{"EMPIAR-13642":{"imagesets":[{"segmentations":[],"name":"unaligned multi-frame micrographs of yeast hypomethylated 80S ribosome treated with hygromycin B","directory":"data/HygromycinB-treated","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":9063,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.365,"pixel_height":0.365,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This dataset was collected on Falcon 4 camera in EER mode using Leginon, with a super-resolution pixel size of 0.365 and a  total dose of 55.20 e–/Å2. The authors used EER fractionation of 32 and dose per frame of 0.82 with a binning factor 2 for motion correction.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"unaligned multi-frame micrographs of yeast nutrient limiting hypomethylated 80S ribosome","directory":"data/Nutrient-limiting","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":10148,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.53,"pixel_height":0.53,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This dataset was collected on K3 camera at 81k magnification using EPU, with a super-resolution pixel size of 0.53 and a  total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down;  in cryosparc: no rotation, no flip.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"unaligned multi-frame micrographs of yeast hypomethylated polysomes at 81K magnification","directory":"data/Polysome-81K","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7659,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.53,"pixel_height":0.53,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This dataset was collected on K3 camera at 81k magnification using EPU, with a super-resolution pixel size of 0.53 and a  total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down;  in cryosparc: no rotation, no flip.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"unaligned multi-frame micrographs of yeast hypomethylated polysomes at 105K magnification","directory":"data/Polysome-105K","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5585,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.415,"pixel_height":0.415,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This dataset was collected on K3 camera at 81k magnification using EPU, with a super-resolution pixel size of 0.415 and a  total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down;  in cryosparc: no rotation, no flip.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"unaligned multi-frame micrographs of yeast hypomethylated 80S ribosome bound with TSV-IRES, eEF2, sodarin","directory":"data/TSV-bound","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7369,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.53,"pixel_height":0.53,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This dataset was collected on K3 camera at 81k magnification using serialEM, with a super-resolution pixel size of 0.53 and a  total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM single particle micrographs of hypomethylated 80S ribosomes","principal_investigator":[{"author_orcid":"0000-0003-2046-9861","middle_name":null,"organization":"Department of Structural Biology, Van Andel Institute","street":null,"town_or_city":"Grand Rapids","state_or_province":"Michigan","post_or_zip":"49503","telephone":null,"fax":null,"first_name":"Hong","last_name":"Li","email":"hong.li [at] vai.org","country":"United States","entry":"EMPIAR-13642"}],"status":"REL","deposition_date":"2024-03-01","release_date":"2026-06-25","obsolete_date":null,"update_date":"2026-06-25","corresponding_author":{"author":{"author_orcid":"0000-0003-2046-9861","middle_name":null,"organization":"Department of Structural Biology, Van Andel Institute","street":null,"town_or_city":"Grand Rapids","state_or_province":"Michigan","post_or_zip":"49503","first_name":"Hong","last_name":"Li","country":"United States"}},"authors":[{"author":{"name":"Zhao Y","author_orcid":"0000-0003-0485-2780"}},{"author":{"name":"Li H","author_orcid":"0000-0003-2046-9861"}}],"cross_references":["EMD-71745","EMD-41001","EMD-40990","EMD-40997","EMD-40998","EMD-40999","EMD-41000","EMD-40992","EMD-41002","EMD-40993"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Zhao Y","author_orcid":null},{"name":"Rai J","author_orcid":null},{"name":"Cohen LN","author_orcid":null},{"name":"Shu Y","author_orcid":null},{"name":"Xu C","author_orcid":null},{"name":"Goswami H","author_orcid":null},{"name":"Chen X","author_orcid":null},{"name":"Jin H","author_orcid":null},{"name":"Marchand V","author_orcid":null},{"name":"Motorin Y","author_orcid":null},{"name":"Ghalei H","author_orcid":null},{"name":"Li H","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"2'-O-Methylation maintains ribosome structural and translation integrity","volume":"86","country":"","first_page":"1495","last_page":"1510.e7","year":"2026","language":"English","doi":"10.1016/j.molcel.2026.03.008","pubmedid":"41928510","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Molecular cell","journal_abbreviation":"Mol Cell","issue":"8","preprint":false}],"dataset_size":"24.2 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9pn5","8t3e","8t2x","8t3a","8t3b","8t3c","8t3d","8t2z","8t3f","8t30"],"entry_doi":"10.6019/EMPIAR-13642"}}