{"EMPIAR-13613":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of Leishmania tarentolae step II trans-spliceosome - Dataset 1","directory":"data/Dataset 1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":21415,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 50.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of Leishmania tarentolae step II trans-spliceosome - Dataset 2","directory":"data/Dataset 2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":10577,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 59.0 e-/Å2 (50 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single particle cryo-EM dataset of Leishmania tarentolae step II trans-spliceosome","principal_investigator":[{"author_orcid":"0000-0003-0930-4249","middle_name":null,"organization":"Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Arnaud","last_name":"Vanden Broeck","email":"avanden [at] rockefeller.edu","country":"United States","entry":"EMPIAR-13613"}],"status":"REL","deposition_date":"2026-02-07","release_date":"2026-11-04","obsolete_date":null,"update_date":"2026-11-04","corresponding_author":{"author":{"author_orcid":"0000-0003-0930-4249","middle_name":null,"organization":"Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","first_name":"Arnaud","last_name":"Vanden Broeck","country":"United States"}},"authors":[{"author":{"name":"Vanden Broeck A","author_orcid":"0000-0003-0930-4249"}},{"author":{"name":"Nadenoen T","author_orcid":"0009-0004-3385-5096"}}],"cross_references":["EMD-52844","EMD-52843","EMD-52803","EMD-52796","EMD-52798","EMD-52801","EMD-52808","EMD-52804","EMD-52795","EMD-52805"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Nadenoen T","author_orcid":"0009-0004-3385-5096"},{"name":"Biglione FA","author_orcid":"0000-0001-9536-711X"},{"name":"Vandevenne M","author_orcid":"0009-0007-4780-3459"},{"name":"Vanden Broeck A","author_orcid":"0000-0003-0930-4249"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis of step II spliced leader RNA trans-splicing in trypanosomatid parasites","volume":"17","country":"","first_page":null,"last_page":null,"year":"2026","language":"English","doi":"10.1038/s41467-026-77480-6","pubmedid":"42778629","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"22.5 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9if8","9if7","9ibc"],"entry_doi":"10.6019/EMPIAR-13613"}}