{"EMPIAR-14061":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame EER movies of the Mycobacterium tuberculosis ClpC1P1P2 protease complex bound to the activator Bz-Leu-Leu","directory":"data","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":10943,"frames_per_image":666,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.5248,"pixel_height":0.5248,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw, unaligned EER movies (not gain-corrected) recorded with EPU on a Talos Arctica (Thermo Fisher Scientific) operated at 200 kV (FEG, Cs 2.7 mm) and equipped with a Falcon 4i detector. Nominal magnification 190,000x; pixel size 0.5248 A/px (0.525 A/px in EMDB) for the 4096 x 4096 images, i.e. without EER upsampling. Each movie contains 666 EER frames; total fluence approximately 40 e-/A^2 (38.6-40.1 e-/A^2 according to the EER headers). Nominal defocus range -0.8 to -2.4 um. Folder layout: data/movies/batch_01-03 contains the 10,943 EER movies; data/epu_metadata/batch_01-03 contains the 10,942 corresponding EPU XML metadata files (one movie has no XML file); data/gain_reference/20240318_163908_EER_GainReference.gain is the gain reference to be applied to the EER movies. These movies were processed in cryoSPARC 4.6 to obtain EMD-52764, EMD-52765, EMD-52766 and EMD-52840 (PDB 9IF4). Acquisition values were read from the raw data headers and EPU metadata; Table 1 of the associated publication lists 120,000x magnification, 46 e-/A^2 and a defocus range of -0.8 to -2.2 um.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Fundacao para a Ciencia e a Tecnologia","code":"2024.13543.PEX","country":"Portugal"},{"funding_body":"Fundacao para a Ciencia e a Tecnologia","code":"2024.04906.RESTART","country":"Portugal"},{"funding_body":"Fundacao para a Ciencia e a Tecnologia","code":"2001.00654.CEECIND","country":"Portugal"},{"funding_body":"European Union (EU)","code":"101004806","country":"European Union"},{"funding_body":"Instruct-ERIC","code":"PID 26164","country":"United Kingdom"},{"funding_body":"Instruct-ERIC","code":"PID 30641","country":"United Kingdom"},{"funding_body":"ISIDORe","code":"101046133","country":"European Union"},{"funding_body":"Fundacao para a Ciencia e a Tecnologia","code":"UID/04612/2025","country":"Portugal"},{"funding_body":"Fundacao para a Ciencia e a Tecnologia","code":"UID/PRR/4612/2025","country":"Portugal"},{"funding_body":"Fundacao para a Ciencia e a Tecnologia","code":"LA/P/0087/2020","country":"Portugal"}],"version_history":[],"title":"Cryo-EM movies of the Mycobacterium tuberculosis ClpC1P1P2 protease complex bound to the activator Bz-Leu-Leu","principal_investigator":[{"author_orcid":"0000-0001-7677-4086","middle_name":null,"organization":"i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto","street":"Rua Alfredo Allen, 208","town_or_city":"Porto","state_or_province":null,"post_or_zip":"4200-135","telephone":null,"fax":null,"first_name":"Hugo","last_name":"Fraga","email":"hugo.fraga [at] i3s.up.pt","country":"Portugal","entry":"EMPIAR-14061"}],"status":"REL","deposition_date":"2026-10-15","release_date":"2026-11-08","obsolete_date":null,"update_date":"2026-11-08","corresponding_author":{"author":{"author_orcid":"0000-0003-1622-9443","middle_name":"Aleksandrovych","organization":"Instituto de Tecnologia Química e Biológica António Xavier (ITQB NOVA), Universidade Nova de Lisboa","street":"Av. da República","town_or_city":"Oeiras","state_or_province":null,"post_or_zip":"2780-157","first_name":"Dmitry","last_name":"Semchonok","country":"Portugal"}},"authors":[{"author":{"name":"Weinhäupl K","author_orcid":"0000-0002-9552-9876"}},{"author":{"name":"Semchonok DA","author_orcid":"0000-0003-1622-9443"}},{"author":{"name":"Gragera M","author_orcid":"0000-0002-3097-1743"}},{"author":{"name":"Arranz R","author_orcid":"0000-0001-5321-0915"}},{"author":{"name":"Bueno-Carrasco MT","author_orcid":"0000-0003-1586-2589"}},{"author":{"name":"Fraga H","author_orcid":"0000-0001-7677-4086"}}],"cross_references":["EMD-52840","EMD-52765","EMD-52764","EMD-52766"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Weinhäupl K","author_orcid":"0000-0002-9552-9876"},{"name":"Akopian T","author_orcid":null},{"name":"Semchonok DA","author_orcid":"0000-0003-1622-9443"},{"name":"Krandor O","author_orcid":null},{"name":"Arranz R","author_orcid":"0000-0001-5321-0915"},{"name":"Bueno-Carrasco MT","author_orcid":"0000-0003-1586-2589"},{"name":"Gragera M","author_orcid":"0000-0002-3097-1743"},{"name":"Chevreuil M","author_orcid":null},{"name":"Raynal B","author_orcid":"0000-0001-5634-0408"},{"name":"Zinga S","author_orcid":"0000-0002-4633-8299"},{"name":"Liu Y","author_orcid":null},{"name":"Lai J","author_orcid":"0009-0003-4566-1961"},{"name":"Wu W","author_orcid":null},{"name":"Bachovchin W","author_orcid":"0000-0002-9926-7244"},{"name":"Gauto D","author_orcid":"0000-0003-3757-2830"},{"name":"Goldberg A","author_orcid":"0000-0002-8319-5856"},{"name":"Rubin E","author_orcid":"0000-0001-5120-962X"},{"name":"Fraga H","author_orcid":"0000-0001-7677-4086"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Activation mechanism and structural assembly of the Mycobacterium tuberculosis ClpP1P2 protease and its associated ATPases","volume":"45","country":"United States","first_page":null,"last_page":null,"year":"2026","language":"English","doi":"10.1016/j.celrep.2026.117400","pubmedid":"42213777","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Cell Reports","journal_abbreviation":"Cell Rep","issue":"6","preprint":false}],"dataset_size":"2.5 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9if4"],"entry_doi":"10.6019/EMPIAR-14061"}}