{"EMPIAR-13658":{"imagesets":[{"segmentations":[],"name":"unaligned multi-frame micrographs of ThermoCas9 incubated with methylated DNA target with NNNNCCA PAM","directory":"data/CC-PAM-methylatedDNA","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":6603,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.415,"pixel_height":0.415,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This dataset was collected on K3 camera at 105k magnification using EPU, with a super-resolution pixel size of 0.415 and a  total dose of 60 spreading over 60 frames. Gain file is also provided. 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Gain orientation in Relion: no rotation, Flip upside-down;  in cryosparc: no rotation, no flip.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"unaligned multi-frame micrographs of ThermoCas9 incubated with non-methylated DNA target with NNNNCGA PAM","directory":"data/CG-PAM-nonmethylatedDNA","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":12596,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.415,"pixel_height":0.415,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"S10RR025080","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"U24GM116788","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"S10RR024564","country":"United States"},{"funding_body":"Department of Energy (DOE, United States)","code":"KP160711","country":"United States"}],"version_history":[],"title":"Cryo-EM Structures of Methylation-dependent Cas9","principal_investigator":[{"author_orcid":"0000-0003-2046-9861","middle_name":null,"organization":"Department of Structural Biology, Van Andel Institute","street":null,"town_or_city":"Grand Rapids","state_or_province":"Michigan","post_or_zip":"49503","telephone":null,"fax":null,"first_name":"Hong","last_name":"Li","email":"hong.li [at] vai.org","country":"United States","entry":"EMPIAR-13658"}],"status":"REL","deposition_date":"2026-06-11","release_date":"2026-06-28","obsolete_date":null,"update_date":"2026-06-28","corresponding_author":{"author":{"author_orcid":"0000-0003-2046-9861","middle_name":null,"organization":"Department of Structural Biology, Van Andel Institute","street":null,"town_or_city":"Grand Rapids","state_or_province":"Michigan","post_or_zip":"49503","first_name":"Hong","last_name":"Li","country":"United States"}},"authors":[{"author":{"name":"Shu Y","author_orcid":"0009-0000-8986-0725"}},{"author":{"name":"Zhao Y","author_orcid":"0000-0003-0485-2780"}},{"author":{"name":"Li H","author_orcid":"0000-0003-2046-9861"}}],"cross_references":["EMD-44859","EMD-43770","EMD-43769","EMD-43771","EMD-43772"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Roth MO","author_orcid":null},{"name":"Shu Y","author_orcid":"0009-0000-8986-0725"},{"name":"Zhao Y","author_orcid":"0000-0003-0485-2780"},{"name":"Trasanidou D","author_orcid":null},{"name":"Hoffman RD","author_orcid":null},{"name":"Südfeld C","author_orcid":null},{"name":"Bouzetos E","author_orcid":null},{"name":"Trasanidis N","author_orcid":null},{"name":"Zawrotny M","author_orcid":null},{"name":"Gelasco MK","author_orcid":null},{"name":"Medina ML","author_orcid":null},{"name":"Das A","author_orcid":null},{"name":"Rai J","author_orcid":null},{"name":"Goswami HN","author_orcid":null},{"name":"Wang B","author_orcid":null},{"name":"van der Oost J","author_orcid":null},{"name":"Li H","author_orcid":"0000-0003-2046-9861"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Molecular basis for methylation-sensitive editing by Cas9","volume":"653","country":"","first_page":"1229","last_page":"1239","year":"2026","language":"English","doi":"10.1038/s41586-026-10384-z","pubmedid":"41986708","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":"8116","preprint":false}],"dataset_size":"11.1 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9bs6","9ar5","9ar4","9ar6","9ar7"],"entry_doi":"10.6019/EMPIAR-13658"}}