271 BP; 69 A; 82 C; 71 G; 49 T; 0 other;
     cgcctctgcc aaaacaccag agagaccgag ctggaagttc aatttgtgaa gacctcaaga        60
     acagagacgg tgacttcgac tccaaagctt caagattgtg gcctgctggg gctattatcc       120
     tccaggagga cctgtccatc aaagccctgt gcccacgagt cttggctcca aaggaaagtg       180
     aggagcccag gaaaatgagg agtcctcctg gagagaaccc cagccctcag ggtgagcctc       240
     ccagcccaga gtcctctcga cgcctcttcc g                                      271
//