{"EMPIAR-13968":{"imagesets":[{"segmentations":[],"name":"Unaligned raw movies","directory":"data/tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":28709,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.676,"pixel_height":0.676,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Grids were imaged using a 300 kV Titan Krios (ThermoFisher Scientific)\nequipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan).\nImages were recorded using SerialEM with a pixel size of 0.676 Å/px over a nominal defocus\nrange of −0.6 to −2.0 μm and 20 eV energy filter slit width. Gain-normalized movies were recorded in counting mode (K3 camera; image dimensions of 4092 × 5760 px) with 20 e−/px/s (at the camera) in dose-fractionation mode of 0.03 s over a 1.2 s exposure (40 frames) to give a total dose of 52.5 e−/Å2.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R01 GM038660","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R35 GM151879","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R35 GM118130","country":"United States"}],"version_history":[],"title":"Cryo-EM of Eco RNAP his-elemental paused elongation complex with CBR9379","principal_investigator":[{"author_orcid":"0000-0002-8241-3153","middle_name":"A.","organization":"The Rockefeller University","street":"1230 York Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Seth","last_name":"Darst","email":"darst [at] rockefeller.edu","country":"United States","entry":"EMPIAR-13968"}],"status":"REL","deposition_date":"2026-09-01","release_date":"2026-09-24","obsolete_date":null,"update_date":"2026-09-24","corresponding_author":{"author":{"author_orcid":"0000-0002-8241-3153","middle_name":"A.","organization":"The Rockefeller University","street":"1230 York Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","first_name":"Seth","last_name":"Darst","country":"United States"}},"authors":[{"author":{"name":"Dhingra Y.","author_orcid":"0000-0001-9060-8201"}},{"author":{"name":"Landick R.","author_orcid":"0000-0002-5042-0383"}},{"author":{"name":"Campbell E.A.","author_orcid":"0000-0002-1332-128X"}},{"author":{"name":"Darst S.A.","author_orcid":"0000-0002-8241-3153"}}],"cross_references":["EMD-75285","EMD-75284"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Dhingra Y.","author_orcid":"0000-0001-9060-8201"},{"name":"Landick R.","author_orcid":"0000-0002-5042-0383"},{"name":"Campbell E.A.","author_orcid":"0000-0002-1332-128X"},{"name":"Darst S.A.","author_orcid":"0000-0002-8241-3153"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"RNA polymerase inhibitors reveal active-site motions essential for the nucleotide addition cycle","volume":"123","country":"United States","first_page":"e2609228123","last_page":"e2609228123","year":"2026","language":"English","doi":"10.1073/pnas.2609228123","pubmedid":"42378287","details":"Cryo-EM movies of the Escherichia coli his-ePEC (elemental his pause elongation complex) with CBR9379 collected on a 300 kV Titan Krios microscope equipped with a Gatan K3 direct electron detector, Cs corrector, and BioQuantum imaging filter. Movies were recorded using SerialEM at a physical pixel size of 0.676 Å/pixel over a nominal defocus range of −0.6 to −2.0 μm with a 20 eV energy filter slit. Data were acquired in counting mode using dose-fractionation with 40 frames collected over a 1.2 s exposure at a total dose of 52.5 e⁻/Ų. The dataset comprises gain-normalized raw movies collected for single-particle cryo-EM structure determination of the E. coli his-ePEC.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proceedings of the National Academy of Sciences","journal_abbreviation":"PNAS","issue":"27","preprint":false}],"dataset_size":"4.5 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["10mg","10mf"],"entry_doi":"10.6019/EMPIAR-13968"}}