{"EMPIAR-13971":{"imagesets":[{"segmentations":[],"name":"Unaligned raw movies","directory":"data/tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":34503,"frames_per_image":35,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.847,"pixel_height":0.847,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Grids were imaged using a 300 kV Titan Krios (ThermoFisher Scientific) equipped with\na K3 camera (Gatan). Images were recorded using SerialEM with a pixel size of 0.847 Å/px over\na nominal defocus range of −0.6 to −2.0 μm and 20 eV energy filter slit width. A total of 34,503\n(AAP-SO2 minus) gain-normalized movies were recorded in counting\nmode (image dimensions of 4092 × 5760 px) with 27.5 e−/px/s (at the camera) in dose fractionation\nmode of 0.04 s over a 1.4 s exposure (35 frames) to give a total dose of 50.7 e−/Å2.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R01 GM038660","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R35 GM151879","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R35 GM118130","country":"United States"}],"version_history":[],"title":"Cryo-EM of Mtb RNAP elongation complex (substrate loading mimic)","principal_investigator":[{"author_orcid":"0000-0002-8241-3153","middle_name":"A.","organization":"The Rockefeller University","street":"1230 York Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Seth","last_name":"Darst","email":"darst [at] rockefeller.edu","country":"United States","entry":"EMPIAR-13971"}],"status":"REL","deposition_date":"2026-09-03","release_date":"2026-09-24","obsolete_date":null,"update_date":"2026-09-24","corresponding_author":{"author":{"author_orcid":"0000-0002-8241-3153","middle_name":"A.","organization":"The Rockefeller University","street":"1230 York Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","first_name":"Seth","last_name":"Darst","country":"United States"}},"authors":[{"author":{"name":"Dhingra Y.","author_orcid":"0000-0001-9060-8201"}},{"author":{"name":"Landick R.","author_orcid":"0000-0002-5042-0383"}},{"author":{"name":"Campbell E.A.","author_orcid":"0000-0002-1332-128X"}},{"author":{"name":"Darst S.A.","author_orcid":"0000-0002-8241-3153"}}],"cross_references":["EMD-75288","EMD-75286","EMD-75287"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Dhingra Y.","author_orcid":"0000-0001-9060-8201"},{"name":"Landick R.","author_orcid":"0000-0002-5042-0383"},{"name":"Campbell E.A.","author_orcid":"0000-0002-1332-128X"},{"name":"Darst S.A.","author_orcid":"0000-0002-8241-3153"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"RNA polymerase inhibitors reveal active-site motions essential for the nucleotide addition cycle","volume":"123","country":"United States","first_page":"e2609228123","last_page":"e2609228123","year":"2026","language":"English","doi":"10.1073/pnas.2609228123","pubmedid":"42378287","details":"Cryo-EM movies of Mycobacterium tuberculosis RNAP elongation complex (substrate loading mimic) collected on a 300 kV Titan Krios microscope equipped with a Gatan K3 direct electron detector, Cs corrector, and BioQuantum imaging filter. Movies were recorded using SerialEM with a pixel size of 0.847 Å/px over a nominal defocus range of −0.6 to −2.0 μm and 20 eV energy filter slit width. A total of 34,503 (AAP-SO2 minus) gain-normalized movies were recorded in counting mode (image dimensions of 4092 × 5760 px) with 27.5 e−/px/s (at the camera) in dose fractionation mode of 0.04 s over a 1.4 s exposure (35 frames) to give a total dose of 50.7 e−/Å2.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proceedings of the National Academy of Sciences","journal_abbreviation":"PNAS","issue":"27","preprint":false}],"dataset_size":"5.9 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["10mk","10mi","10mj"],"entry_doi":"10.6019/EMPIAR-13971"}}