{"EMPIAR-13621":{"imagesets":[{"segmentations":[],"name":"Template picked particles from the 57s turnover dataset of human glutamine synthetase","directory":"data/time_resolved_GS_particle_stack/J146","category":"picked particles - multiframe - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3224670,"frames_per_image":75,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/time_resolved_GS_particle_stack/J146.star","picked_particles_directory":"data/time_resolved_GS_particle_stack/J146/extract/*_aligned_DW_particles.mrc","details":"Particle positions are noted in the .star file. The data are picked/extracted particle positions from 5482 micrographs. These particles were template picked and have not be aligned in 2D or 3D.","image_width":"512","image_height":"512"},{"segmentations":[],"name":"Template picked particles from the 239s turnover dataset of human glutamine synthetase","directory":"data/time_resolved_GS_particle_stack/J147","category":"picked particles - multiframe - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3250902,"frames_per_image":75,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/time_resolved_GS_particle_stack/J147.star","picked_particles_directory":"data/time_resolved_GS_particle_stack/J147/extract/*_aligned_DW_particles.mrc","details":"Particle positions are noted in the .star file. The data are picked/extracted particle positions from 5872 micrographs. These particles were template picked and have not be aligned in 2D or 3D.","image_width":"512","image_height":"512"},{"segmentations":[],"name":"Template picked particles from the 99s turnover dataset of human glutamine synthetase","directory":"data/time_resolved_GS_particle_stack/J161","category":"picked particles - multiframe - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1626169,"frames_per_image":75,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/time_resolved_GS_particle_stack/J161.star","picked_particles_directory":"data/time_resolved_GS_particle_stack/J161/extract/*_aligned_DW_particles.mrc","details":"Particle positions are noted in the .star file. The data are picked/extracted particle positions from 2947 micrographs. These particles were template picked and have not be aligned in 2D or 3D.","image_width":"512","image_height":"512"},{"segmentations":[],"name":"Template picked particles from the 122s turnover dataset of human glutamine synthetase","directory":"data/time_resolved_GS_particle_stack/J162","category":"picked particles - multiframe - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":571230,"frames_per_image":75,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/time_resolved_GS_particle_stack/J162.star","picked_particles_directory":"data/time_resolved_GS_particle_stack/J162/extract/*_aligned_DW_particles.mrc","details":"Particle positions are noted in the .star file. The data are picked/extracted particle positions from 1029 micrographs. These particles were template picked and have not be aligned in 2D or 3D.","image_width":"512","image_height":"512"},{"segmentations":[],"name":"Template picked particles from the 517s turnover dataset of human glutamine synthetase","directory":"data/time_resolved_GS_particle_stack/J163","category":"picked particles - multiframe - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":687806,"frames_per_image":75,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/time_resolved_GS_particle_stack/J163.star","picked_particles_directory":"data/time_resolved_GS_particle_stack/J163/extract/*_aligned_DW_particles.mrc","details":"Particle positions are noted in the .star file. The data are picked/extracted particle positions from 1195 micrographs. These particles were template picked and have not be aligned in 2D or 3D.","image_width":"512","image_height":"512"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"F32GM144982","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"R35 GM145238","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"R24GM154186","country":"United States"}],"version_history":[],"title":"Time-resolved cryo-EM particle stacks","principal_investigator":[{"author_orcid":"0000-0003-1717-0914","middle_name":"Raymond","organization":"Department of Chemistry and Biochemistry, San Francisco State University","street":"1600 Holloway Ave","town_or_city":"San Francisco","state_or_province":"CA","post_or_zip":"94312","telephone":null,"fax":null,"first_name":"Eric","last_name":"Greene","email":"egreene [at] sfsu.edu","country":"United States","entry":"EMPIAR-13621"},{"author_orcid":"0000-0002-0350-5827","middle_name":null,"organization":"Department of Biochemistry, University of Washington","street":"1959 NE Pacific Street","town_or_city":"Seattle","state_or_province":"WA","post_or_zip":"98195","telephone":null,"fax":null,"first_name":"Justin","last_name":"Kollman","email":"jkoll [at] uw.edu","country":"United States","entry":"EMPIAR-13621"},{"author_orcid":"0000-0002-5080-2859","middle_name":null,"organization":"Department of Bioengineering and Therapeutic Sciences, University of California San Francisco","street":"600 16th Street","town_or_city":"San Francisco","state_or_province":"CA","post_or_zip":"94143","telephone":null,"fax":null,"first_name":"James","last_name":"Fraser","email":"jfraser [at] fraserlab.com","country":"United States","entry":"EMPIAR-13621"}],"status":"REL","deposition_date":"2026-03-14","release_date":"2026-06-08","obsolete_date":null,"update_date":"2026-06-08","corresponding_author":{"author":{"author_orcid":"0000-0003-1717-0914","middle_name":"Raymond","organization":"Department of Chemistry and Biochemistry, San Francisco State University","street":"1600 Holloway Ave","town_or_city":"San Francisco","state_or_province":"CA","post_or_zip":"94312","first_name":"Eric","last_name":"Greene","country":"United States"}},"authors":[{"author":{"name":"Greene E","author_orcid":"0000-0003-1717-0914"}},{"author":{"name":"Muniz R","author_orcid":null}},{"author":{"name":"Yamamura H","author_orcid":null}},{"author":{"name":"Hoff SE","author_orcid":"0009-0006-8901-221X"}},{"author":{"name":"Bajaj P","author_orcid":"0000-0001-8474-6149"}},{"author":{"name":"Lee DJ","author_orcid":"0000-0002-5132-3243"}},{"author":{"name":"Thompson EM","author_orcid":"0000-0002-6085-3051"}},{"author":{"name":"Arada A","author_orcid":null}},{"author":{"name":"Lee GM","author_orcid":null}},{"author":{"name":"Bonomi M","author_orcid":"0000-0002-7321-0004"}},{"author":{"name":"Kollman JM","author_orcid":"0000-0002-0350-5827"}},{"author":{"name":"Fraser JS","author_orcid":"0000-0002-5080-2859"}}],"cross_references":["EMD-70843"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Greene ER","author_orcid":"0000-0003-1717-0914"},{"name":"Muniz RS","author_orcid":null},{"name":"Kollman JM","author_orcid":"0000-0002-0350-5827"},{"name":"Fraser JS","author_orcid":"0000-0002-5080-2859"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Product-stabilized filamentation by human glutamine synthetase allosterically tunes metabolic activity","volume":null,"country":"United Kingdom","first_page":null,"last_page":null,"year":"2026","language":"English","doi":"10.7554/eLife.108336.1","pubmedid":"40631248","details":"The particle stacks provided were from motion-corrected, dose-weighted, CTF Fit, and extracted with a box size of 512 pixels in cryoSPARC (v3.1; Patch Motion Correction and Patch CTF Fit). Data were collected at a total dosage of 40 e⁻/Ų with a pixel size of 0.86 Å/pixel.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"eLife","issue":null,"preprint":true}],"dataset_size":"8.9 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9oto"],"entry_doi":"10.6019/EMPIAR-13621"}}