{"EMPIAR-14064":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of E. coli RNA polymerase on T7A1 promoter DNA with ATP, UTP, CTP - dataset 1","directory":"data/20230921Krios2_AM27-3/tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1440,"frames_per_image":35,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"data/20230921Krios2_AM27-3/tiffs/AM27-3_*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The micrographs contain unaligned raw frames. Data was collected on a 300 kV Titan Krios (ThermoFisher Scientific) equipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan) in counting mode (pixel size 0.86 A/px). Images were recorded using SerialEM over a nominal defocus range of -0.8 μm to -2.2 μm and 20 eV energy filter slit width. Collection was performed with an electron dose of 25 electrons/px/s (at the camera) in dose-fractionation mode of 0.04 seconds over a 1.4 second exposure (35 frames) to give a total dose of 47 electrons/A^2.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of E. coli RNA polymerase on T7A1 promoter DNA with ATP, UTP, CTP - dataset 2","directory":"data/20231018Krios2_AM27-1_AM27-2/tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":13806,"frames_per_image":35,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.43,"pixel_height":0.43,"micrographs_file_pattern":"data/20231018Krios2_AM27-1_AM27-2/tiffs/AM27-*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The micrographs contain unaligned raw frames. Data was collected on a 300 kV Titan Krios (ThermoFisher Scientific) equipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan) in super-resolution mode (nominal pixel size of 0.43 A/px). Images were recorded using SerialEM over a nominal defocus range of -0.8 μm to -2.2 μm and 20 eV energy filter slit width. Collection was performed with an electron dose of 25 electrons/px/s (at the camera) in dose-fractionation mode of 0.04 seconds over a 1.4 second exposure (35 frames) to give a total dose of 47 electrons/A^2.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of E. coli RNA polymerase on T7A1 promoter DNA with ATP, UTP, CTP - dataset 3","directory":"data/20231211Krios2_AM26-3/tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5256,"frames_per_image":35,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.43,"pixel_height":0.43,"micrographs_file_pattern":"data/20231211Krios2_AM26-3/tiffs/AM26-3_*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The micrographs contain unaligned raw frames. Data was collected on a 300 kV Titan Krios (ThermoFisher Scientific) equipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan) in super-resolution mode (nominal pixel size of 0.43 A/px). Images were recorded using SerialEM over a nominal defocus range of -0.8 μm to -2.2 μm and 20 eV energy filter slit width. Collection was performed with an electron dose of 25 electrons/px/s (at the camera) in dose-fractionation mode of 0.04 seconds over a 1.4 second exposure (35 frames) to give a total dose of 47 electrons/A^2.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of E. coli RNA polymerase on T7A1 promoter DNA with ATP, UTP, CTP - dataset 4","directory":"data/20231213Krios2_AM26-4/tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":6372,"frames_per_image":35,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.43,"pixel_height":0.43,"micrographs_file_pattern":"data/20231213Krios2_AM26-4/tiffs/AM26-4_*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The micrographs contain unaligned raw frames. Data was collected on a 300 kV Titan Krios (ThermoFisher Scientific) equipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan) in super-resolution mode (nominal pixel size of 0.43 A/px). Images were recorded using SerialEM over a nominal defocus range of -0.8 μm to -2.2 μm and 20 eV energy filter slit width. Collection was performed with an electron dose of 25 electrons/px/s (at the camera) in dose-fractionation mode of 0.04 seconds over a 1.4 second exposure (35 frames) to give a total dose of 47 electrons/A^2.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R35GM118130","country":"United States"}],"version_history":[],"title":"Cryo-EM of E. coli RNA polymerase initial transcribing complexes on T7A1 promoter","principal_investigator":[{"author_orcid":"0000-0002-8241-3153","middle_name":"A","organization":"The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Seth","last_name":"Darst","email":"darst [at] rockefeller.edu","country":"United States","entry":"EMPIAR-14064"}],"status":"REL","deposition_date":"2026-09-16","release_date":"2026-10-29","obsolete_date":null,"update_date":"2026-10-29","corresponding_author":{"author":{"author_orcid":"0000-0002-8241-3153","middle_name":"A","organization":"The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","first_name":"Seth","last_name":"Darst","country":"United States"}},"authors":[{"author":{"name":"Mueller AU","author_orcid":"0000-0003-3002-3695"}},{"author":{"name":"Darst SA","author_orcid":"0000-0002-8241-3153"}}],"cross_references":["EMD-73123","EMD-73125","EMD-73126","EMD-73124","EMD-73128","EMD-73130","EMD-73122","EMD-73127","EMD-73131"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Mueller AU","author_orcid":"0000-0003-3002-3695"},{"name":"Darst SA","author_orcid":"0000-0002-8241-3153"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis for multi-subunit DNA-dependent RNA polymerase catalytic activity","volume":"86","country":"","first_page":"1881","last_page":"189500000.0","year":"2026","language":"English","doi":"10.1016/j.molcel.2026.03.033","pubmedid":"42066755","details":"The micrographs contain unaligned raw frames grouped into 4 datasets. Data was collected on a 300 kV Titan Krios (ThermoFisher Scientific) equipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan) in counting mode (pixel size 0.86 A/px) or super-resolution mode (nominal pixel size of 0.43 A/px). Images were recorded using SerialEM over a nominal defocus range of -0.8 μm to -2.2 μm and 20 eV energy filter slit width. Collection was performed with an electron dose of 25 electrons/px/s (at the camera) in dose-fractionation mode of 0.04 seconds over a 1.4 second exposure (35 frames) to give a total dose of 47 electrons/A^2.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Molecular cell","journal_abbreviation":"Mol Cell","issue":"10","preprint":false}],"dataset_size":"15.2 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9ymv","9ymx","9ymy","9ymw","9ymu","9yn0"],"entry_doi":"10.6019/EMPIAR-14064"}}