{"EMPIAR-13965":{"imagesets":[{"segmentations":[],"name":"unaligned movies of a MEK1-ERK2 complex with ADP.AlF","directory":"data/CM02_2_HAFnotilt","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":88517,"frames_per_image":58,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.46,"pixel_height":0.46,"micrographs_file_pattern":"data/CM02_2_HAFnotilt/raw_movies/raw_0/FoilHole_*.tiff","picked_particles_file_pattern":"data/particles/J2308_particles.tar.gz","picked_particles_directory":"data/particles/J2308_particles.tar.gz","details":"Dataset of 88 517 movies collected at 270kx magnification on a Titan Krios with Falcon IV camera. Sample is the stabilised complex of two human protein kinases (MEK1 and ERK2) with the transition-state analog ADP.AlF. Deposited are the raw movie files in TIFF format and XML meta data for exposure group information. Processing resulted in three maps in separate states of the complex, for which the total particle coordinates for final reconstructions of all states are deposited in a .star file along with the exported cryoSPARC job.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single-particle cryo-EM movies of the MAP2K MEK1 in complex with its substrate MAPK ERK2","principal_investigator":[{"author_orcid":"0000-0003-0465-3351","middle_name":"W","organization":"EMBL Grenoble","street":"71 Avenue des Martyrs","town_or_city":"Grenoble","state_or_province":"Isere","post_or_zip":"38000","telephone":null,"fax":null,"first_name":"Matthew","last_name":"Bowler","email":"mbowler [at] embl.fr","country":"France","entry":"EMPIAR-13965"}],"status":"REL","deposition_date":"2026-05-05","release_date":"2026-09-29","obsolete_date":null,"update_date":"2026-09-29","corresponding_author":{"author":{"author_orcid":"0000-0003-0465-3351","middle_name":"W","organization":"EMBL Grenoble","street":"71 Avenue des Martyrs","town_or_city":"Grenoble","state_or_province":"Isere","post_or_zip":"38000","first_name":"Matthew","last_name":"Bowler","country":"France"}},"authors":[{"author":{"name":"von Velsen J","author_orcid":"0000-0002-1254-7842"}},{"author":{"name":"Juyoux P","author_orcid":"0000-0003-3153-1376"}},{"author":{"name":"Bowler MW","author_orcid":"0000-0003-0465-3351"}}],"cross_references":["EMD-56420","EMD-56419","EMD-56418"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"von Velsen J","author_orcid":"0000-0002-1254-7842"},{"name":"Juyoux P","author_orcid":"0000-0003-3153-1376"},{"name":"Piasentin N","author_orcid":"0000-0003-2760-4640"},{"name":"Fisher H","author_orcid":"0000-0003-0093-0921"},{"name":"Lapouge K","author_orcid":"0000-0003-0620-9553"},{"name":"Vadas O","author_orcid":"0000-0003-3511-6479"},{"name":"Gervasio FL","author_orcid":"0000-0003-4831-5039"},{"name":"Bowler MW","author_orcid":"0000-0003-0465-3351"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Molecular basis of mitogen-activated protein kinase ERK2 activation by its upstream kinase MEK1","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.64898/2026.01.19.700303","pubmedid":"41648251","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"biorxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"7.9 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9tyi","9tyh","9tyg"],"entry_doi":"10.6019/EMPIAR-13965"}}