{"EMPIAR-13850":{"imagesets":[{"segmentations":[],"name":"Cryo-EM structure of TrhO from B. subtilis complexed with tRNA Ala","directory":"data","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":10314,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.9013,"pixel_height":0.9013,"micrographs_file_pattern":"data/GridSquare_*/Data/*.eer","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Videos were collected using EPU (version 2.14.0; Thermo Fisher Scientific) in counting mode with a total dose of 60 e− per Å2 for a 7.15-s exposure. A total of 10,314 videos were recorded in EER format at a nominal magnification of ×130,000, corresponding to a physical pixel size of 0.9013 Å, with a defocus range from −0.6 to −2.1 μm.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"National Research Foundation (NRF, Korea)","code":"RS-2024-00411137","country":"Korea, South"},{"funding_body":"National Research Foundation (NRF, Korea)","code":"RS-2024-00440614","country":"Korea, South"},{"funding_body":"National Research Foundation (NRF, Korea)","code":"RS-2025-23323757","country":"Korea, South"}],"version_history":[],"title":"Cryo-EM structure of TrhO from B. subtilis complexed with tRNA Ala","principal_investigator":[{"author_orcid":"0000-0001-5213-9299","middle_name":null,"organization":"Department of Chemistry, Gwangju Institute of Science and Technology","street":"123, Cheomdangwagi-ro, Buk-gu","town_or_city":"Jeonnam-Gwangju","state_or_province":null,"post_or_zip":"61005","telephone":null,"fax":null,"first_name":"Jungwook","last_name":"Kim","email":"jwkim [at] gist.ac.kr","country":"Korea, South","entry":"EMPIAR-13850"}],"status":"REL","deposition_date":"2026-07-27","release_date":"2026-08-13","obsolete_date":null,"update_date":"2026-08-13","corresponding_author":{"author":{"author_orcid":"0000-0001-6785-616X","middle_name":null,"organization":"Department of Chemistry, Gwangju Institute of Science and Technology","street":"123, Cheomdangwagi-ro, Buk-gu","town_or_city":"Jeonnam-Gwangju","state_or_province":null,"post_or_zip":"61005","first_name":"Gyuhyeok","last_name":"Cho","country":"Korea, South"}},"authors":[{"author":{"name":"Shin K","author_orcid":"0009-0009-4788-0539"}},{"author":{"name":"Kim J","author_orcid":"0000-0001-5213-9299"}}],"cross_references":["EMD-61148"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Shin K","author_orcid":"0009-0009-4788-0539"},{"name":"Han DB","author_orcid":"0009-0007-3948-4873"},{"name":"Kim HW","author_orcid":"0000-0002-2472-2046"},{"name":"Kim J","author_orcid":"0000-0001-5213-9299"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Unconventional monooxygenation by the O<sub>2</sub>-dependent tRNA wobble uridine hydroxylase TrhO","volume":"22","country":"","first_page":"886","last_page":"894","year":"2026","language":"English","doi":"10.1038/s41589-025-02129-2","pubmedid":"41554887","details":"Videos were collected using EPU (version 2.14.0; Thermo Fisher Scientific) in counting mode with a total dose of 60 e− per Å2 for a 7.15-s exposure. A total of 10,314 videos were recorded in EER format at a nominal magnification of ×130,000, corresponding to a physical pixel size of 0.9013 Å, with a defocus range from −0.6 to −2.1 μm.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature chemical biology","journal_abbreviation":"Nat Chem Biol","issue":"6","preprint":false}],"dataset_size":"10.8 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9j5o"],"entry_doi":"10.6019/EMPIAR-13850"}}