{"EMPIAR-13634":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrograph movies of the co-transcriptional pre-60S intermediates -- Dataset 3","directory":"data/ds3","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":30768,"frames_per_image":50,"frame_range_min":1,"frame_range_max":50,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.7 to -1.7 µm, and a total dose of 50.0 e-/Å2 (50 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrograph movies of the co-transcriptional pre-60S intermediates -- Dataset 4","directory":"data/ds4","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":9708,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.545,"pixel_height":0.545,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 81,000× with a pixel size of 0.545 Å/pixel, a defocus range of -0.6 to -1.7 µm, and a total dose of 50.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrograph movies of the co-transcriptional pre-60S intermediates -- Dataset 5","directory":"data/ds5","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":10799,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.545,"pixel_height":0.545,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 81,000× with a pixel size of 0.545 Å/pixel, a defocus range of -0.6 to -1.7 µm, and a total dose of 50.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrograph movies of the co-transcriptional pre-60S intermediates -- Dataset 6","directory":"data/ds6","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":10632,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.545,"pixel_height":0.545,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 81,000× with a pixel size of 0.545 Å/pixel, a defocus range of -0.6 to -1.7 µm, and a total dose of 50.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Polished particle stacks of Pwp1-RNP and Nop12-RNP co-transcriptional pre-60S intermediates","directory":"data/particles","category":"picked particles - multiframe - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":2544054,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Particle sets used to reconstruct the pre-60S intermediates from all datasets. The particles were polished in cryoSPARC. For each reconstruction, the provided STAR file contains the patch-CTF estimated defocus and CTF corrections, as well as the final Euler angles, shifts, and micrograph coordinates.","image_width":"300","image_height":"300"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"5R01GM145950-03","country":"United States"},{"funding_body":"Chan Zuckerberg Initiative","code":"2023-332391","country":"United States"}],"version_history":[],"title":"Single particle cryo-EM dataset of yeast co-transcriptional pre-60S assembly intermediates Pwp1 RNP and Nop12 RNP","principal_investigator":[{"author_orcid":"0000-0002-9373-4737","middle_name":null,"organization":"Rockefeller University","street":"1230 York Ave","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Sebastian","last_name":"Klinge","email":"klinge [at] rockefeller.edu","country":"United States","entry":"EMPIAR-13634"}],"status":"REL","deposition_date":"2026-05-12","release_date":"2026-07-07","obsolete_date":null,"update_date":"2026-07-07","corresponding_author":{"author":{"author_orcid":"0000-0002-9373-4737","middle_name":null,"organization":"Rockefeller University","street":"1230 York Ave","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","first_name":"Sebastian","last_name":"Klinge","country":"United States"}},"authors":[{"author":{"name":"Piwowarczyk R","author_orcid":"0000-0001-6463-916X"}},{"author":{"name":"Klinge S","author_orcid":"0000-0002-9373-4737"}}],"cross_references":["EMD-75577","EMD-75580","EMD-75578","EMD-75579"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Piwowarczyk R","author_orcid":"0000-0001-6463-916X"},{"name":"Klinge S","author_orcid":"0000-0002-9373-4737"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Mechanism for the initiation of co-transcriptional pre-60 <i>S</i> assembly","volume":null,"country":"","first_page":null,"last_page":null,"year":"2026","language":null,"doi":"10.64898/2026.05.22.727207","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"32.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["10zy","11aa","10zz"],"entry_doi":"10.6019/EMPIAR-13634"}}