{"EMPIAR-13782":{"imagesets":[{"segmentations":[],"name":"Motion corrected, SARS-CoV-2 spike protein in complex with human neutralizing antibody WRAIR-2008","directory":"data/micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":10567,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.873,"pixel_height":0.873,"micrographs_file_pattern":"data/micrographs/*.mrc","picked_particles_file_pattern":"data/refined_particles.star","picked_particles_directory":"","details":"Motion corrected, SARS-CoV-2 spike protein in complex with human neutralizing antibody WRAIR-2008 collected using SerialEM on a K3 camera in counting, non-CDS mode on a JEOL CryoARM 200 operated at 200kV (Cs=2.7) in EFTEM mode with a 20eV slit. The nominal dose per movie was ~60 e/Å^2 and motion-correction was performed using CryoSPARC v4.4.0.\n\nPicked particle stack is not included, but alignment metadata for final global reconstruction (EMDB: 48284 PDB: 9mi3 (global)) is included in refined_particles.star. Instructions for CryoSPARC or RELION import and reextraction are provided in README.txt.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Static Masks used for Focused Local Refinement","directory":"data/static_masks","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.023,"pixel_height":1.023,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Static Masks used for Focused Local Refinement EMDB: 47928 PDB: 9ecz (focused refinement)","image_width":"512","image_height":"512"}],"workflow_file":null,"grant_references":[{"funding_body":"Department of Defense (DOD, United States)","code":"W81XWH-18-2-0040","country":"United States"}],"version_history":[],"title":"Cryo-EM structure of SARS-CoV-2 spike protein in complex with human neutralizing antibody WRAIR-2008","principal_investigator":[{"author_orcid":"0000-0002-6808-7232","middle_name":"Gordon","organization":"Henry M Jackson Foundation","street":"503 Robert Grant Ave","town_or_city":"Silver Spring","state_or_province":"MD","post_or_zip":"20910","telephone":"3013197528","fax":null,"first_name":"Michael","last_name":"Joyce","email":"gjoyce [at] global-id.org","country":"United States","entry":"EMPIAR-13782"}],"status":"REL","deposition_date":"2025-08-14","release_date":"2026-07-20","obsolete_date":null,"update_date":"2026-07-20","corresponding_author":{"author":{"author_orcid":"0000-0002-6772-3997","middle_name":null,"organization":"Henry M Jackson Foundation","street":"503 Robert Grant Ave","town_or_city":"Silver Spring","state_or_province":"MD","post_or_zip":"20910","first_name":"Paul","last_name":"Thomas","country":"United States"}},"authors":[{"author":{"name":"Jensen JLJ","author_orcid":"0000-0002-8818-7513"}},{"author":{"name":"Thomas PVT","author_orcid":"0000-0002-6772-3997"}},{"author":{"name":"Joyce MGJ","author_orcid":"0000-0002-6808-7232"}}],"cross_references":["EMD-48284"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Jensen JLJ","author_orcid":"0000-0002-8818-7513"},{"name":"Thomas PVT","author_orcid":"0000-0002-6772-3997"},{"name":"Joyce MGJ","author_orcid":"0000-0002-6808-7232"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"First-generation N-terminal domain supersite public antibodies retain activity against Omicron-derived lineages and protect mice against Omicron BA.5 challenge","volume":null,"country":"United States","first_page":null,"last_page":null,"year":null,"language":"English","doi":"10.1128/mbio.01036-25","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"mBio","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"928.7 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9mi3"],"entry_doi":"10.6019/EMPIAR-13782"}}